##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AflIII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI Asp718I AspEI
AviII AvrII BalI BanI BbeI
BbrPI BbvCI BclI BcnI BfrI
BglI BlnI BlpI Bpu1102I BsaAI
BsaWI BseAI BsiEI BsiWI BsmFI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NciI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI RsrII SacII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SphI Sse8387I
SspBI SwaI XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
AccII 77 CGCG CG^CG
AclI 122 AACGTT AA^CGTT
AcyI 20 GGCGTC G[GA]^CG[CT]C
Alw44I 1389 GTGCAC G^TGCAC
ApaLI 1389 GTGCAC G^TGCAC
AseI 1948 ATTAAT AT^TAAT
AsnI 1948 ATTAAT AT^TAAT
Asp700I 2072 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 384 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1371 CCCGAG C^[CT]CG[GA]G
BamHI 920 GGATCC G^GATCC
BcgI 909 GCAGAGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2132 AGATCT A^GATCT
BsaBI 5 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 20 GGCGTC G[GA]^CG[CT]C
BsaI 2007 GGTCTC GGTCTC(1/5)
BsgI 685 GTGCAG GTGCAG(16/14)
BsmBI 2836 CGTCTC CGTCTC(1/5)
BsoBI 1371 CCCGAG C^[CT]CG[GA]G
BspHI 1452 TCATGA T^CATGA
BspMI 341 GCAGGT ACCTGC(4/8)
BsrDI 1302 CATTGC GCAATG(2/0)
BstAPI 678 GCAGTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 77 CGCG CG^CG
DraII 1228 AGGACCC [GA]G^G[ACGT]CC[CT]
DsaI 1210 CCATGG C^C[GA][CT]GG
EcoO109I 1228 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 485 GATATC GAT^ATC
EcoT14I 1210 CCATGG C^C[AT][AT]GG
FauI 364 CCCGC CCCGC(4/6)
HgaI 21 GCGTC GACGC(5/10)
Hin1I 20 GGCGTC G[GA]^CG[CT]C
HindIII 833 AAGCTT A^AGCTT
MamI 5 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 77 CGCG CG^CG
NcoI 1210 CCATGG C^CATGG
NspI 2386 GCATGT [GA]CATG^[CT]
PflFI 384 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2659 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1228 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1948 ATTAAT AT^TAAT
Psp1406I 122 AACGTT AA^CGTT
PstI 2736 CTGCAG CTGCA^G
RcaI 1452 TCATGA T^CATGA
SacI 2580 GAGCTC GAGCT^C
ScaI 966 AGTACT AGT^ACT
SmlI 1015 CTCAAG C^T[CT][GA]AG
SpeI 2483 ACTAGT A^CTAGT
SspI 2869 AATATT AAT^ATT
StuI 913 AGGCCT AGG^CCT
StyI 1210 CCATGG C^C[AT][AT]GG
Tth111I 384 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 2659 CCACTCTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1097 CCAAAGAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2072 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
BanII 1025 GGGCTC G[GA]GC[CT]^C
2580 GAGCTC
BbsI 748 GAAGAC GAAGAC(2/6)
539 GTCTTC
BpmI 2583 CTCCAG CTGGAG(16/14)
1108 CTGGAG
BpuAI 748 GAAGAC GAAGAC(2/6)
539 GTCTTC
BstYI 920 GGATCC [GA]^GATC[CT]
2132 AGATCT
HaeII 63 AGCGCC [GA]GCGC^[CT]
2797 AGCGCC
HaeIII 914 GGCC GG^CC
2704 GGCC
HapII 305 CCGG C^CGG
1054 CCGG
HpaII 305 CCGG C^CGG
1054 CCGG
MflI 920 GGATCC [GA]^GATC[CT]
2132 AGATCT
MspI 305 CCGG C^CGG
1054 CCGG
PvuII 880 CAGCTG CAG^CTG
1105 CAGCTG
SapI 452 GCTCTTC GCTCTTC(1/4)
1027 GCTCTTC
TaqI 1010 TCGA T^CGA
1205 TCGA
TthHB8I 1010 TCGA T^CGA
1205 TCGA
XhoII 920 GGATCC [GA]^GATC[CT]
2132 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbvI BciVI
BfaI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraI EarI
EcoRII Fnu4HI FokI HhaI HinP1I
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MvaI MwoI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI