##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AscI BbeI BbrPI BbvCI BfrI
BsaAI BsiWI BspDI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EcoNI EcoO65I EcoRI
EcoT22I FseI HpaI KasI KspI
MluI NarI NdeI NheI NotI
NruI NsiI NspV PinAI PmaCI
PmeI PmlI PshAI Psp1406I RsrII
SacII SalI SapI SexAI SfoI
SfuI SgrAI SnaBI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2516 GGTACC G^GTACC
AhdI 1613 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 6079 CCTCAGG CC^T[ACGT]AGG
Aor51HI 561 AGCGCT AGC^GCT
Asp718I 2516 GGTACC G^GTACC
AspEI 1613 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 3767 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 3096 TGCGCA TGC^GCA
AvrII 2038 CCTAGG C^CTAGG
BalI 1557 TGGCCA TGG^CCA
BcgI 4731 GCAGGGAGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2934 GTATCC GTATCC(6/5)
BclI 2016 TGATCA T^GATCA
BlnI 2038 CCTAGG C^CTAGG
BsaBI 2662 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1855 GAATGC GAATGC(1/-1)
BspLU11I 3857 ACATGT A^CATGT
BsrFI 5036 GCCGGC [GA]^CCGG[CT]
Bsu36I 6079 CCTCAGG CC^T[ACGT]AGG
Cfr10I 5036 GCCGGC [GA]^CCGG[CT]
EagI 1748 CGGCCG C^GGCCG
Eam1105I 1613 GACGATGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1748 CGGCCG C^GGCCG
Eco47III 561 AGCGCT AGC^GCT
Eco52I 1748 CGGCCG C^GGCCG
Eco81I 6079 CCTCAGG CC^T[ACGT]AGG
EcoRV 2278 GATATC GAT^ATC
FbaI 2016 TGATCA T^GATCA
FspI 3096 TGCGCA TGC^GCA
HincII 284 GTCAAC GT[CT]^[GA]AC
HindII 284 GTCAAC GT[CT]^[GA]AC
HindIII 179 AAGCTT A^AGCTT
KpnI 2516 GGTACC GGTAC^C
MamI 2662 GATCTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 445 CAATTG C^AATTG
MluNI 1557 TGGCCA TGG^CCA
MscI 1557 TGGCCA TGG^CCA
MunI 445 CAATTG C^AATTG
NaeI 5036 GCCGGC GCC^GGC
NgoMIV 5036 GCCGGC G^CCGGC
PacI 2177 TTAATTAA TTAAT^TAA
PflFI 3767 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 612 CGATCG CGAT^CG
SbfI 736 CCTGCAGG CCTGCA^GG
ScaI 1195 AGTACT AGT^ACT
SfiI 5600 GGCCCTGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 3964 CCCGGG CCC^GGG
SpeI 4707 ACTAGT A^CTAGT
Sse8387I 736 CCTGCAGG CCTGCA^GG
Tth111I 3767 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3964 CCCGGG C^CCGGG
XmaI 3964 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 1068 TCCGGA T^CCGGA
4350 TCCGGA
AflIII 3857 ACATGT A^C[GA][CT]GT
5347 ACGTGT
Alw44I 697 GTGCAC G^TGCAC
5213 GTGCAC
ApaLI 697 GTGCAC G^TGCAC
5213 GTGCAC
AseI 2180 ATTAAT AT^TAAT
5799 ATTAAT
AsnI 2180 ATTAAT AT^TAAT
5799 ATTAAT
BanI 2516 GGTACC G^G[CT][GA]CC
4720 GGTGCC
BglII 1242 AGATCT A^GATCT
2661 AGATCT
BsaI 1716 GAGACC GGTCTC(1/5)
3790 GGTCTC
BseAI 1068 TCCGGA T^CCGGA
4350 TCCGGA
Bsp1407I 6163 TGTACA T^GTACA
6187 TGTACA
BspEI 1068 TCCGGA T^CCGGA
4350 TCCGGA
BspHI 1014 TCATGA T^CATGA
2241 TCATGA
BsrGI 6163 TGTACA T^GTACA
6187 TGTACA
DraI 3320 TTTAAA TTT^AAA
5793 TTTAAA
MroI 1068 TCCGGA T^CCGGA
4350 TCCGGA
NcoI 768 CCATGG C^CATGG
3009 CCATGG
PaeR7I 1767 CTCGAG C^TCGAG
2610 CTCGAG
PshBI 2180 ATTAAT AT^TAAT
5799 ATTAAT
RcaI 1014 TCATGA T^CATGA
2241 TCATGA
SphI 2592 GCATGC GCATG^C
5340 GCATGC
SspBI 6163 TGTACA T^GTACA
6187 TGTACA
XcmI 3686 CCACTGAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3775 CCACCAGAGCTCTGG
XhoI 1767 CTCGAG C^TCGAG
2610 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BamHI
BanII BbsI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaJI BsaWI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrDI BsrI BssKI
BssSI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XhoII
XmnI