##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AcyI AocI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BbrPI BcgI BclI BglI BglII
BlnI BlpI Bpu1102I BsaHI BsaI
BseAI BsgI BsiWI BsmI BspEI
BspMI BsrBI BssHII BssSI BstAPI
BstBI BstEII BstPI Bsu36I CelII
CpoI DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoT22I FbaI FseI FspI Hin1I
HpaI KasI KpnI KspI MfeI
MluI MroI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PflFI
PmaCI PmeI PmlI PshAI PshBI
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SpeI SphI Sse8387I
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 4114 GTATAC GT^[AC][GT]AC
AccII 16 CGCG CG^CG
AgeI 2205 ACCGGT A^CCGGT
AhdI 3935 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2278 GTGCAC G^TGCAC
AlwNI 793 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2278 GTGCAC G^TGCAC
AspEI 3935 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 531 TGGCCA TGG^CCA
BamHI 270 GGATCC G^GATCC
BbsI 3391 GTCTTC GAAGAC(2/6)
BbvCI 10 CCTCAGC CCTCAGC(-5/-2)
BpuAI 3391 GTCTTC GAAGAC(2/6)
BsaBI 1744 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 614 CTCCTC GAGGAG(10/8)
BsiEI 658 CGACCG CG[GA][CT]^CG
BsmBI 167 CGTCTC CGTCTC(1/5)
BspHI 1293 TCATGA T^CATGA
Bst1107I 4114 GTATAC GTA^TAC
BstUI 16 CGCG CG^CG
BstXI 1937 CCAAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4114 GTATAC GTA^TAC
DsaI 3857 CCACGG C^C[GA][CT]GG
Eam1105I 3935 GACACATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 3118 GAATTC G^AATTC
HaeII 1030 GGCGCT [GA]GCGC^[CT]
HgaI 2240 GACGC GACGC(5/10)
HindIII 402 AAGCTT A^AGCTT
MamI 1744 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 531 TGGCCA TGG^CCA
MscI 531 TGGCCA TGG^CCA
MvnI 16 CGCG CG^CG
PaeR7I 3009 CTCGAG C^TCGAG
PflMI 792 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2205 ACCGGT A^CCGGT
PstI 1620 CTGCAG CTGCA^G
RcaI 1293 TCATGA T^CATGA
SacI 4074 GAGCTC GAGCT^C
SmaI 177 CCCGGG CCC^GGG
SnaBI 3851 TACGTA TAC^GTA
StuI 3193 AGGCCT AGG^CCT
Van91I 792 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 3635 TCTAGA T^CTAGA
XhoI 3009 CTCGAG C^TCGAG
XmaCI 177 CCCGGG C^CCGGG
XmaI 177 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
Asp700I 3708 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4099 GAATAAATTC
BanII 180 GGGCTC G[GA]GC[CT]^C
4074 GAGCTC
BciVI 1692 GGATAC GTATCC(6/5)
2849 GGATAC
BpmI 308 CTGGAG CTGGAG(16/14)
1581 CTGGAG
BsaAI 1400 TACGTG [CT]AC^GT[GA]
3851 TACGTA
BsaWI 438 ACCGGA [AT]^CCGG[AT]
2205 ACCGGT
BspDI 992 ATCGAT AT^CGAT
2267 ATCGAT
BspLU11I 3018 ACATGT A^CATGT
3817 ACATGT
BsrDI 247 GCAATG GCAATG(2/0)
1733 GCAATG
BsrFI 44 GCCGGT [GA]^CCGG[CT]
2205 ACCGGT
CfoI 15 GCGC GCG^C
1031 GCGC
Cfr10I 44 GCCGGT [GA]^CCGG[CT]
2205 ACCGGT
ClaI 992 ATCGAT AT^CGAT
2267 ATCGAT
DraII 199 AGGTCCC [GA]G^G[ACGT]CC[CT]
794 AGGACCT
EaeI 194 CGGCCA [CT]^GGCC[GA]
531 TGGCCA
EarI 978 GAAGAG CTCTTC(1/4)
1342 GAAGAG
EcoO109I 199 AGGTCCC [GA]G^G[ACGT]CC[CT]
794 AGGACCT
EcoRV 1823 GATATC GAT^ATC
2427 GATATC
EcoT14I 1688 CCTTGG C^C[AT][AT]GG
2225 CCAAGG
FauI 23 CCCGC CCCGC(4/6)
38 GCGGG
HhaI 15 GCGC GCG^C
1031 GCGC
HinP1I 15 GCGC G^CGC
1031 GCGC
HincII 1275 GTTGAC GT[CT]^[GA]AC
3469 GTTGAC
HindII 1275 GTTGAC GT[CT]^[GA]AC
3469 GTTGAC
Ksp632I 978 GAAGAG CTCTTC(1/4)
1342 GAAGAG
PpuMI 199 AGGTCCC [GA]G^G[AT]CC[CT]
794 AGGACCT
PvuII 2284 CAGCTG CAG^CTG
4094 CAGCTG
StyI 1688 CCTTGG C^C[AT][AT]GG
2225 CCAAGG
XcmI 114 CCAAAGTAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
561 CCAGGGTGGAAATGG
XmnI 3708 GAACGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4099 GAATAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AclI AcsI AfaI AflII
AflIII AluI AlwI ApoI AspHI
AvaI AvaII BanI BbvI BcnI
BfaI BfrI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EcoRII Fnu4HI
FokI HaeIII HapII HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI Psp1406I RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII