##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII AccI AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
AspEI BcgI BciVI BfrI BglII
BlpI Bpu1102I BsaBI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrGI
BssHII Bst1107I BstBI BstZ17I CelII
ClaI CpoI DraI DrdI Eam1105I
EcoRI EcoRV EcoT22I FseI HincII
HindII HindIII HpaI MamI MfeI
MluI MunI NdeI NheI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SalI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 83 GGTACC G^GTACC
AcsI 431 GAATTT [GA]^AATT[CT]
Alw44I 2086 GTGCAC G^TGCAC
Aor51HI 2046 AGCGCT AGC^GCT
ApaLI 2086 GTGCAC G^TGCAC
ApoI 431 GAATTT [GA]^AATT[CT]
Asp718I 83 GGTACC G^GTACC
AspI 1503 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2777 CCTAGG C^CTAGG
BamHI 1900 GGATCC G^GATCC
BbeI 2357 GGCGCC GGCGC^C
BbrPI 2225 CACGTG CAC^GTG
BbvCI 1408 CCTCAGC CCTCAGC(-5/-2)
BclI 1593 TGATCA T^GATCA
BlnI 2777 CCTAGG C^CTAGG
BsiEI 266 CGGCCG CG[GA][CT]^CG
BsmBI 1336 GAGACG CGTCTC(1/5)
BsrDI 66 GCAATG GCAATG(2/0)
BstAPI 1561 GCATGCCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1489 GGTCACC G^GT[ACGT]ACC
BstPI 1489 GGTCACC G^GT[ACGT]ACC
BstXI 1701 CCAGGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 2144 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 266 CGGCCG C^GGCCG
EclXI 266 CGGCCG C^GGCCG
Eco47III 2046 AGCGCT AGC^GCT
Eco52I 266 CGGCCG C^GGCCG
EcoO65I 1489 GGTCACC G^GT[ACGT]ACC
FbaI 1593 TGATCA T^GATCA
KasI 2357 GGCGCC G^GCGCC
KpnI 83 GGTACC GGTAC^C
KspI 2353 CCGCGG CCGC^GG
MseI 369 TTAA T^TAA
NarI 2357 GGCGCC GG^CGCC
NcoI 184 CCATGG C^CATGG
PaeR7I 508 CTCGAG C^TCGAG
PflFI 1503 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 698 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2225 CACGTG CAC^GTG
PmlI 2225 CACGTG CAC^GTG
SacII 2353 CCGCGG CCGC^GG
SbfI 1904 CCTGCAGG CCTGCA^GG
ScaI 2001 AGTACT AGT^ACT
SexAI 2090 ACCAGGT A^CC[AT]GGT
SfoI 2357 GGCGCC GGC^GCC
SphI 1561 GCATGC GCATG^C
Sse8387I 1904 CCTGCAGG CCTGCA^GG
TfiI 220 GAATC G^A[AT]TC
Tru9I 369 TTAA T^TAA
Tsp509I 432 AATT ^AATT
Tth111I 1503 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 698 CCACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 508 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccIII 906 TCCGGA T^CCGGA
1332 TCCGGA
AflIII 2226 ACGTGT A^C[GA][CT]GT
2232 ACGTGT
AviII 1884 TGCGCA TGC^GCA
3006 TGCGCA
BfaI 1224 CTAG C^TAG
2778 CTAG
BsaAI 2225 CACGTG [CT]AC^GT[GA]
2231 TACGTG
BsaI 1286 GAGACC GGTCTC(1/5)
2458 GGTCTC
BsaWI 906 TCCGGA [AT]^CCGG[AT]
1332 TCCGGA
BseAI 906 TCCGGA T^CCGGA
1332 TCCGGA
BspEI 906 TCCGGA T^CCGGA
1332 TCCGGA
BsrBI 687 CCGCTC CCGCTC(-3/-3)
1292 CCGCTC
BssSI 2651 CACGAG CACGAG(-5/-1)
215 CTCGTG
FspI 1884 TGCGCA TGC^GCA
3006 TGCGCA
MaeI 1224 CTAG C^TAG
2778 CTAG
MroI 906 TCCGGA T^CCGGA
1332 TCCGGA
PleI 2798 GACTC GAGTC(4/5)
946 GAGTC
SapI 1312 GAAGAGC GCTCTTC(1/4)
1441 GAAGAGC
SfiI 862 GGCCCGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2773 GGCCCCTAGGGCC
SmlI 320 CTTGAG C^T[CT][GA]AG
508 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 114 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI StuI StyI
TaqI TseI Tsp45I TspRI TthHB8I
XcmI XhoII XmaCI XmaI