##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AclI AflII AflIII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI BamHI
BciVI BfrI BglII BsaBI BsiWI
Bsp1407I BspDI BspHI BspLU11I BsrFI
BsrGI BssHII Bst1107I BstBI BstZ17I
Cfr10I ClaI DraI DraIII DrdI
Eam1105I Eco47III EcoNI EcoRV FseI
HincII HindII HpaI KpnI KspI
MamI MfeI MluI MunI NaeI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PvuI RcaI SacII
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 3218 GACGTC GACGT^C
AccI 2852 GTCTAC GT^[AC][GT]AC
AccIII 1830 TCCGGA T^CCGGA
ApaI 1869 GGGCCC GGGCC^C
Asp700I 532 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2950 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 1882 CACGTG CAC^GTG
BcgI 955 CGAGGCAGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 255 TGATCA T^GATCA
BlpI 2158 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2158 GCTGAGC GC^T[ACGT]AGC
BsaAI 1882 CACGTG [CT]AC^GT[GA]
BseAI 1830 TCCGGA T^CCGGA
BsiEI 2313 CGGCCG CG[GA][CT]^CG
BsmBI 554 CGTCTC CGTCTC(1/5)
BsmI 240 GCATTC GAATGC(1/-1)
BspEI 1830 TCCGGA T^CCGGA
BspMI 1464 ACCTGC ACCTGC(4/8)
BsrDI 1759 GCAATG GCAATG(2/0)
BstYI 1743 AGATCC [GA]^GATC[CT]
CelII 2158 GCTGAGC GC^T[ACGT]AGC
CpoI 2657 CGGACCG CG^G[AT]CCG
EagI 2313 CGGCCG C^GGCCG
EclXI 2313 CGGCCG C^GGCCG
Eco52I 2313 CGGCCG C^GGCCG
EcoRI 1846 GAATTC G^AATTC
EcoT22I 89 ATGCAT ATGCA^T
FbaI 255 TGATCA T^GATCA
HindIII 1063 AAGCTT A^AGCTT
MflI 1743 AGATCC [GA]^GATC[CT]
MroI 1830 TCCGGA T^CCGGA
NsiI 89 ATGCAT ATGCA^T
PflFI 2950 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1882 CACGTG CAC^GTG
PmlI 1882 CACGTG CAC^GTG
RsrII 2657 CGGACCG CG^G[AT]CCG
SphI 91 GCATGC GCATG^C
Tth111I 2950 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1743 AGATCC [GA]^GATC[CT]
XmnI 532 GAACAGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1483 GAATTT [GA]^AATT[CT]
1846 GAATTC
AocI 40 CCTCAGG CC^T[ACGT]AGG
172 CCTCAGG
ApoI 1483 GAATTT [GA]^AATT[CT]
1846 GAATTC
AviII 786 TGCGCA TGC^GCA
1788 TGCGCA
AvrII 2902 CCTAGG C^CTAGG
2933 CCTAGG
BbeI 1904 GGCGCC GGCGC^C
2152 GGCGCC
BlnI 2902 CCTAGG C^CTAGG
2933 CCTAGG
BsaI 1524 GAGACC GGTCTC(1/5)
2796 GAGACC
BssSI 2729 CACGAG CACGAG(-5/-1)
669 CTCGTG
BstAPI 467 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2341 GCAACTGCTGC
BstEII 778 GGTGACC G^GT[ACGT]ACC
935 GGTCACC
BstPI 778 GGTGACC G^GT[ACGT]ACC
935 GGTCACC
Bsu36I 40 CCTCAGG CC^T[ACGT]AGG
172 CCTCAGG
Eco81I 40 CCTCAGG CC^T[ACGT]AGG
172 CCTCAGG
EcoO65I 778 GGTGACC G^GT[ACGT]ACC
935 GGTCACC
FspI 786 TGCGCA TGC^GCA
1788 TGCGCA
KasI 1904 GGCGCC G^GCGCC
2152 GGCGCC
NarI 1904 GGCGCC GG^CGCC
2152 GGCGCC
NheI 2558 GCTAGC G^CTAGC
3053 GCTAGC
SexAI 372 ACCAGGT A^CC[AT]GGT
3837 ACCAGGT
SfoI 1904 GGCGCC GGC^GCC
2152 GGCGCC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaHI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrBI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XmaCI
XmaI