##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AccIII AclI AgeI Aor51HI ApaI
AscI AspI AviII BbeI BbrPI
BbvCI BcgI BclI BglII BlpI
Bpu1102I BsaBI BseAI BsiWI Bsp1407I
BspDI BspEI BsrGI BssHII BssSI
BstBI BstEII BstPI CelII ClaI
CpoI Eco47III EcoO65I FbaI FseI
FspI HpaI KasI KspI MamI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SfoI
SfuI SnaBI SpeI SphI Sse8387I
SspBI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
AatII 1455 GACGTC GACGT^C
AcyI 1455 GACGTC G[GA]^CG[CT]C
AflIII 10 ACATGT A^C[GA][CT]GT
AhdI 3198 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 405 CCTGAGG CC^T[ACGT]AGG
AspEI 3198 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BciVI 1256 GGATAC GTATCC(6/5)
BsaAI 489 TACGTG [CT]AC^GT[GA]
BsaHI 1455 GACGTC G[GA]^CG[CT]C
BsaWI 5020 ACCGGA [AT]^CCGG[AT]
BsiEI 80 CGGCCG CG[GA][CT]^CG
BsmI 4679 GCATTC GAATGC(1/-1)
BspLU11I 10 ACATGT A^CATGT
BstAPI 5540 GCACCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 405 CCTGAGG CC^T[ACGT]AGG
DraIII 567 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3909 GACGCAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 80 CGGCCG C^GGCCG
Eam1105I 3198 GACTCAGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 80 CGGCCG C^GGCCG
Eco52I 80 CGGCCG C^GGCCG
Eco81I 405 CCTGAGG CC^T[ACGT]AGG
EcoRV 588 GATATC GAT^ATC
EcoT22I 6108 ATGCAT ATGCA^T
Hin1I 1455 GACGTC G[GA]^CG[CT]C
HincII 282 GTCAAC GT[CT]^[GA]AC
HindII 282 GTCAAC GT[CT]^[GA]AC
NcoI 4725 CCATGG C^CATGG
NsiI 6108 ATGCAT ATGCA^T
ScaI 6003 AGTACT AGT^ACT
SgrAI 196 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 213 CCCGGG CCC^GGG
SspI 4834 AATATT AAT^ATT
SwaI 5194 ATTTAAAT ATTT^AAAT
XmaCI 213 CCCGGG C^CCGGG
XmaI 213 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 1923 GGTACC G^GTACC
5253 GGTACC
AccII 242 CGCG CG^CG
287 CGCG
AflII 2108 CTTAAG C^TTAAG
5839 CTTAAG
Alw44I 312 GTGCAC G^TGCAC
745 GTGCAC
ApaLI 312 GTGCAC G^TGCAC
745 GTGCAC
AseI 1093 ATTAAT AT^TAAT
1579 ATTAAT
AsnI 1093 ATTAAT AT^TAAT
1579 ATTAAT
Asp718I 1923 GGTACC G^GTACC
5253 GGTACC
AvrII 4607 CCTAGG C^CTAGG
4864 CCTAGG
BalI 563 TGGCCA TGG^CCA
2499 TGGCCA
BfrI 2108 CTTAAG C^TTAAG
5839 CTTAAG
BlnI 4607 CCTAGG C^CTAGG
4864 CCTAGG
BsgI 3709 CTGCAC GTGCAG(16/14)
888 GTGCAG
BsmBI 1397 GAGACG CGTCTC(1/5)
4895 GAGACG
BspHI 3497 TCATGA T^CATGA
4132 TCATGA
BsrBI 33 GAGCGG CCGCTC(-3/-3)
432 GAGCGG
BsrDI 1621 CATTGC GCAATG(2/0)
6287 CATTGC
Bst1107I 1303 GTATAC GTA^TAC
2902 GTATAC
BstUI 242 CGCG CG^CG
287 CGCG
BstZ17I 1303 GTATAC GTA^TAC
2902 GTATAC
DraI 4921 TTTAAA TTT^AAA
5195 TTTAAA
EcoRI 2380 GAATTC G^AATTC
2444 GAATTC
HgaI 3909 GACGC GACGC(5/10)
268 GCGTC
KpnI 1923 GGTACC GGTAC^C
5253 GGTACC
MluNI 563 TGGCCA TGG^CCA
2499 TGGCCA
MscI 563 TGGCCA TGG^CCA
2499 TGGCCA
MvnI 242 CGCG CG^CG
287 CGCG
PshBI 1093 ATTAAT AT^TAAT
1579 ATTAAT
RcaI 3497 TCATGA T^CATGA
4132 TCATGA
SexAI 1184 ACCTGGT A^CC[AT]GGT
6152 ACCTGGT
SfiI 227 GGCCGGCGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
348 GGCCGGGTGGGCC
StuI 3770 AGGCCT AGG^CCT
4460 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI AvaII BamHI BanI BanII
BbsI BbvI BcnI BfaI BglI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PpuMI PshAI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XbaI XcmI
XhoII XmnI