##################################
# NO CUT ENZYMES ( 111 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI Asp718I AspEI
AspHI AviII AvrII BamHI BanI
BanII BbeI BbrPI BcnI BglI
BglII BlnI BmyI BpmI BsaHI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1286I BspDI BspEI BsrBI
BsrFI BssSI Bst1107I BstEII BstPI
BstXI BstYI BstZ17I Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoO109I EcoO65I
EcoT14I FseI FspI HaeII Hin1I
KasI KpnI MflI MluI MroI
NaeI NarI NciI NcoI NgoMIV
NheI NotI NruI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PvuI RsrII
SacI SalI SapI SbfI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SphI Sse8387I StuI StyI SwaI
Van91I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AflII 1089 CTTAAG C^TTAAG
AlwNI 130 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 699 CCCGAG C^[CT]CG[GA]G
BbvCI 65 CCTCAGC CCTCAGC(-5/-2)
BfrI 1089 CTTAAG C^TTAAG
BlpI 275 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 275 GCTAAGC GC^T[ACGT]AGC
BsaWI 1469 ACCGGA [AT]^CCGG[AT]
BseRI 2670 GAGGAG GAGGAG(10/8)
BsoBI 699 CCCGAG C^[CT]CG[GA]G
BspLU11I 2408 ACATGT A^CATGT
BspMI 1796 ACCTGC ACCTGC(4/8)
BsrDI 3931 CATTGC GCAATG(2/0)
BssHII 70 GCGCGC G^CGCGC
BstAPI 2100 GCAGATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 869 TTCGAA TT^CGAA
CelII 275 GCTAAGC GC^T[ACGT]AGC
EcoNI 3645 CCTTTTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 694 GAATTC G^AATTC
EcoRV 687 GATATC GAT^ATC
EcoT22I 2983 ATGCAT ATGCA^T
HapII 1470 CCGG C^CGG
HincII 2724 GTTAAC GT[CT]^[GA]AC
HindII 2724 GTTAAC GT[CT]^[GA]AC
HpaI 2724 GTTAAC GTT^AAC
HpaII 1470 CCGG C^CGG
KspI 53 CCGCGG CCGC^GG
MfeI 4131 CAATTG C^AATTG
MspI 1470 CCGG C^CGG
MunI 4131 CAATTG C^AATTG
NsiI 2983 ATGCAT ATGCA^T
NspV 869 TTCGAA TT^CGAA
SacII 53 CCGCGG CCGC^GG
SfuI 869 TTCGAA TT^CGAA
SpeI 105 ACTAGT A^CTAGT
XcmI 3244 CCATAATACAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 54 CGCG CG^CG
71 CGCG
AflIII 2408 ACATGT A^C[GA][CT]GT
3997 ACGTGT
AspI 294 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2179 GACAGTGTC
BcgI 2090 CGATAAATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3233 CGAGGGCTATGC
BciVI 58 GGATAC GTATCC(6/5)
2224 GGATAC
BclI 1253 TGATCA T^GATCA
2198 TGATCA
BsaAI 838 TACGTG [CT]AC^GT[GA]
3996 TACGTG
BsaBI 897 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2760 GATCTTGATC
BsmAI 1664 GAGAC GTCTC(1/5)
425 GTCTC
Bsp1407I 2900 TGTACA T^GTACA
3638 TGTACA
BsrGI 2900 TGTACA T^GTACA
3638 TGTACA
BstUI 54 CGCG CG^CG
71 CGCG
DraIII 2581 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3479 CACAAAGTG
DsaI 53 CCGCGG C^C[GA][CT]GG
562 CCACGG
EarI 2716 CTCTTC CTCTTC(1/4)
872 GAAGAG
FauI 52 CCCGC CCCGC(4/6)
55 GCGGG
FbaI 1253 TGATCA T^GATCA
2198 TGATCA
Ksp632I 2716 CTCTTC CTCTTC(1/4)
872 GAAGAG
MamI 897 GATGTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2760 GATCTTGATC
MvnI 54 CGCG CG^CG
71 CGCG
PflFI 294 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2179 GACAGTGTC
PvuII 861 CAGCTG CAG^CTG
3061 CAGCTG
ScaI 2847 AGTACT AGT^ACT
3140 AGTACT
SspBI 2900 TGTACA T^GTACA
3638 TGTACA
Tth111I 294 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2179 GACAGTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AocI ApoI AseI AsnI Asp700I
AvaII BalI BbsI BbvI BfaI
BpuAI BsaJI BsgI BsiYI BslI
BsmFI BsmI BspHI BsrI BssKI
BstF5I BstNI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI EaeI
Eco81I EcoRII Fnu4HI FokI HaeIII
HgaI HhaI HinP1I HindIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NdeI NdeII NlaIII NlaIV NspI
PleI PshBI PstI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI