##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AccIII AcyI AgeI
AhdI Alw44I AlwNI Aor51HI ApaI
ApaLI AscI Asp718I AspEI AspHI
AviII BalI BbeI BbrPI BbvCI
BcgI BciVI BclI BglI BlpI
Bpu1102I BsaHI BseAI BsgI BsiEI
BsiHKAI BsiWI Bsp1407I BspEI BsrGI
BssHII BstEII BstPI CelII CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I FauI FbaI FseI
FspI Hin1I KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NgoMIV NotI
PacI PflMI PinAI PmaCI PmeI
PmlI PvuI RsrII SacI SacII
SalI SbfI SexAI SfiI SfoI
SgrAI SmaI SpeI SphI Sse8387I
SspBI SwaI Van91I XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AocI 2236 CCTGAGG CC^T[ACGT]AGG
AspI 570 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2541 CTCGAG C^[CT]CG[GA]G
AvrII 5305 CCTAGG C^CTAGG
BamHI 2758 GGATCC G^GATCC
BanI 1906 GGCACC G^G[CT][GA]CC
BcnI 114 CCCGG CC^[GC]GG
BlnI 5305 CCTAGG C^CTAGG
BsoBI 2541 CTCGAG C^[CT]CG[GA]G
BspDI 848 ATCGAT AT^CGAT
BspLU11I 4561 ACATGT A^CATGT
BsrBI 2141 GAGCGG CCGCTC(-3/-3)
BsrFI 642 GCCGGT [GA]^CCGG[CT]
BstAPI 3043 GCAGAACTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2929 CCAAGGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2236 CCTGAGG CC^T[ACGT]AGG
Cfr10I 642 GCCGGT [GA]^CCGG[CT]
ClaI 848 ATCGAT AT^CGAT
DraII 5493 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 2075 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2964 CCATGG C^C[GA][CT]GG
EaeI 82 TGGCCG [CT]^GGCC[GA]
Eco81I 2236 CCTGAGG CC^T[ACGT]AGG
EcoO109I 5493 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 513 GATATC GAT^ATC
HaeII 2874 GGCGCT [GA]GCGC^[CT]
NciI 114 CCCGG CC^[GC]GG
NcoI 2964 CCATGG C^CATGG
NdeI 4505 CATATG CA^TATG
NheI 28 GCTAGC G^CTAGC
NruI 427 TCGCGA TCG^CGA
PaeR7I 2541 CTCGAG C^TCGAG
PflFI 570 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 5493 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 726 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 3904 CTGCAG CTGCA^G
PvuII 2121 CAGCTG CAG^CTG
SapI 2670 GAAGAGC GCTCTTC(1/4)
SnaBI 3224 TACGTA TAC^GTA
StuI 1661 AGGCCT AGG^CCT
Tth111I 570 GACGAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2541 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 41 enzymes) #
###################################
AclI 3263 AACGTT AA^CGTT
3380 AACGTT
AflIII 4561 ACATGT A^C[GA][CT]GT
4674 ACGTGT
AseI 4020 ATTAAT AT^TAAT
4625 ATTAAT
AsnI 4020 ATTAAT AT^TAAT
4625 ATTAAT
Asp700I 375 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2785 GAAGTACTTC
AvaII 2705 GGTCC G^G[AT]CC
5494 GGACC
BanII 2194 GAGCCC G[GA]GC[CT]^C
2389 GGGCTC
BbvI 1960 GCTGC GCAGC(8/12)
2123 GCTGC
BmyI 2194 GAGCCC G[AGT]GC[ACT]^C
2389 GGGCTC
BpmI 177 CTCCAG CTGGAG(16/14)
2392 CTCCAG
BsaAI 3224 TACGTA [CT]AC^GT[GA]
4673 TACGTG
BsaWI 878 TCCGGT [AT]^CCGG[AT]
1536 ACCGGA
BsmBI 21 GAGACG CGTCTC(1/5)
454 GAGACG
Bsp1286I 2194 GAGCCC G[AGT]GC[ACT]^C
2389 GGGCTC
BspHI 2917 TCATGA T^CATGA
4716 TCATGA
BsrDI 4905 CATTGC GCAATG(2/0)
2126 GCAATG
BssSI 1815 CACGAG CACGAG(-5/-1)
2637 CACGAG
BstBI 143 TTCGAA TT^CGAA
2652 TTCGAA
CfoI 32 GCGC GCG^C
2875 GCGC
EcoNI 566 CCTAGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4601 CCTGCCAAAGG
EcoRI 140 GAATTC G^AATTC
2003 GAATTC
EcoT22I 3277 ATGCAT ATGCA^T
4902 ATGCAT
HhaI 32 GCGC GCG^C
2875 GCGC
HinP1I 32 GCGC G^CGC
2875 GCGC
HincII 4557 GTTAAC GT[CT]^[GA]AC
5178 GTTAAC
HindII 4557 GTTAAC GT[CT]^[GA]AC
5178 GTTAAC
HindIII 3588 AAGCTT A^AGCTT
4078 AAGCTT
HpaI 4557 GTTAAC GTT^AAC
5178 GTTAAC
MspA1I 85 CCGCTG C[AC]G^C[GT]G
2121 CAGCTG
NsiI 3277 ATGCAT ATGCA^T
4902 ATGCAT
NspI 3275 ACATGC [GA]CATG^[CT]
4561 ACATGT
NspV 143 TTCGAA TT^CGAA
2652 TTCGAA
PshBI 4020 ATTAAT AT^TAAT
4625 ATTAAT
Psp1406I 3263 AACGTT AA^CGTT
3380 AACGTT
RcaI 2917 TCATGA T^CATGA
4716 TCATGA
ScaI 2180 AGTACT AGT^ACT
2787 AGTACT
SfcI 3135 CTATAG C^T[GA][CT]AG
3904 CTGCAG
SfuI 143 TTCGAA TT^CGAA
2652 TTCGAA
SspI 4643 AATATT AAT^ATT
5421 AATATT
TseI 1960 GCTGC G^C[AT]GC
2123 GCTGC
XmnI 375 GAACATATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2785 GAAGTACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AflII AluI AlwI ApoI BbsI
BfaI BfrI BglII BpuAI BsaBI
BsaI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsmI BspMI BsrI
BssKI Bst1107I BstF5I BstNI BstUI
BstYI BstZ17I Cac8I Cfr13I DdeI
DpnII DraI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MnlI MseI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I Tsp45I Tsp509I
TspRI TthHB8I XbaI XhoII