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# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AviII AvrII BalI
BbeI BbvCI BcgI BclI BglI
BlnI BsaHI BsaI BsaWI BseAI
BsiWI BsmBI BsmFI BsmI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
BssSI BstAPI BstBI Cfr10I ClaI
CpoI DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV FbaI FseI FspI
HaeII Hin1I KasI KspI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NgoMIV NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacII SalI
SbfI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SwaI
Van91I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 69 enzymes) #
###################################
Acc65I 641 GGTACC G^GTACC
AccI 1640 GTATAC GT^[AC][GT]AC
AccII 101 CGCG CG^CG
AflII 736 CTTAAG C^TTAAG
AflIII 1337 ACGTGT A^C[GA][CT]GT
AlwNI 1466 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2412 CCTAAGG CC^T[ACGT]AGG
Asp718I 641 GGTACC G^GTACC
AspI 3324 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1327 CTCGGG C^[CT]CG[GA]G
BamHI 979 GGATCC G^GATCC
BbrPI 1336 CACGTG CAC^GTG
BbsI 1581 GTCTTC GAAGAC(2/6)
BciVI 3383 GTATCC GTATCC(6/5)
BcnI 2447 CCGGG CC^[GC]GG
BfrI 736 CTTAAG C^TTAAG
BlpI 1237 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1237 GCTGAGC GC^T[ACGT]AGC
BpuAI 1581 GTCTTC GAAGAC(2/6)
BsaAI 1336 CACGTG [CT]AC^GT[GA]
BsaBI 1709 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 103 CGACCG CG[GA][CT]^CG
BsoBI 1327 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1861 TGTACA T^GTACA
BsrGI 1861 TGTACA T^GTACA
Bst1107I 1640 GTATAC GTA^TAC
BstEII 1125 GGTGACC G^GT[ACGT]ACC
BstPI 1125 GGTGACC G^GT[ACGT]ACC
BstUI 101 CGCG CG^CG
BstXI 19 CCATCTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1640 GTATAC GTA^TAC
Bsu36I 2412 CCTAAGG CC^T[ACGT]AGG
CelII 1237 GCTGAGC GC^T[ACGT]AGC
CfoI 1279 GCGC GCG^C
EarI 523 GAAGAG CTCTTC(1/4)
Eco81I 2412 CCTAAGG CC^T[ACGT]AGG
EcoO65I 1125 GGTGACC G^GT[ACGT]ACC
EcoT22I 1410 ATGCAT ATGCA^T
HapII 2447 CCGG C^CGG
HgaI 577 GACGC GACGC(5/10)
HhaI 1279 GCGC GCG^C
HinP1I 1279 GCGC G^CGC
HincII 1930 GTTAAC GT[CT]^[GA]AC
HindII 1930 GTTAAC GT[CT]^[GA]AC
HindIII 245 AAGCTT A^AGCTT
HpaI 1930 GTTAAC GTT^AAC
HpaII 2447 CCGG C^CGG
KpnI 641 GGTACC GGTAC^C
Ksp632I 523 GAAGAG CTCTTC(1/4)
MamI 1709 GATTTTTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 2447 CCGG C^CGG
MvnI 101 CGCG CG^CG
NciI 2447 CCGGG CC^[GC]GG
NdeI 3171 CATATG CA^TATG
NheI 955 GCTAGC G^CTAGC
NsiI 1410 ATGCAT ATGCA^T
PflFI 3324 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1336 CACGTG CAC^GTG
PmlI 1336 CACGTG CAC^GTG
PstI 588 CTGCAG CTGCA^G
PvuII 1420 CAGCTG CAG^CTG
SapI 523 GAAGAGC GCTCTTC(1/4)
SexAI 2674 ACCTGGT A^CC[AT]GGT
SphI 1250 GCATGC GCATG^C
SspBI 1861 TGTACA T^GTACA
SspI 784 AATATT AAT^ATT
StuI 2416 AGGCCT AGG^CCT
Tth111I 3324 GACATAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1717 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
Alw44I 1351 GTGCAC G^TGCAC
2690 GTGCAC
ApaLI 1351 GTGCAC G^TGCAC
2690 GTGCAC
Asp700I 1397 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1577 GAATGTCTTC
BsgI 2293 CTGCAC GTGCAG(16/14)
2536 GTGCAG
BsrBI 657 GAGCGG CCGCTC(-3/-3)
835 GAGCGG
BsrDI 798 CATTGC GCAATG(2/0)
1544 CATTGC
DraII 1133 AGGACCT [GA]G^G[ACGT]CC[CT]
3231 AGGACCC
DraIII 1333 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
1557 CACTCTGTG
EcoO109I 1133 AGGACCT [GA]G^G[ACGT]CC[CT]
3231 AGGACCC
EcoT14I 1438 CCTTGG C^C[AT][AT]GG
2419 CCTTGG
MaeII 1337 ACGT A^CGT
1532 ACGT
MspA1I 1420 CAGCTG C[AC]G^C[GT]G
2744 CAGCGG
PpuMI 1133 AGGACCT [GA]G^G[AT]CC[CT]
3231 AGGACCC
SacI 231 GAGCTC GAGCT^C
1240 GAGCTC
ScaI 1522 AGTACT AGT^ACT
3185 AGTACT
StyI 1438 CCTTGG C^C[AT][AT]GG
2419 CCTTGG
TaqI 409 TCGA T^CGA
2664 TCGA
TthHB8I 409 TCGA T^CGA
2664 TCGA
XmnI 1397 GAAAACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1577 GAATGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanI BanII
BbvI BfaI BglII BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII DraI EcoRII FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII