##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII Acc65I AccIII AclI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp718I AviII BbrPI BciVI BclI
BglII BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI BspEI BspHI BssHII
Bst1107I BstBI BstZ17I CelII DraI
Eco47III EcoRI EcoRV FbaI FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NaeI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 2105 AAATTC [GA]^AATT[CT]
AflII 4379 CTTAAG C^TTAAG
AgeI 1892 ACCGGT A^CCGGT
AhdI 125 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2105 AAATTC [GA]^AATT[CT]
Asp700I 4394 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 125 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 518 CCTAGG C^CTAGG
BcgI 494 GCAGCCCGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 4379 CTTAAG C^TTAAG
BlnI 518 CCTAGG C^CTAGG
BsaAI 3857 CACGTA [CT]AC^GT[GA]
BsaWI 1892 ACCGGT [AT]^CCGG[AT]
BsiEI 1361 CGGCCG CG[GA][CT]^CG
BspDI 3181 ATCGAT AT^CGAT
ClaI 3181 ATCGAT AT^CGAT
CpoI 141 CGGTCCG CG^G[AT]CCG
DrdI 4169 GACCAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1361 CGGCCG C^GGCCG
Eam1105I 125 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1361 CGGCCG C^GGCCG
Eco52I 1361 CGGCCG C^GGCCG
PinAI 1892 ACCGGT A^CCGGT
RsrII 141 CGGTCCG CG^G[AT]CCG
XmnI 4394 GAATCATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 576 GTCTAC GT^[AC][GT]AC
1835 GTAGAC
AspI 2227 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2352 GACTTTGTC
BbvCI 2887 CCTCAGC CCTCAGC(-5/-2)
3201 GCTGAGG
BfaI 519 CTAG C^TAG
4227 CTAG
BsmI 4130 GAATGC GAATGC(1/-1)
801 GCATTC
Bsp1407I 1974 TGTACA T^GTACA
4301 TGTACA
BspLU11I 993 ACATGT A^CATGT
2122 ACATGT
BsrDI 2578 CATTGC GCAATG(2/0)
2761 CATTGC
BsrGI 1974 TGTACA T^GTACA
4301 TGTACA
BstAPI 1667 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4143 GCAAGGCCTGC
BstEII 2411 GGTGACC G^GT[ACGT]ACC
4185 GGTCACC
BstPI 2411 GGTGACC G^GT[ACGT]ACC
4185 GGTCACC
DraIII 477 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
3334 CACACTGTG
EcoO65I 2411 GGTGACC G^GT[ACGT]ACC
4185 GGTCACC
EcoT22I 2052 ATGCAT ATGCA^T
3185 ATGCAT
HincII 999 GTTGAC GT[CT]^[GA]AC
4363 GTTGAC
HindII 999 GTTGAC GT[CT]^[GA]AC
4363 GTTGAC
KspI 36 CCGCGG CCGC^GG
435 CCGCGG
MaeI 519 CTAG C^TAG
4227 CTAG
NsiI 2052 ATGCAT ATGCA^T
3185 ATGCAT
PflFI 2227 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2352 GACTTTGTC
PflMI 1617 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1791 CCAGGGGGTGG
PpuMI 1150 AGGACCC [GA]G^G[AT]CC[CT]
4214 GGGTCCT
SacII 36 CCGCGG CCGC^GG
435 CCGCGG
SmaI 161 CCCGGG CCC^GGG
4180 CCCGGG
SspBI 1974 TGTACA T^GTACA
4301 TGTACA
TaqI 1321 TCGA T^CGA
3182 TCGA
Tth111I 2227 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2352 GACTTTGTC
TthHB8I 1321 TCGA T^CGA
3182 TCGA
Van91I 1617 CCAGCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1791 CCAGGGGGTGG
XmaCI 161 CCCGGG C^CCGGG
4180 CCCGGG
XmaI 161 CCCGGG C^CCGGG
4180 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BamHI
BanI BanII BbeI BbsI BbvI
BcnI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BssSI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
SphI StuI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XcmI
XhoII