##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AflII AgeI AscI AseI
AsnI Asp718I BbrPI BclI BfrI
BsiWI Bsp1407I BspDI BspHI BsrGI
BssHII Bst1107I BstBI BstZ17I ClaI
DrdI EcoRI FbaI FseI HpaI
KpnI MfeI MluI MunI NdeI
NheI NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI RcaI SalI SbfI SexAI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 168 GTCTAC GT^[AC][GT]AC
AcsI 287 GAATTT [GA]^AATT[CT]
AhdI 2691 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2486 GTGCAC G^TGCAC
AocI 3482 CCTTAGG CC^T[ACGT]AGG
Aor51HI 145 AGCGCT AGC^GCT
ApaI 3581 GGGCCC GGGCC^C
ApaLI 2486 GTGCAC G^TGCAC
ApoI 287 GAATTT [GA]^AATT[CT]
AspEI 2691 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 3818 TGCGCA TGC^GCA
AvrII 1482 CCTAGG C^CTAGG
BalI 3854 TGGCCA TGG^CCA
BamHI 4502 GGATCC G^GATCC
BbvCI 1927 CCTCAGC CCTCAGC(-5/-2)
BcgI 23 GCAGCGGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2246 GGATAC GTATCC(6/5)
BglII 3260 AGATCT A^GATCT
BlnI 1482 CCTAGG C^CTAGG
BsaBI 270 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 620 CGGCCG CG[GA][CT]^CG
BssSI 1128 CTCGTG CACGAG(-5/-1)
BstAPI 1628 GCAGGATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3673 GGTGACC G^GT[ACGT]ACC
BstPI 3673 GGTGACC G^GT[ACGT]ACC
BstXI 4616 CCAAGGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3482 CCTTAGG CC^T[ACGT]AGG
CpoI 624 CGGACCG CG^G[AT]CCG
EagI 620 CGGCCG C^GGCCG
Eam1105I 2691 GACTAAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 620 CGGCCG C^GGCCG
Eco47III 145 AGCGCT AGC^GCT
Eco52I 620 CGGCCG C^GGCCG
Eco81I 3482 CCTTAGG CC^T[ACGT]AGG
EcoNI 711 CCTCCCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 3673 GGTGACC G^GT[ACGT]ACC
EcoRV 1435 GATATC GAT^ATC
FspI 3818 TGCGCA TGC^GCA
MamI 270 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3854 TGGCCA TGG^CCA
MscI 3854 TGGCCA TGG^CCA
NaeI 129 GCCGGC GCC^GGC
NcoI 1249 CCATGG C^CATGG
NgoMIV 129 GCCGGC G^CCGGC
PaeR7I 1197 CTCGAG C^TCGAG
PshAI 2473 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 624 CGGACCG CG^G[AT]CCG
SacI 1194 GAGCTC GAGCT^C
SapI 704 GAAGAGC GCTCTTC(1/4)
ScaI 3151 AGTACT AGT^ACT
SfiI 4459 GGCCATGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SwaI 4941 ATTTAAAT ATTT^AAAT
XcmI 3585 CCAGGCTGGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1197 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 564 GACGTC GACGT^C
2417 GACGTC
AccIII 1291 TCCGGA T^CCGGA
1336 TCCGGA
AclI 3519 AACGTT AA^CGTT
4640 AACGTT
AflIII 1384 ACATGT A^C[GA][CT]GT
2745 ACATGT
Asp700I 4220 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4405 GAAGTATTTC
AspI 3072 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4955 GACAATGTC
BbeI 2519 GGCGCC GGCGC^C
4909 GGCGCC
BseAI 1291 TCCGGA T^CCGGA
1336 TCCGGA
BspEI 1291 TCCGGA T^CCGGA
1336 TCCGGA
BspLU11I 1384 ACATGT A^CATGT
2745 ACATGT
HincII 4916 GTTGAC GT[CT]^[GA]AC
4969 GTTGAC
HindII 4916 GTTGAC GT[CT]^[GA]AC
4969 GTTGAC
KasI 2519 GGCGCC G^GCGCC
4909 GGCGCC
KspI 113 CCGCGG CCGC^GG
3569 CCGCGG
MroI 1291 TCCGGA T^CCGGA
1336 TCCGGA
NarI 2519 GGCGCC GG^CGCC
4909 GGCGCC
PflFI 3072 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4955 GACAATGTC
PflMI 1843 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1879 CCAAGCCCTGG
Psp1406I 3519 AACGTT AA^CGTT
4640 AACGTT
SacII 113 CCGCGG CCGC^GG
3569 CCGCGG
SfoI 2519 GGCGCC GGC^GCC
4909 GGCGCC
TaqI 1198 TCGA T^CGA
4403 TCGA
Tth111I 3072 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4955 GACAATGTC
TthHB8I 1198 TCGA T^CGA
4403 TCGA
Van91I 1843 CCATCAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1879 CCAAGCCCTGG
XbaI 893 TCTAGA T^CTAGA
2315 TCTAGA
XmnI 4220 GAAACCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4405 GAAGTATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CelII
CfoI Cfr10I Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NsiI
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII