##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
AlwNI Aor51HI AscI AspEI AspI
AviII AvrII BamHI BbrPI BcgI
BclI BglII BlnI BsaBI BsaWI
BseAI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Cfr10I ClaI CpoI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI FbaI FseI FspI
HgaI HincII HindII HpaI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SwaI
Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 1243 GGTACC G^GTACC
AccI 2654 GTAGAC GT^[AC][GT]AC
AcyI 85 GGCGCC G[GA]^CG[CT]C
AflII 2528 CTTAAG C^TTAAG
Alw44I 1850 GTGCAC G^TGCAC
ApaI 1395 GGGCCC GGGCC^C
ApaLI 1850 GTGCAC G^TGCAC
AseI 2060 ATTAAT AT^TAAT
AsnI 2060 ATTAAT AT^TAAT
Asp700I 1424 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1243 GGTACC G^GTACC
BbeI 85 GGCGCC GGCGC^C
BciVI 2021 GTATCC GTATCC(6/5)
BfrI 2528 CTTAAG C^TTAAG
BlpI 585 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 585 GCTCAGC GC^T[ACGT]AGC
BsaAI 1760 CACGTA [CT]AC^GT[GA]
BsaHI 85 GGCGCC G[GA]^CG[CT]C
BsgI 1281 GTGCAG GTGCAG(16/14)
BspHI 938 TCATGA T^CATGA
BsrDI 2665 GCAATG GCAATG(2/0)
BssSI 248 CACGAG CACGAG(-5/-1)
CelII 585 GCTCAGC GC^T[ACGT]AGC
DraI 2186 TTTAAA TTT^AAA
DrdI 149 GACTCCTAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 2470 GATATC GAT^ATC
HaeII 85 GGCGCC [GA]GCGC^[CT]
Hin1I 85 GGCGCC G[GA]^CG[CT]C
HindIII 1141 AAGCTT A^AGCTT
KasI 85 GGCGCC G^GCGCC
KpnI 1243 GGTACC GGTAC^C
KspI 899 CCGCGG CCGC^GG
NarI 85 GGCGCC GG^CGCC
NheI 1466 GCTAGC G^CTAGC
PshBI 2060 ATTAAT AT^TAAT
PvuII 369 CAGCTG CAG^CTG
RcaI 938 TCATGA T^CATGA
SacII 899 CCGCGG CCGC^GG
SfiI 353 GGCCGTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 85 GGCGCC GGC^GCC
SphI 674 GCATGC GCATG^C
SspI 2063 AATATT AAT^ATT
XcmI 1059 CCAGCCTCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1424 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1255 GAATTT [GA]^AATT[CT]
1725 AAATTC
AflIII 79 ACATGT A^C[GA][CT]GT
2969 ACATGT
AlwI 383 GATCC GGATC(4/5)
2219 GATCC
AocI 548 CCTCAGG CC^T[ACGT]AGG
630 CCTCAGG
ApoI 1255 GAATTT [GA]^AATT[CT]
1725 AAATTC
BalI 566 TGGCCA TGG^CCA
2601 TGGCCA
BspLU11I 79 ACATGT A^CATGT
2969 ACATGT
BspMI 2535 ACCTGC ACCTGC(4/8)
1240 GCAGGT
BstYI 382 AGATCC [GA]^GATC[CT]
2218 AGATCC
Bsu36I 548 CCTCAGG CC^T[ACGT]AGG
630 CCTCAGG
Eco81I 548 CCTCAGG CC^T[ACGT]AGG
630 CCTCAGG
EcoT22I 1825 ATGCAT ATGCA^T
1862 ATGCAT
MaeII 1761 ACGT A^CGT
2202 ACGT
MflI 382 AGATCC [GA]^GATC[CT]
2218 AGATCC
MluNI 566 TGGCCA TGG^CCA
2601 TGGCCA
MscI 566 TGGCCA TGG^CCA
2601 TGGCCA
NsiI 1825 ATGCAT ATGCA^T
1862 ATGCAT
PaeR7I 1496 CTCGAG C^TCGAG
2296 CTCGAG
PshAI 1420 GACTGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2574 GACCCAGGTC
SapI 111 GCTCTTC GCTCTTC(1/4)
794 GCTCTTC
SmaI 92 CCCGGG CCC^GGG
1391 CCCGGG
StuI 1271 AGGCCT AGG^CCT
1300 AGGCCT
TfiI 1289 GAATC G^A[AT]TC
2179 GAATC
XhoI 1496 CTCGAG C^TCGAG
2296 CTCGAG
XhoII 382 AGATCC [GA]^GATC[CT]
2218 AGATCC
XmaCI 92 CCCGGG C^CCGGG
1391 CCCGGG
XmaI 92 CCCGGG C^CCGGG
1391 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I