##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AclI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp718I
BamHI BbrPI BbvCI BsaBI BsiWI
BsrBI BssHII Bst1107I BstAPI BstZ17I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoNI EcoRV FseI HgaI
HpaI KpnI KspI MamI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI PflMI PmaCI PmeI
PmlI PshBI Psp1406I RsrII SacII
SalI SexAI SfiI SgrAI SmaI
SnaBI SwaI Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 2120 TCCGGA T^CCGGA
AflII 4482 CTTAAG C^TTAAG
AgeI 3559 ACCGGT A^CCGGT
AhdI 1305 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1242 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 1610 GGGCCC GGGCC^C
AspEI 1305 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1598 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1347 TGCGCA TGC^GCA
BalI 2502 TGGCCA TGG^CCA
BcgI 1883 CGAAGTAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4482 CTTAAG C^TTAAG
BglII 625 AGATCT A^GATCT
BlpI 1200 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1200 GCTGAGC GC^T[ACGT]AGC
BsaAI 4458 CACGTA [CT]AC^GT[GA]
BseAI 2120 TCCGGA T^CCGGA
BsmBI 2560 CGTCTC CGTCTC(1/5)
Bsp1407I 3638 TGTACA T^GTACA
BspDI 357 ATCGAT AT^CGAT
BspEI 2120 TCCGGA T^CCGGA
BspHI 2267 TCATGA T^CATGA
BsrFI 3559 ACCGGT [GA]^CCGG[CT]
BsrGI 3638 TGTACA T^GTACA
BssSI 1086 CACGAG CACGAG(-5/-1)
CelII 1200 GCTGAGC GC^T[ACGT]AGC
Cfr10I 3559 ACCGGT [GA]^CCGG[CT]
ClaI 357 ATCGAT AT^CGAT
DrdI 3750 GACATTTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1305 GACCCAGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1347 TGCGCA TGC^GCA
HindIII 4606 AAGCTT A^AGCTT
MluNI 2502 TGGCCA TGG^CCA
MroI 2120 TCCGGA T^CCGGA
MscI 2502 TGGCCA TGG^CCA
PacI 4592 TTAATTAA TTAAT^TAA
PaeR7I 3992 CTCGAG C^TCGAG
PflFI 1598 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 3559 ACCGGT A^CCGGT
PshAI 3827 GACCGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 286 CGATCG CGAT^CG
RcaI 2267 TCATGA T^CATGA
SacI 1059 GAGCTC GAGCT^C
SapI 387 GAAGAGC GCTCTTC(1/4)
SbfI 380 CCTGCAGG CCTGCA^GG
ScaI 4341 AGTACT AGT^ACT
Sse8387I 380 CCTGCAGG CCTGCA^GG
SspBI 3638 TGTACA T^GTACA
Tth111I 1598 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1497 CCAACAGTCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 3992 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccI 1331 GTAGAC GT^[AC][GT]AC
4262 GTAGAC
AccII 751 CGCG CG^CG
1418 CGCG
AcyI 1830 GGCGCC G[GA]^CG[CT]C
3618 GGCGCC
AlwI 398 GATCC GGATC(4/5)
845 GGATC
AocI 1672 CCTGAGG CC^T[ACGT]AGG
1781 CCTCAGG
AvaI 2106 CTCGGG C^[CT]CG[GA]G
3992 CTCGAG
AvrII 986 CCTAGG C^CTAGG
4141 CCTAGG
BbeI 1830 GGCGCC GGCGC^C
3618 GGCGCC
BclI 2841 TGATCA T^GATCA
4215 TGATCA
BglI 1394 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2782 GCCCCACTGGC
BlnI 986 CCTAGG C^CTAGG
4141 CCTAGG
BsaHI 1830 GGCGCC G[GA]^CG[CT]C
3618 GGCGCC
BsiEI 1 CGACCG CG[GA][CT]^CG
286 CGATCG
BsmI 1172 GAATGC GAATGC(1/-1)
4558 GCATTC
BsoBI 2106 CTCGGG C^[CT]CG[GA]G
3992 CTCGAG
BspLU11I 56 ACATGT A^CATGT
4564 ACATGT
BstBI 192 TTCGAA TT^CGAA
560 TTCGAA
BstUI 751 CGCG CG^CG
1418 CGCG
Bsu36I 1672 CCTGAGG CC^T[ACGT]AGG
1781 CCTCAGG
EaeI 1479 TGGCCG [CT]^GGCC[GA]
2502 TGGCCA
Eco81I 1672 CCTGAGG CC^T[ACGT]AGG
1781 CCTCAGG
EcoRI 351 GAATTC G^AATTC
2078 GAATTC
FbaI 2841 TGATCA T^GATCA
4215 TGATCA
HaeII 1830 GGCGCC [GA]GCGC^[CT]
3618 GGCGCC
Hin1I 1830 GGCGCC G[GA]^CG[CT]C
3618 GGCGCC
KasI 1830 GGCGCC G^GCGCC
3618 GGCGCC
MvnI 751 CGCG CG^CG
1418 CGCG
NarI 1830 GGCGCC GG^CGCC
3618 GGCGCC
NcoI 1691 CCATGG C^CATGG
2588 CCATGG
NspV 192 TTCGAA TT^CGAA
560 TTCGAA
PstI 381 CTGCAG CTGCA^G
4510 CTGCAG
SfoI 1830 GGCGCC GGC^GCC
3618 GGCGCC
SfuI 192 TTCGAA TT^CGAA
560 TTCGAA
SspI 2037 AATATT AAT^ATT
2386 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI Asp700I AspHI AvaII BanI
BanII BbsI BbvI BciVI BcnI
BfaI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoO65I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeI NdeII NlaIII
NlaIV NsiI NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SpeI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI