##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BciVI BclI BfrI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaI BseAI BsiWI BsmBI BsmFI
BspDI BspEI BsrDI Bst1107I BstAPI
BstBI BstZ17I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1967 GGTACC G^GTACC
AccI 363 GTCTAC GT^[AC][GT]AC
AcyI 1553 GGCGCC G[GA]^CG[CT]C
AflIII 917 ACATGT A^C[GA][CT]GT
AlwI 298 GGATC GGATC(4/5)
AlwNI 1363 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 732 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1815 AGCGCT AGC^GCT
Asp718I 1967 GGTACC G^GTACC
BalI 1370 TGGCCA TGG^CCA
BbeI 1553 GGCGCC GGCGC^C
BbvCI 75 GCTGAGG CCTCAGC(-5/-2)
BcgI 32 CGACGCGGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 907 CTAG C^TAG
BglI 502 GCCTTACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 871 AGATCT A^GATCT
BsaAI 1512 CACGTA [CT]AC^GT[GA]
BsaHI 1553 GGCGCC G[GA]^CG[CT]C
BsaWI 1580 TCCGGT [AT]^CCGG[AT]
BsgI 1824 GTGCAG GTGCAG(16/14)
Bsp1407I 271 TGTACA T^GTACA
BspLU11I 917 ACATGT A^CATGT
BsrBI 1590 CCGCTC CCGCTC(-3/-3)
BsrGI 271 TGTACA T^GTACA
BssHII 2026 GCGCGC G^CGCGC
BstEII 1492 GGTGACC G^GT[ACGT]ACC
BstPI 1492 GGTGACC G^GT[ACGT]ACC
BstYI 871 AGATCT [GA]^GATC[CT]
Bsu36I 732 CCTGAGG CC^T[ACGT]AGG
DrdI 1530 GACCGCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1815 AGCGCT AGC^GCT
Eco81I 732 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1492 GGTGACC G^GT[ACGT]ACC
EcoRI 281 GAATTC G^AATTC
Hin1I 1553 GGCGCC G[GA]^CG[CT]C
KasI 1553 GGCGCC G^GCGCC
KpnI 1967 GGTACC GGTAC^C
MaeI 907 CTAG C^TAG
MflI 871 AGATCT [GA]^GATC[CT]
MluNI 1370 TGGCCA TGG^CCA
MscI 1370 TGGCCA TGG^CCA
MseI 1249 TTAA T^TAA
NarI 1553 GGCGCC GG^CGCC
PpuMI 587 AGGACCT [GA]G^G[AT]CC[CT]
SacI 955 GAGCTC GAGCT^C
SapI 1575 GCTCTTC GCTCTTC(1/4)
SfoI 1553 GGCGCC GGC^GCC
SspBI 271 TGTACA T^GTACA
StuI 1333 AGGCCT AGG^CCT
Tru9I 1249 TTAA T^TAA
XcmI 923 CCAATGTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 871 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
Alw44I 1404 GTGCAC G^TGCAC
2041 GTGCAC
ApaLI 1404 GTGCAC G^TGCAC
2041 GTGCAC
BsiEI 753 CGACCG CG[GA][CT]^CG
1846 CGGTCG
BsmAI 446 GAGAC GTCTC(1/5)
914 GAGAC
BsmI 723 GCATTC GAATGC(1/-1)
1731 GCATTC
BspHI 636 TCATGA T^CATGA
1313 TCATGA
BstXI 882 CCAGTACACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1956 CCACCGTGCTGG
DsaI 857 CCGTGG C^C[GA][CT]GG
1262 CCGTGG
EcoT14I 1896 CCTTGG C^C[AT][AT]GG
2019 CCAAGG
FauI 1886 CCCGC CCCGC(4/6)
45 GCGGG
MspA1I 1297 CAGCTG C[AC]G^C[GT]G
1622 CAGCTG
NspI 917 ACATGT [GA]CATG^[CT]
1082 GCATGT
PstI 523 CTGCAG CTGCA^G
1619 CTGCAG
PvuII 1297 CAGCTG CAG^CTG
1622 CAGCTG
RcaI 636 TCATGA T^CATGA
1313 TCATGA
StyI 1896 CCTTGG C^C[AT][AT]GG
2019 CCAAGG
TfiI 633 GATTC G^A[AT]TC
1327 GAATC
Tsp45I 428 GTGAC ^GT[GC]AC
1493 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApaI ApoI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII EaeI EarI
EcoNI EcoO109I EcoRII Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MslI MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI Tsp509I TspRI TthHB8I