##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII AccI AflII AgeI AhdI
AscI AseI AsnI AspEI AviII
BcgI BfrI BglII BsiWI BsmI
BspDI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI Eam1105I EcoO65I EcoRV FseI
FspI HindIII HpaI MfeI MunI
NaeI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmeI PshBI PvuI RsrII SacI
SalI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
Acc65I 75 GGTACC G^GTACC
AclI 3833 AACGTT AA^CGTT
Aor51HI 559 AGCGCT AGC^GCT
Asp700I 2775 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 75 GGTACC G^GTACC
AspI 4328 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4023 CCTAGG C^CTAGG
BamHI 1383 GGATCC G^GATCC
BbeI 4209 GGCGCC GGCGC^C
BclI 251 TGATCA T^GATCA
BlnI 4023 CCTAGG C^CTAGG
BlpI 2925 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 2925 GCTTAGC GC^T[ACGT]AGC
BsaBI 1804 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 3443 CGGCCG CG[GA][CT]^CG
BspHI 69 TCATGA T^CATGA
BspLU11I 2426 ACATGT A^CATGT
CelII 2925 GCTTAGC GC^T[ACGT]AGC
DraIII 818 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1196 GACCTCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 663 CCGCGG C^C[GA][CT]GG
EagI 3443 CGGCCG C^GGCCG
EclXI 3443 CGGCCG C^GGCCG
Eco47III 559 AGCGCT AGC^GCT
Eco52I 3443 CGGCCG C^GGCCG
EcoRI 3577 GAATTC G^AATTC
EcoT22I 1336 ATGCAT ATGCA^T
FbaI 251 TGATCA T^GATCA
HincII 4315 GTTGAC GT[CT]^[GA]AC
HindII 4315 GTTGAC GT[CT]^[GA]AC
KasI 4209 GGCGCC G^GCGCC
KpnI 75 GGTACC GGTAC^C
KspI 663 CCGCGG CCGC^GG
MamI 1804 GATGCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluI 389 ACGCGT A^CGCGT
NarI 4209 GGCGCC GG^CGCC
NsiI 1336 ATGCAT ATGCA^T
PflFI 4328 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 3833 AACGTT AA^CGTT
RcaI 69 TCATGA T^CATGA
SacII 663 CCGCGG CCGC^GG
SfoI 4209 GGCGCC GGC^GCC
SmaI 4261 CCCGGG CCC^GGG
SphI 2622 GCATGC GCATG^C
SspI 236 AATATT AAT^ATT
Tth111I 4328 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 4261 CCCGGG C^CCGGG
XmaI 4261 CCCGGG C^CCGGG
XmnI 2775 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 1329 TCCGGA T^CCGGA
1403 TCCGGA
Alw44I 910 GTGCAC G^TGCAC
3805 GTGCAC
AocI 3097 CCTGAGG CC^T[ACGT]AGG
4040 CCTGAGG
ApaLI 910 GTGCAC G^TGCAC
3805 GTGCAC
BbrPI 2824 CACGTG CAC^GTG
3308 CACGTG
BbsI 2730 GAAGAC GAAGAC(2/6)
1930 GTCTTC
BciVI 2313 GGATAC GTATCC(6/5)
3897 GTATCC
BpuAI 2730 GAAGAC GAAGAC(2/6)
1930 GTCTTC
BsaAI 2824 CACGTG [CT]AC^GT[GA]
3308 CACGTG
BseAI 1329 TCCGGA T^CCGGA
1403 TCCGGA
Bsp1407I 2573 TGTACA T^GTACA
3877 TGTACA
BspEI 1329 TCCGGA T^CCGGA
1403 TCCGGA
BsrDI 1354 CATTGC GCAATG(2/0)
1257 GCAATG
BsrGI 2573 TGTACA T^GTACA
3877 TGTACA
BstYI 1002 AGATCC [GA]^GATC[CT]
1383 GGATCC
Bsu36I 3097 CCTGAGG CC^T[ACGT]AGG
4040 CCTGAGG
Eco81I 3097 CCTGAGG CC^T[ACGT]AGG
4040 CCTGAGG
EcoNI 2346 CCTGAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3080 CCTGCTGCAGG
MflI 1002 AGATCC [GA]^GATC[CT]
1383 GGATCC
MroI 1329 TCCGGA T^CCGGA
1403 TCCGGA
NheI 1768 GCTAGC G^CTAGC
2187 GCTAGC
PflMI 820 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2680 CCACAGGATGG
PmaCI 2824 CACGTG CAC^GTG
3308 CACGTG
PmlI 2824 CACGTG CAC^GTG
3308 CACGTG
PshAI 1363 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2733 GACATCTGTC
SapI 1627 GAAGAGC GCTCTTC(1/4)
1237 GCTCTTC
SexAI 819 ACCAGGT A^CC[AT]GGT
2072 ACCTGGT
SfiI 1499 GGCCGGGCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1508 GGCCGCACGGGCC
SspBI 2573 TGTACA T^GTACA
3877 TGTACA
Van91I 820 CCAGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2680 CCACAGGATGG
XcmI 1821 CCATGTTCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2207 CCATCCGACTCCTGG
XhoII 1002 AGATCC [GA]^GATC[CT]
1383 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI ApaI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaI BsaJI BsaWI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I