##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
ApaI AscI Asp700I AspEI AspI
AviII AvrII BbeI BbrPI BciVI
BfrI BglII BlnI BsaBI BsiEI
BsiWI Bsp1407I BspDI BsrBI BsrGI
BssSI BstBI BstEII BstPI ClaI
CpoI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRV FseI FspI
HincII HindII HpaI KasI MamI
MluI NaeI NarI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI Sse8387I SspBI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 362 GGTACC G^GTACC
AccIII 5234 TCCGGA T^CCGGA
Alw44I 1493 GTGCAC G^TGCAC
Aor51HI 896 AGCGCT AGC^GCT
ApaLI 1493 GTGCAC G^TGCAC
AseI 2448 ATTAAT AT^TAAT
AsnI 2448 ATTAAT AT^TAAT
Asp718I 362 GGTACC G^GTACC
BamHI 4869 GGATCC G^GATCC
BcgI 4436 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 509 TGATCA T^GATCA
BglI 20 GCCGTGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 2022 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2022 GCTCAGC GC^T[ACGT]AGC
BsaWI 5234 TCCGGA [AT]^CCGG[AT]
BseAI 5234 TCCGGA T^CCGGA
BspEI 5234 TCCGGA T^CCGGA
BspHI 4988 TCATGA T^CATGA
BspLU11I 5354 ACATGT A^CATGT
BsrFI 365 ACCGGC [GA]^CCGG[CT]
BssHII 941 GCGCGC G^CGCGC
Bst1107I 5358 GTATAC GTA^TAC
BstZ17I 5358 GTATAC GTA^TAC
CelII 2022 GCTCAGC GC^T[ACGT]AGC
Cfr10I 365 ACCGGC [GA]^CCGG[CT]
DrdI 4092 GACCTCTCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 896 AGCGCT AGC^GCT
EcoNI 4726 CCTGTGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 509 TGATCA T^GATCA
HaeII 896 AGCGCT [GA]GCGC^[CT]
KpnI 362 GGTACC GGTAC^C
KspI 118 CCGCGG CCGC^GG
MfeI 2478 CAATTG C^AATTG
MroI 5234 TCCGGA T^CCGGA
MunI 2478 CAATTG C^AATTG
PshAI 1377 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2448 ATTAAT AT^TAAT
RcaI 4988 TCATGA T^CATGA
SacII 118 CCGCGG CCGC^GG
SapI 2239 GAAGAGC GCTCTTC(1/4)
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AflIII 1954 ACGTGT A^C[GA][CT]GT
5354 ACATGT
BsaAI 3451 CACGTA [CT]AC^GT[GA]
4196 CACGTA
BsgI 745 GTGCAG GTGCAG(16/14)
1191 GTGCAG
BsrDI 7 CATTGC GCAATG(2/0)
2103 GCAATG
EcoRI 769 GAATTC G^AATTC
2809 GAATTC
EcoT22I 465 ATGCAT ATGCA^T
5335 ATGCAT
HindIII 4983 AAGCTT A^AGCTT
5015 AAGCTT
NcoI 2427 CCATGG C^CATGG
3966 CCATGG
NsiI 465 ATGCAT ATGCA^T
5335 ATGCAT
PflMI 286 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
714 CCAGGACGTGG
SacI 502 GAGCTC GAGCT^C
3330 GAGCTC
SphI 3544 GCATGC GCATG^C
5159 GCATGC
SspI 1947 AATATT AAT^ATT
2596 AATATT
TaqI 389 TCGA T^CGA
923 TCGA
TthHB8I 389 TCGA T^CGA
923 TCGA
Van91I 286 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
714 CCAGGACGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AlwNI AocI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XbaI XcmI
XhoII XmaCI XmaI