##################################
# NO CUT ENZYMES ( 58 enzymes) #
##################################
AatII AccIII AgeI AscI AspI
AviII BcgI BciVI BsaBI BsaWI
BseAI BseRI BsiWI BsmBI BspDI
BspEI BspLU11I BsrDI Bst1107I BstBI
BstZ17I ClaI CpoI EcoRI EcoRV
EcoT22I FseI FspI HindIII HpaI
MamI MluI MroI NcoI NdeI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI RsrII
SalI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 752 GGTACC G^GTACC
AclI 1725 AACGTT AA^CGTT
AflII 701 CTTAAG C^TTAAG
AflIII 2505 ACGTGT A^C[GA][CT]GT
AhdI 3442 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 206 GTGCAC G^TGCAC
AocI 2562 CCTTAGG CC^T[ACGT]AGG
ApaLI 206 GTGCAC G^TGCAC
Asp700I 3407 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 752 GGTACC G^GTACC
AspEI 3442 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 823 GGATCC G^GATCC
BbrPI 209 CACGTG CAC^GTG
BclI 4317 TGATCA T^GATCA
BfrI 701 CTTAAG C^TTAAG
BglII 2425 AGATCT A^GATCT
BsaAI 209 CACGTG [CT]AC^GT[GA]
Bsp1407I 438 TGTACA T^GTACA
BspHI 480 TCATGA T^CATGA
BsrBI 1280 CCGCTC CCGCTC(-3/-3)
BsrGI 438 TGTACA T^GTACA
BstEII 2413 GGTCACC G^GT[ACGT]ACC
BstPI 2413 GGTCACC G^GT[ACGT]ACC
Bsu36I 2562 CCTTAGG CC^T[ACGT]AGG
DraIII 176 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 3442 GACCCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2562 CCTTAGG CC^T[ACGT]AGG
EcoO65I 2413 GGTCACC G^GT[ACGT]ACC
FbaI 4317 TGATCA T^GATCA
HincII 986 GTTGAC GT[CT]^[GA]AC
HindII 986 GTTGAC GT[CT]^[GA]AC
KpnI 752 GGTACC GGTAC^C
KspI 1048 CCGCGG CCGC^GG
MfeI 3829 CAATTG C^AATTG
MunI 3829 CAATTG C^AATTG
NheI 2795 GCTAGC G^CTAGC
NspI 3991 GCATGC [GA]CATG^[CT]
PmaCI 209 CACGTG CAC^GTG
PmlI 209 CACGTG CAC^GTG
Psp1406I 1725 AACGTT AA^CGTT
RcaI 480 TCATGA T^CATGA
SacI 107 GAGCTC GAGCT^C
SacII 1048 CCGCGG CCGC^GG
ScaI 1119 AGTACT AGT^ACT
SmaI 2481 CCCGGG CCC^GGG
SphI 3991 GCATGC GCATG^C
SspBI 438 TGTACA T^GTACA
StuI 3693 AGGCCT AGG^CCT
XmaCI 2481 CCCGGG C^CCGGG
XmaI 2481 CCCGGG C^CCGGG
XmnI 3407 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 3655 GTCTAC GT^[AC][GT]AC
4353 GTAGAC
Aor51HI 388 AGCGCT AGC^GCT
1104 AGCGCT
AseI 2351 ATTAAT AT^TAAT
3172 ATTAAT
AsnI 2351 ATTAAT AT^TAAT
3172 ATTAAT
AvaI 590 CTCGGG C^[CT]CG[GA]G
2481 CCCGGG
AvrII 1335 CCTAGG C^CTAGG
1507 CCTAGG
BbeI 1004 GGCGCC GGCGC^C
1044 GGCGCC
BbsI 1187 GTCTTC GAAGAC(2/6)
2171 GTCTTC
BglI 885 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1333 GCCCTAGGGGC
BlnI 1335 CCTAGG C^CTAGG
1507 CCTAGG
BlpI 104 GCTGAGC GC^T[ACGT]AGC
2634 GCTAAGC
Bpu1102I 104 GCTGAGC GC^T[ACGT]AGC
2634 GCTAAGC
BpuAI 1187 GTCTTC GAAGAC(2/6)
2171 GTCTTC
BsaI 3440 GAGACC GGTCTC(1/5)
3564 GGTCTC
BsoBI 590 CTCGGG C^[CT]CG[GA]G
2481 CCCGGG
BstAPI 565 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
711 GCATAAACTGC
BstXI 2234 CCACAAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2732 CCAGCTGTCTGG
CelII 104 GCTGAGC GC^T[ACGT]AGC
2634 GCTAAGC
DrdI 1759 GACTCTTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2079 GACTCTCCAGTC
EagI 967 CGGCCG C^GGCCG
1059 CGGCCG
EclXI 967 CGGCCG C^GGCCG
1059 CGGCCG
Eco47III 388 AGCGCT AGC^GCT
1104 AGCGCT
Eco52I 967 CGGCCG C^GGCCG
1059 CGGCCG
KasI 1004 GGCGCC G^GCGCC
1044 GGCGCC
MslI 2136 CACATTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4273 CACAGGAATG
NaeI 1007 GCCGGC GCC^GGC
1205 GCCGGC
NarI 1004 GGCGCC GG^CGCC
1044 GGCGCC
NgoMIV 1007 GCCGGC G^CCGGC
1205 GCCGGC
PflMI 175 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3573 CCAGTGCCTGG
PshAI 3648 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4110 GACTCTTGTC
PshBI 2351 ATTAAT AT^TAAT
3172 ATTAAT
PstI 3145 CTGCAG CTGCA^G
3306 CTGCAG
PvuI 785 CGATCG CGAT^CG
862 CGATCG
SapI 2386 GAAGAGC GCTCTTC(1/4)
109 GCTCTTC
SfoI 1004 GGCGCC GGC^GCC
1044 GGCGCC
SspI 4262 AATATT AAT^ATT
4417 AATATT
Van91I 175 CCACCCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3573 CCAGTGCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsgI BsiEI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspMI BsrFI BsrI
BssHII BssKI BssSI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XcmI XhoII