##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AocI Aor51HI AscI Asp718I AspI
AvrII BbeI BbrPI BfrI BlnI
BsaI BseAI BsiWI BspDI BspEI
BsrFI BssHII BssSI Bsu36I Cfr10I
ClaI CpoI Eco47III Eco81I EcoNI
EcoT14I FseI KasI KpnI MluI
MroI NaeI NarI NcoI NgoMIV
NheI NotI NruI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PvuI RsrII SacI SalI
SapI SbfI SexAI SfoI SgrAI
SnaBI SpeI Sse8387I StuI StyI
Tth111I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccI 3649 GTATAC GT^[AC][GT]AC
AclI 1820 AACGTT AA^CGTT
AcyI 2436 GGCGTC G[GA]^CG[CT]C
AflIII 2904 ACATGT A^C[GA][CT]GT
AhdI 1620 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 4269 GGGCCC GGGCC^C
AspEI 1620 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 967 TGCGCA TGC^GCA
BalI 1914 TGGCCA TGG^CCA
BanII 4269 GGGCCC G[GA]GC[CT]^C
BcgI 203 CGAGTATGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2300 GTATCC GTATCC(6/5)
BclI 4305 TGATCA T^GATCA
BglI 97 GCCTCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 4559 TACGTG [CT]AC^GT[GA]
BsaBI 2258 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2436 GGCGTC G[GA]^CG[CT]C
BsaWI 1699 ACCGGA [AT]^CCGG[AT]
BseRI 2556 CTCCTC GAGGAG(10/8)
BsmBI 1229 CGTCTC CGTCTC(1/5)
Bsp1407I 3531 TGTACA T^GTACA
BspLU11I 2904 ACATGT A^CATGT
BspMI 1127 GCAGGT ACCTGC(4/8)
BsrBI 54 CCGCTC CCGCTC(-3/-3)
BsrGI 3531 TGTACA T^GTACA
Bst1107I 3649 GTATAC GTA^TAC
BstEII 1519 GGTGACC G^GT[ACGT]ACC
BstPI 1519 GGTGACC G^GT[ACGT]ACC
BstZ17I 3649 GTATAC GTA^TAC
EagI 115 CGGCCG C^GGCCG
Eam1105I 1620 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 115 CGGCCG C^GGCCG
Eco52I 115 CGGCCG C^GGCCG
EcoO65I 1519 GGTGACC G^GT[ACGT]ACC
EcoRV 1451 GATATC GAT^ATC
FbaI 4305 TGATCA T^GATCA
FspI 967 TGCGCA TGC^GCA
HaeII 134 GGCGCT [GA]GCGC^[CT]
Hin1I 2436 GGCGTC G[GA]^CG[CT]C
HpaI 5008 GTTAAC GTT^AAC
MamI 2258 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2394 CAATTG C^AATTG
MluNI 1914 TGGCCA TGG^CCA
MscI 1914 TGGCCA TGG^CCA
MunI 2394 CAATTG C^AATTG
PacI 3457 TTAATTAA TTAAT^TAA
Psp1406I 1820 AACGTT AA^CGTT
PstI 4831 CTGCAG CTGCA^G
ScaI 2836 AGTACT AGT^ACT
SfiI 96 GGCCTCCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 1116 CCCGGG CCC^GGG
SmlI 3339 CTTGAG C^T[CT][GA]AG
SspBI 3531 TGTACA T^GTACA
XbaI 2273 TCTAGA T^CTAGA
XmaCI 1116 CCCGGG C^CCGGG
XmaI 1116 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Alw44I 3763 GTGCAC G^TGCAC
4846 GTGCAC
ApaLI 3763 GTGCAC G^TGCAC
4846 GTGCAC
AseI 3456 ATTAAT AT^TAAT
4648 ATTAAT
AsnI 3456 ATTAAT AT^TAAT
4648 ATTAAT
AvaI 201 CCCGAG C^[CT]CG[GA]G
1116 CCCGGG
BamHI 994 GGATCC G^GATCC
1576 GGATCC
BbvCI 764 CCTCAGC CCTCAGC(-5/-2)
2674 CCTCAGC
BglII 1505 AGATCT A^GATCT
2641 AGATCT
BlpI 154 GCTGAGC GC^T[ACGT]AGC
2714 GCTGAGC
Bpu1102I 154 GCTGAGC GC^T[ACGT]AGC
2714 GCTGAGC
BsiEI 115 CGGCCG CG[GA][CT]^CG
180 CGGTCG
BsoBI 201 CCCGAG C^[CT]CG[GA]G
1116 CCCGGG
BsrDI 1162 CATTGC GCAATG(2/0)
1918 CATTGC
BstBI 1244 TTCGAA TT^CGAA
2073 TTCGAA
CelII 154 GCTGAGC GC^T[ACGT]AGC
2714 GCTGAGC
DrdI 37 GACCGCGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4237 GACAGTGGAGTC
DsaI 39 CCGCGG C^C[GA][CT]GG
101 CCGCGG
EaeI 115 CGGCCG [CT]^GGCC[GA]
1914 TGGCCA
EarI 872 GAAGAG CTCTTC(1/4)
3523 GAAGAG
EcoT22I 1640 ATGCAT ATGCA^T
4614 ATGCAT
Ksp632I 872 GAAGAG CTCTTC(1/4)
3523 GAAGAG
KspI 39 CCGCGG CCGC^GG
101 CCGCGG
NsiI 1640 ATGCAT ATGCA^T
4614 ATGCAT
NspV 1244 TTCGAA TT^CGAA
2073 TTCGAA
PshBI 3456 ATTAAT AT^TAAT
4648 ATTAAT
PvuII 1778 CAGCTG CAG^CTG
4280 CAGCTG
SacII 39 CCGCGG CCGC^GG
101 CCGCGG
SfaNI 1094 GATGC GCATC(5/9)
1109 GATGC
SfuI 1244 TTCGAA TT^CGAA
2073 TTCGAA
SphI 2127 GCATGC GCATG^C
2596 GCATGC
SwaI 3087 ATTTAAAT ATTT^AAAT
5014 ATTTAAAT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaII BanI BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspHI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DraIII
EcoO109I EcoRI EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HincII HindII HindIII HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PflMI PleI
RcaI RsaI Sau3AI Sau96I ScrFI
SfcI SspI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI