##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI AscI AseI AsnI Asp700I
AspEI AvrII BciVI BclI BfrI
BglII BlnI BsaBI BseAI BseRI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspHI BsrGI Bst1107I BstBI BstEII
BstPI BstZ17I ClaI Eam1105I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI HincII HindII HindIII HpaI
MamI MfeI MluI MroI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PmeI PshAI
PshBI Psp1406I PvuI RcaI SalI
SexAI SfiI SfuI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 2815 ACATGT A^C[GA][CT]GT
AgeI 1981 ACCGGT A^CCGGT
Alw44I 710 GTGCAC G^TGCAC
Aor51HI 982 AGCGCT AGC^GCT
ApaLI 710 GTGCAC G^TGCAC
AspI 1107 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 2071 CGATCTACATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaWI 1981 ACCGGT [AT]^CCGG[AT]
BspLU11I 2815 ACATGT A^CATGT
BsrDI 3345 GCAATG GCAATG(2/0)
BssSI 3079 CTCGTG CACGAG(-5/-1)
BstAPI 2923 GCAAGCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 2329 CGGTCCG CG^G[AT]CCG
DraI 3155 TTTAAA TTT^AAA
DraIII 771 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 982 AGCGCT AGC^GCT
PflFI 1107 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1981 ACCGGT A^CCGGT
RsrII 2329 CGGTCCG CG^G[AT]CCG
SapI 1301 GCTCTTC GCTCTTC(1/4)
SbfI 2714 CCTGCAGG CCTGCA^GG
SfaNI 229 GATGC GCATC(5/9)
SgrAI 1980 CACCGGTG C[GA]^CCGG[CT]G
SmlI 1356 CTCAAG C^T[CT][GA]AG
Sse8387I 2714 CCTGCAGG CCTGCA^GG
Tth111I 1107 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
Acc65I 1035 GGTACC G^GTACC
1785 GGTACC
AocI 1701 CCTGAGG CC^T[ACGT]AGG
1881 CCTGAGG
Asp718I 1035 GGTACC G^GTACC
1785 GGTACC
AviII 126 TGCGCA TGC^GCA
783 TGCGCA
BamHI 2100 GGATCC G^GATCC
2408 GGATCC
BbeI 531 GGCGCC GGCGC^C
562 GGCGCC
BbvCI 1457 CCTCAGC CCTCAGC(-5/-2)
2959 CCTCAGC
BlpI 111 GCTGAGC GC^T[ACGT]AGC
2013 GCTGAGC
Bpu1102I 111 GCTGAGC GC^T[ACGT]AGC
2013 GCTGAGC
BsaI 2682 GAGACC GGTCTC(1/5)
1769 GGTCTC
BsgI 777 GTGCAG GTGCAG(16/14)
2897 GTGCAG
BsrBI 1836 CCGCTC CCGCTC(-3/-3)
2117 CCGCTC
BssHII 510 GCGCGC G^CGCGC
738 GCGCGC
Bsu36I 1701 CCTGAGG CC^T[ACGT]AGG
1881 CCTGAGG
CelII 111 GCTGAGC GC^T[ACGT]AGC
2013 GCTGAGC
DrdI 50 GACTCAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1500 GACCCGGAGGTC
EagI 617 CGGCCG C^GGCCG
633 CGGCCG
EclXI 617 CGGCCG C^GGCCG
633 CGGCCG
Eco52I 617 CGGCCG C^GGCCG
633 CGGCCG
Eco81I 1701 CCTGAGG CC^T[ACGT]AGG
1881 CCTGAGG
FspI 126 TGCGCA TGC^GCA
783 TGCGCA
KasI 531 GGCGCC G^GCGCC
562 GGCGCC
KpnI 1035 GGTACC GGTAC^C
1785 GGTACC
MslI 84 CACGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
789 CACAGCTGTG
NarI 531 GGCGCC GG^CGCC
562 GGCGCC
NcoI 1 CCATGG C^CATGG
1169 CCATGG
NspI 2077 ACATGC [GA]CATG^[CT]
2815 ACATGT
SacI 1824 GAGCTC GAGCT^C
2051 GAGCTC
ScaI 280 AGTACT AGT^ACT
3372 AGTACT
SfoI 531 GGCGCC GGC^GCC
562 GGCGCC
StuI 952 AGGCCT AGG^CCT
2671 AGGCCT
TaqI 227 TCGA T^CGA
1531 TCGA
TthHB8I 227 TCGA T^CGA
1531 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApaI ApoI
AspHI AvaI AvaII BalI BanI
BanII BbrPI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I KspI
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NaeI NciI NdeII NgoMIV NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI SacII
Sau3AI Sau96I ScrFI SfcI SmaI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XcmI XhoII
XmaCI XmaI