##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI AviII BfrI BsaBI
BseAI BsiEI BsiWI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII Bst1107I
BstZ17I ClaI CpoI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV EcoT22I FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NdeI NgoMIV
NotI NsiI PacI PaeR7I PinAI
PmeI PvuI RsrII SacII SalI
SbfI SgrAI SnaBI Sse8387I SspBI
SspI SwaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 5771 GACGTC GACGT^C
AclI 4079 AACGTT AA^CGTT
AseI 4029 ATTAAT AT^TAAT
AsnI 4029 ATTAAT AT^TAAT
AspI 4345 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 4094 GGCGCC GGCGC^C
BbrPI 817 CACGTG CAC^GTG
BglII 3800 AGATCT A^GATCT
BsaAI 817 CACGTG [CT]AC^GT[GA]
BsaI 1873 GAGACC GGTCTC(1/5)
BsmBI 4386 GAGACG CGTCTC(1/5)
BsmI 456 GAATGC GAATGC(1/-1)
BsrFI 5674 GCCGGT [GA]^CCGG[CT]
BssSI 2009 CACGAG CACGAG(-5/-1)
BstBI 2726 TTCGAA TT^CGAA
Cfr10I 5674 GCCGGT [GA]^CCGG[CT]
DraI 6584 TTTAAA TTT^AAA
EcoRI 434 GAATTC G^AATTC
KasI 4094 GGCGCC G^GCGCC
NarI 4094 GGCGCC GG^CGCC
NheI 330 GCTAGC G^CTAGC
NruI 534 TCGCGA TCG^CGA
NspI 5855 GCATGC [GA]CATG^[CT]
NspV 2726 TTCGAA TT^CGAA
PflFI 4345 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 817 CACGTG CAC^GTG
PmlI 817 CACGTG CAC^GTG
PshAI 4801 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 4029 ATTAAT AT^TAAT
Psp1406I 4079 AACGTT AA^CGTT
ScaI 663 AGTACT AGT^ACT
SexAI 2021 ACCTGGT A^CC[AT]GGT
SfoI 4094 GGCGCC GGC^GCC
SfuI 2726 TTCGAA TT^CGAA
SmaI 4403 CCCGGG CCC^GGG
SpeI 1413 ACTAGT A^CTAGT
SphI 5855 GCATGC GCATG^C
Tth111I 4345 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3453 TCTAGA T^CTAGA
XmaCI 4403 CCCGGG C^CCGGG
XmaI 4403 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccI 399 GTCTAC GT^[AC][GT]AC
5774 GTCTAC
AcsI 434 GAATTC [GA]^AATT[CT]
2269 GAATTT
ApoI 434 GAATTC [GA]^AATT[CT]
2269 GAATTT
Asp700I 430 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6614 GAAACATTTC
AvrII 18 CCTAGG C^CTAGG
340 CCTAGG
BcgI 1560 CGAGGCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
525 GCAGTGCTCTCG
BciVI 160 GGATAC GTATCC(6/5)
3908 GGATAC
BclI 821 TGATCA T^GATCA
1082 TGATCA
BlnI 18 CCTAGG C^CTAGG
340 CCTAGG
BsaWI 49 TCCGGT [AT]^CCGG[AT]
4190 ACCGGA
BspHI 56 TCATGA T^CATGA
4589 TCATGA
BsrDI 2334 CATTGC GCAATG(2/0)
3260 GCAATG
BstEII 2757 GGTGACC G^GT[ACGT]ACC
6497 GGTAACC
BstPI 2757 GGTGACC G^GT[ACGT]ACC
6497 GGTAACC
EcoO65I 2757 GGTGACC G^GT[ACGT]ACC
6497 GGTAACC
FbaI 821 TGATCA T^GATCA
1082 TGATCA
HincII 76 GTCAAC GT[CT]^[GA]AC
2732 GTCAAC
HindII 76 GTCAAC GT[CT]^[GA]AC
2732 GTCAAC
RcaI 56 TCATGA T^CATGA
4589 TCATGA
SapI 851 GCTCTTC GCTCTTC(1/4)
3432 GCTCTTC
SfiI 1465 GGCCTGTCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
3465 GGCCACTGTGGCC
XmnI 430 GAACGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6614 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII