##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I ApaLI AscI AseI
AsnI Asp718I AspEI AviII AvrII
BamHI BbrPI BciVI BfrI BlnI
BsaAI BsaBI BsiEI BsiWI BspDI
BssHII Bst1107I BstZ17I ClaI DraI
EagI Eam1105I EclXI Eco52I EcoRV
FseI FspI HpaI KpnI KspI
MamI MfeI MluI MunI NcoI
NdeI NheI NotI NruI PacI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI SacII SalI SapI
SbfI SexAI SfiI SgrAI SnaBI
SpeI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccI 2294 GTCTAC GT^[AC][GT]AC
AflIII 378 ACATGT A^C[GA][CT]GT
AspI 850 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 3091 GGCGCC GGCGC^C
BbsI 2585 GAAGAC GAAGAC(2/6)
BcgI 2637 CGAAGTTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2064 TGATCA T^GATCA
BglI 2501 GCCAAGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 924 AGATCT A^GATCT
BpuAI 2585 GAAGAC GAAGAC(2/6)
Bsp1407I 1148 TGTACA T^GTACA
BspHI 2067 TCATGA T^CATGA
BspLU11I 378 ACATGT A^CATGT
BsrGI 1148 TGTACA T^GTACA
BssSI 3455 CTCGTG CACGAG(-5/-1)
BstBI 2635 TTCGAA TT^CGAA
BstEII 3292 GGTCACC G^GT[ACGT]ACC
BstPI 3292 GGTCACC G^GT[ACGT]ACC
CpoI 1233 CGGACCG CG^G[AT]CCG
DrdI 344 GACCTGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 2890 CCGTGG C^C[GA][CT]GG
EcoO65I 3292 GGTCACC G^GT[ACGT]ACC
EcoRI 1490 GAATTC G^AATTC
EcoT22I 421 ATGCAT ATGCA^T
FbaI 2064 TGATCA T^GATCA
HindIII 933 AAGCTT A^AGCTT
KasI 3091 GGCGCC G^GCGCC
MseI 2319 TTAA T^TAA
NaeI 515 GCCGGC GCC^GGC
NarI 3091 GGCGCC GG^CGCC
NgoMIV 515 GCCGGC G^CCGGC
NsiI 421 ATGCAT ATGCA^T
NspV 2635 TTCGAA TT^CGAA
PaeR7I 821 CTCGAG C^TCGAG
PflFI 850 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 2067 TCATGA T^CATGA
RsrII 1233 CGGACCG CG^G[AT]CCG
ScaI 1038 AGTACT AGT^ACT
SfoI 3091 GGCGCC GGC^GCC
SfuI 2635 TTCGAA TT^CGAA
SmaI 2485 CCCGGG CCC^GGG
SspBI 1148 TGTACA T^GTACA
Tru9I 2319 TTAA T^TAA
Tth111I 850 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3349 TCTAGA T^CTAGA
XhoI 821 CTCGAG C^TCGAG
XmaCI 2485 CCCGGG C^CCGGG
XmaI 2485 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccIII 705 TCCGGA T^CCGGA
1956 TCCGGA
AocI 1673 CCTGAGG CC^T[ACGT]AGG
3255 CCTGAGG
Aor51HI 120 AGCGCT AGC^GCT
1131 AGCGCT
ApaI 597 GGGCCC GGGCC^C
1539 GGGCCC
BalI 2775 TGGCCA TGG^CCA
2976 TGGCCA
BlpI 1220 GCTAAGC GC^T[ACGT]AGC
2653 GCTGAGC
Bpu1102I 1220 GCTAAGC GC^T[ACGT]AGC
2653 GCTGAGC
BsaI 1649 GAGACC GGTCTC(1/5)
2811 GGTCTC
BseAI 705 TCCGGA T^CCGGA
1956 TCCGGA
BsmBI 848 GAGACG CGTCTC(1/5)
2336 GAGACG
BspEI 705 TCCGGA T^CCGGA
1956 TCCGGA
BsrDI 360 CATTGC GCAATG(2/0)
3028 CATTGC
Bsu36I 1673 CCTGAGG CC^T[ACGT]AGG
3255 CCTGAGG
CelII 1220 GCTAAGC GC^T[ACGT]AGC
2653 GCTGAGC
DraIII 806 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
3147 CACATGGTG
Eco47III 120 AGCGCT AGC^GCT
1131 AGCGCT
Eco81I 1673 CCTGAGG CC^T[ACGT]AGG
3255 CCTGAGG
MaeII 1953 ACGT A^CGT
2100 ACGT
MluNI 2775 TGGCCA TGG^CCA
2976 TGGCCA
MroI 705 TCCGGA T^CCGGA
1956 TCCGGA
MscI 2775 TGGCCA TGG^CCA
2976 TGGCCA
PflMI 524 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3242 CCATCTGCTGG
PshAI 2410 GACCCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2550 GACAGGCGTC
SacI 977 GAGCTC GAGCT^C
2656 GAGCTC
StuI 627 AGGCCT AGG^CCT
1800 AGGCCT
TfiI 1009 GATTC G^A[AT]TC
1606 GATTC
Van91I 524 CCAGCCGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3242 CCATCTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BmyI
BpmI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BstAPI
BstF5I BstNI BstUI BstXI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmnI