##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccII AccIII AgeI
AocI Aor51HI AscI AseI AsnI
Asp718I BamHI BbrPI BcgI BciVI
BlpI Bpu1102I BseAI BsiWI BspDI
BspEI BspHI BsrBI BssHII Bst1107I
BstBI BstUI BstZ17I Bsu36I CelII
ClaI CpoI DrdI EagI EclXI
Eco47III Eco52I Eco81I FseI HincII
HindII HpaI KpnI KspI MluI
MroI MvnI NotI NruI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshAI PshBI PvuI RcaI
RsrII SacII SalI SfiI SfuI
SgrAI SpeI SwaI XbaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 4332 CTTAAG C^TTAAG
AhdI 2000 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 940 GGGCCC GGGCC^C
AspEI 2000 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4484 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 4714 CCTAGG C^CTAGG
BfrI 4332 CTTAAG C^TTAAG
BlnI 4714 CCTAGG C^CTAGG
BsaBI 4072 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1157 GAGACC GGTCTC(1/5)
BsaWI 86 TCCGGT [AT]^CCGG[AT]
BsiEI 3618 CGACCG CG[GA][CT]^CG
BsmBI 2763 GAGACG CGTCTC(1/5)
Bsp1407I 1430 TGTACA T^GTACA
BspMI 2155 ACCTGC ACCTGC(4/8)
BsrGI 1430 TGTACA T^GTACA
BstEII 548 GGTGACC G^GT[ACGT]ACC
BstPI 548 GGTGACC G^GT[ACGT]ACC
Eam1105I 2000 GACAGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 548 GGTGACC G^GT[ACGT]ACC
EcoRV 2534 GATATC GAT^ATC
MamI 4072 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 384 GCCGGC GCC^GGC
NdeI 1886 CATATG CA^TATG
NgoMIV 384 GCCGGC G^CCGGC
NheI 5180 GCTAGC G^CTAGC
PflFI 4484 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacI 2607 GAGCTC GAGCT^C
SbfI 592 CCTGCAGG CCTGCA^GG
ScaI 4148 AGTACT AGT^ACT
SmaI 3760 CCCGGG CCC^GGG
SnaBI 3950 TACGTA TAC^GTA
SphI 2863 GCATGC GCATG^C
Sse8387I 592 CCTGCAGG CCTGCA^GG
SspBI 1430 TGTACA T^GTACA
SspI 4108 AATATT AAT^ATT
Tth111I 4484 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 3760 CCCGGG C^CCGGG
XmaI 3760 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 142 GTCTAC GT^[AC][GT]AC
2845 GTAGAC
AclI 1859 AACGTT AA^CGTT
5274 AACGTT
Alw44I 1441 GTGCAC G^TGCAC
3968 GTGCAC
ApaLI 1441 GTGCAC G^TGCAC
3968 GTGCAC
AvaI 3760 CCCGGG C^[CT]CG[GA]G
4062 CTCGGG
AviII 618 TGCGCA TGC^GCA
1101 TGCGCA
BbeI 104 GGCGCC GGCGC^C
4985 GGCGCC
BglI 1941 GCCTCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3809 GCCTATCTGGC
BsoBI 3760 CCCGGG C^[CT]CG[GA]G
4062 CTCGGG
BsrFI 384 GCCGGC [GA]^CCGG[CT]
1036 GCCGGT
BssSI 1541 CTCGTG CACGAG(-5/-1)
4699 CTCGTG
BstAPI 4201 GCATATAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
5077 GCACAAGATGC
Cfr10I 384 GCCGGC [GA]^CCGG[CT]
1036 GCCGGT
EcoRI 841 GAATTC G^AATTC
4344 GAATTC
EcoT22I 2304 ATGCAT ATGCA^T
5381 ATGCAT
FspI 618 TGCGCA TGC^GCA
1101 TGCGCA
HindIII 1048 AAGCTT A^AGCTT
2501 AAGCTT
KasI 104 GGCGCC G^GCGCC
4985 GGCGCC
MfeI 3085 CAATTG C^AATTG
5204 CAATTG
MunI 3085 CAATTG C^AATTG
5204 CAATTG
NarI 104 GGCGCC GG^CGCC
4985 GGCGCC
NcoI 1257 CCATGG C^CATGG
1945 CCATGG
NsiI 2304 ATGCAT ATGCA^T
5381 ATGCAT
Psp1406I 1859 AACGTT AA^CGTT
5274 AACGTT
SapI 48 GAAGAGC GCTCTTC(1/4)
4315 GAAGAGC
SexAI 984 ACCAGGT A^CC[AT]GGT
2369 ACCTGGT
SfoI 104 GGCGCC GGC^GCC
4985 GGCGCC
StuI 4058 AGGCCT AGG^CCT
4717 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI Asp700I
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BclI BcnI
BfaI BglII BmyI BpmI BpuAI
BsaAI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BspLU11I BsrDI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII XmnI