##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII AccI AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AviII AvrII BamHI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrGI BssHII Bst1107I
BstBI BstXI BstZ17I CelII ClaI
CpoI DraI DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI MamI
MfeI MluI MslI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SexAI SfiI
SfuI SnaBI SpeI SphI SspBI
SspI SwaI XbaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1939 GGTACC G^GTACC
AcyI 2446 GGCGCC G[GA]^CG[CT]C
Alw44I 1597 GTGCAC G^TGCAC
ApaLI 1597 GTGCAC G^TGCAC
Asp700I 169 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1939 GGTACC G^GTACC
AspI 1081 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1340 GGACC G^G[AT]CC
BalI 1438 TGGCCA TGG^CCA
BbeI 2446 GGCGCC GGCGC^C
BbrPI 634 CACGTG CAC^GTG
BbvCI 2586 GCTGAGG CCTCAGC(-5/-2)
BcgI 2370 CGAGAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaAI 634 CACGTG [CT]AC^GT[GA]
BsaHI 2446 GGCGCC G[GA]^CG[CT]C
BsmI 64 GAATGC GAATGC(1/-1)
BsrBI 2526 CCGCTC CCGCTC(-3/-3)
BsrDI 2784 GCAATG GCAATG(2/0)
BsrFI 2599 GCCGGT [GA]^CCGG[CT]
BssSI 1434 CTCGTG CACGAG(-5/-1)
BstAPI 646 GCATCGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 974 GGTCACC G^GT[ACGT]ACC
BstPI 974 GGTCACC G^GT[ACGT]ACC
Cfr10I 2599 GCCGGT [GA]^CCGG[CT]
DraII 2737 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1722 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2634 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2737 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 974 GGTCACC G^GT[ACGT]ACC
HaeII 2446 GGCGCC [GA]GCGC^[CT]
HgaI 2179 GACGC GACGC(5/10)
Hin1I 2446 GGCGCC G[GA]^CG[CT]C
HpaI 2836 GTTAAC GTT^AAC
KasI 2446 GGCGCC G^GCGCC
KpnI 1939 GGTACC GGTAC^C
KspI 15 CCGCGG CCGC^GG
MluNI 1438 TGGCCA TGG^CCA
MscI 1438 TGGCCA TGG^CCA
NarI 2446 GGCGCC GG^CGCC
NcoI 1344 CCATGG C^CATGG
PaeR7I 1550 CTCGAG C^TCGAG
PflFI 1081 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 634 CACGTG CAC^GTG
PmlI 634 CACGTG CAC^GTG
SacI 312 GAGCTC GAGCT^C
SacII 15 CCGCGG CCGC^GG
SbfI 2505 CCTGCAGG CCTGCA^GG
SfoI 2446 GGCGCC GGC^GCC
SgrAI 2598 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2471 CCCGGG CCC^GGG
Sse8387I 2505 CCTGCAGG CCTGCA^GG
StuI 1012 AGGCCT AGG^CCT
Tth111I 1081 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2081 CCAAAACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1550 CTCGAG C^TCGAG
XmaCI 2471 CCCGGG C^CCGGG
XmaI 2471 CCCGGG C^CCGGG
XmnI 169 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccIII 1088 TCCGGA T^CCGGA
1215 TCCGGA
AflII 1908 CTTAAG C^TTAAG
3008 CTTAAG
AflIII 2402 ACATGT A^C[GA][CT]GT
3110 ACATGT
AlwNI 1442 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2767 CAGGATCTG
AocI 1062 CCTCAGG CC^T[ACGT]AGG
1389 CCTGAGG
BfrI 1908 CTTAAG C^TTAAG
3008 CTTAAG
BglII 829 AGATCT A^GATCT
2018 AGATCT
BsaWI 1088 TCCGGA [AT]^CCGG[AT]
1215 TCCGGA
BseAI 1088 TCCGGA T^CCGGA
1215 TCCGGA
BspEI 1088 TCCGGA T^CCGGA
1215 TCCGGA
BspLU11I 2402 ACATGT A^CATGT
3110 ACATGT
Bsu36I 1062 CCTCAGG CC^T[ACGT]AGG
1389 CCTGAGG
EaeI 1438 TGGCCA [CT]^GGCC[GA]
1758 TGGCCG
Eco81I 1062 CCTCAGG CC^T[ACGT]AGG
1389 CCTGAGG
HincII 2326 GTCAAC GT[CT]^[GA]AC
2836 GTTAAC
HindII 2326 GTCAAC GT[CT]^[GA]AC
2836 GTTAAC
HindIII 775 AAGCTT A^AGCTT
1879 AAGCTT
MaeII 635 ACGT A^CGT
2856 ACGT
MroI 1088 TCCGGA T^CCGGA
1215 TCCGGA
NspI 2402 ACATGT [GA]CATG^[CT]
3110 ACATGT
PflMI 996 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1441 CCAGGAGCTGG
PshAI 2034 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2191 GACCAGGGTC
SapI 104 GAAGAGC GCTCTTC(1/4)
3019 GCTCTTC
Van91I 996 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1441 CCAGGAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaI BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DsaI EarI EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII