##################################
# NO CUT ENZYMES ( 50 enzymes) #
##################################
AatII AclI AgeI AhdI Aor51HI
AscI AspEI AviII BcgI BglII
BsiWI BsmBI BspDI BssHII BstBI
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoRV FseI FspI
HindIII KspI MfeI MluI MunI
NheI NotI NruI NspV PacI
PinAI PmeI Psp1406I PvuI RsrII
SacII ScaI SfiI SfuI SgrAI
SnaBI SpeI SphI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 7840 GGTACC G^GTACC
AccIII 3730 TCCGGA T^CCGGA
AflII 3842 CTTAAG C^TTAAG
AseI 7113 ATTAAT AT^TAAT
AsnI 7113 ATTAAT AT^TAAT
Asp700I 6432 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 7840 GGTACC G^GTACC
BbeI 1811 GGCGCC GGCGC^C
BbrPI 4072 CACGTG CAC^GTG
BclI 6988 TGATCA T^GATCA
BfrI 3842 CTTAAG C^TTAAG
BlpI 850 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 850 GCTCAGC GC^T[ACGT]AGC
BsaAI 4072 CACGTG [CT]AC^GT[GA]
BsaBI 117 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3730 TCCGGA T^CCGGA
BsiEI 1482 CGGTCG CG[GA][CT]^CG
BspEI 3730 TCCGGA T^CCGGA
BspLU11I 5988 ACATGT A^CATGT
Bst1107I 1947 GTATAC GTA^TAC
BstZ17I 1947 GTATAC GTA^TAC
CelII 850 GCTCAGC GC^T[ACGT]AGC
FbaI 6988 TGATCA T^GATCA
HpaI 6146 GTTAAC GTT^AAC
KasI 1811 GGCGCC G^GCGCC
KpnI 7840 GGTACC GGTAC^C
MamI 117 GATGTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3730 TCCGGA T^CCGGA
NarI 1811 GGCGCC GG^CGCC
PmaCI 4072 CACGTG CAC^GTG
PmlI 4072 CACGTG CAC^GTG
PshAI 3801 GACCCCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 7113 ATTAAT AT^TAAT
SalI 1484 GTCGAC G^TCGAC
SexAI 1951 ACCAGGT A^CC[AT]GGT
SfoI 1811 GGCGCC GGC^GCC
SspI 755 AATATT AAT^ATT
XmnI 6432 GAAACCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 4073 ACGTGT A^C[GA][CT]GT
5988 ACATGT
BalI 599 TGGCCA TGG^CCA
3865 TGGCCA
BsaI 4568 GAGACC GGTCTC(1/5)
5875 GGTCTC
BsaWI 1480 TCCGGT [AT]^CCGG[AT]
3730 TCCGGA
Bsp1407I 1390 TGTACA T^GTACA
7122 TGTACA
BspHI 2121 TCATGA T^CATGA
3275 TCATGA
BsrGI 1390 TGTACA T^GTACA
7122 TGTACA
BstXI 1300 CCAGGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4394 CCAGGATGCTGG
EcoRI 4596 GAATTC G^AATTC
4952 GAATTC
HincII 1484 GTCGAC GT[CT]^[GA]AC
6146 GTTAAC
HindII 1484 GTCGAC GT[CT]^[GA]AC
6146 GTTAAC
MluNI 599 TGGCCA TGG^CCA
3865 TGGCCA
MscI 599 TGGCCA TGG^CCA
3865 TGGCCA
NaeI 1675 GCCGGC GCC^GGC
4135 GCCGGC
NgoMIV 1675 GCCGGC G^CCGGC
4135 GCCGGC
RcaI 2121 TCATGA T^CATGA
3275 TCATGA
SapI 5853 GAAGAGC GCTCTTC(1/4)
7092 GCTCTTC
SbfI 2534 CCTGCAGG CCTGCA^GG
3002 CCTGCAGG
Sse8387I 2534 CCTGCAGG CCTGCA^GG
3002 CCTGCAGG
SspBI 1390 TGTACA T^GTACA
7122 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 150 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI Alw44I AlwI AlwNI
AocI ApaI ApaLI ApoI AspHI
AspI AvaI AvaII AvrII BamHI
BanI BanII BbsI BbvCI BbvI
BciVI BcnI BfaI BglI BlnI
BmyI BpmI BpuAI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrI
BssKI BssSI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstYI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DrdI
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoO65I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeI NdeII NlaIII NlaIV
NsiI NspI PaeR7I PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI