##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII Acc65I AccIII AgeI Alw44I
ApaLI AscI Asp718I BalI BbeI
BbrPI BcgI BseAI BsiEI Bsp1407I
BspEI BspHI BspLU11I BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraIII EaeI EagI EclXI
Eco52I EcoO65I EcoRV FseI HpaI
KasI KpnI KspI MluNI MroI
MscI NarI NdeI NheI NotI
NruI NspV PacI PinAI PmaCI
PmeI PmlI PvuI RcaI RsrII
SacI SacII SalI SbfI SfiI
SfoI SfuI SgrAI SpeI Sse8387I
SspBI SwaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 1082 GTCTAC GT^[AC][GT]AC
AclI 2233 AACGTT AA^CGTT
AflII 4719 CTTAAG C^TTAAG
AflIII 693 ACGCGT A^C[GA][CT]GT
AlwNI 2842 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3042 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2262 AGCGCT AGC^GCT
ApaI 3539 GGGCCC GGGCC^C
AspHI 1818 GAGCAC G[AT]GC[AT]^C
AviII 4905 TGCGCA TGC^GCA
AvrII 3852 CCTAGG C^CTAGG
BciVI 4822 GGATAC GTATCC(6/5)
BfrI 4719 CTTAAG C^TTAAG
BglII 3989 AGATCT A^GATCT
BlnI 3852 CCTAGG C^CTAGG
BlpI 111 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC
BsaAI 4732 TACGTA [CT]AC^GT[GA]
BsaWI 1391 ACCGGA [AT]^CCGG[AT]
BsiHKAI 1818 GAGCAC G[AT]GC[AT]^C
BsiWI 1131 CGTACG C^GTACG
BspDI 2494 ATCGAT AT^CGAT
BsrDI 1557 CATTGC GCAATG(2/0)
BssHII 2618 GCGCGC G^CGCGC
Bsu36I 3042 CCTGAGG CC^T[ACGT]AGG
CelII 111 GCTGAGC GC^T[ACGT]AGC
ClaI 2494 ATCGAT AT^CGAT
DrdI 1697 GACAGCCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 570 CCATGG C^C[GA][CT]GG
Eco47III 2262 AGCGCT AGC^GCT
Eco81I 3042 CCTGAGG CC^T[ACGT]AGG
FspI 4905 TGCGCA TGC^GCA
HindIII 667 AAGCTT A^AGCTT
MluI 693 ACGCGT A^CGCGT
MslI 3159 CATGCGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 570 CCATGG C^CATGG
NspI 1666 GCATGC [GA]CATG^[CT]
Psp1406I 2233 AACGTT AA^CGTT
PstI 1446 CTGCAG CTGCA^G
ScaI 1712 AGTACT AGT^ACT
SexAI 3623 ACCTGGT A^CC[AT]GGT
SmaI 76 CCCGGG CCC^GGG
SnaBI 4732 TACGTA TAC^GTA
SphI 1666 GCATGC GCATG^C
XmaCI 76 CCCGGG C^CCGGG
XmaI 76 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AhdI 3637 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4270 GACGTTAAGTC
AspEI 3637 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4270 GACGTTAAGTC
AspI 3034 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3622 GACCTGGTC
BamHI 1828 GGATCC G^GATCC
2120 GGATCC
BanI 2073 GGTGCC G^G[CT][GA]CC
2817 GGCACC
BanII 2967 GAGCCC G[GA]GC[CT]^C
3539 GGGCCC
BbvCI 1947 GCTGAGG CCTCAGC(-5/-2)
3885 GCTGAGG
BglI 2 GCCGGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3086 GCCTTCCTGGC
BsgI 2425 CTGCAC GTGCAG(16/14)
3523 GTGCAG
BspMI 1451 GCAGGT ACCTGC(4/8)
1568 GCAGGT
BstAPI 1403 GCAGTATGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1661 GCAAAGCATGC
Eam1105I 3637 GACCATTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4270 GACGTTAAGTC
EcoNI 3180 CCTAGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4738 CCTATTTTAGG
EcoT22I 810 ATGCAT ATGCA^T
1003 ATGCAT
MfeI 1301 CAATTG C^AATTG
1849 CAATTG
MunI 1301 CAATTG C^AATTG
1849 CAATTG
NaeI 2 GCCGGC GCC^GGC
131 GCCGGC
NgoMIV 2 GCCGGC G^CCGGC
131 GCCGGC
NsiI 810 ATGCAT ATGCA^T
1003 ATGCAT
PaeR7I 1630 CTCGAG C^TCGAG
3703 CTCGAG
PflFI 3034 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3622 GACCTGGTC
PflMI 3240 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4475 CCATTTTCTGG
SfcI 1446 CTGCAG C^T[GA][CT]AG
2546 CTATAG
StuI 3114 AGGCCT AGG^CCT
4186 AGGCCT
Tth111I 3034 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
3622 GACCTGGTC
Van91I 3240 CCAAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4475 CCATTTTCTGG
XhoI 1630 CTCGAG C^TCGAG
3703 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AseI AsnI
Asp700I AvaI AvaII BbsI BbvI
BclI BcnI BfaI BmyI BpmI
BpuAI BsaBI BsaHI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraI
DraII EarI EcoO109I EcoRI EcoRII
EcoT14I FauI FbaI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PshAI
PshBI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SmlI SspI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XbaI
XcmI XhoII XmnI