##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AgeI AhdI Aor51HI
AscI Asp700I Asp718I AspEI AviII
BamHI BbeI BcgI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiWI BspDI
BspEI BspLU11I BsrBI BssHII BstBI
CelII ClaI CpoI Eam1105I Eco47III
EcoRV FseI FspI HaeII KasI
KpnI KspI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PinAI PmeI PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SpeI Sse8387I
XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 4728 GACGTC GACGT^C
AccI 4845 GTATAC GT^[AC][GT]AC
AclI 2925 AACGTT AA^CGTT
AcyI 4728 GACGTC G[GA]^CG[CT]C
AflII 3459 CTTAAG C^TTAAG
Alw44I 2746 GTGCAC G^TGCAC
ApaI 1718 GGGCCC GGGCC^C
ApaLI 2746 GTGCAC G^TGCAC
AvrII 4711 CCTAGG C^CTAGG
BbvCI 3360 GCTGAGG CCTCAGC(-5/-2)
BclI 5237 TGATCA T^GATCA
BfrI 3459 CTTAAG C^TTAAG
BlnI 4711 CCTAGG C^CTAGG
BsaHI 4728 GACGTC G[GA]^CG[CT]C
BsiEI 2 CGGCCG CG[GA][CT]^CG
BsrDI 1512 CATTGC GCAATG(2/0)
BssSI 4066 CACGAG CACGAG(-5/-1)
Bst1107I 4845 GTATAC GTA^TAC
BstEII 4588 GGTCACC G^GT[ACGT]ACC
BstPI 4588 GGTCACC G^GT[ACGT]ACC
BstZ17I 4845 GTATAC GTA^TAC
EagI 2 CGGCCG C^GGCCG
EclXI 2 CGGCCG C^GGCCG
Eco52I 2 CGGCCG C^GGCCG
EcoNI 431 CCTTAGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 4588 GGTCACC G^GT[ACGT]ACC
EcoRI 5037 GAATTC G^AATTC
FbaI 5237 TGATCA T^GATCA
Hin1I 4728 GACGTC G[GA]^CG[CT]C
HpaI 727 GTTAAC GTT^AAC
NheI 4929 GCTAGC G^CTAGC
PshAI 206 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2925 AACGTT AA^CGTT
SexAI 2303 ACCTGGT A^CC[AT]GGT
SnaBI 4848 TACGTA TAC^GTA
SphI 3982 GCATGC GCATG^C
StuI 1868 AGGCCT AGG^CCT
SwaI 5572 ATTTAAAT ATTT^AAAT
XbaI 5496 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AocI 431 CCTTAGG CC^T[ACGT]AGG
740 CCTCAGG
AseI 3703 ATTAAT AT^TAAT
5432 ATTAAT
AsnI 3703 ATTAAT AT^TAAT
5432 ATTAAT
BbrPI 2313 CACGTG CAC^GTG
4543 CACGTG
BglII 1577 AGATCT A^GATCT
2111 AGATCT
BsmI 629 GAATGC GAATGC(1/-1)
4386 GAATGC
Bsp1407I 3607 TGTACA T^GTACA
5023 TGTACA
BspHI 3335 TCATGA T^CATGA
3638 TCATGA
BsrFI 522 ACCGGC [GA]^CCGG[CT]
1901 GCCGGT
BsrGI 3607 TGTACA T^GTACA
5023 TGTACA
BstXI 1356 CCAAGGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4304 CCATCATGTTGG
Bsu36I 431 CCTTAGG CC^T[ACGT]AGG
740 CCTCAGG
Cfr10I 522 ACCGGC [GA]^CCGG[CT]
1901 GCCGGT
DraI 5210 TTTAAA TTT^AAA
5573 TTTAAA
DraIII 1232 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
5073 CACAAAGTG
Eco81I 431 CCTTAGG CC^T[ACGT]AGG
740 CCTCAGG
NcoI 1409 CCATGG C^CATGG
4109 CCATGG
PmaCI 2313 CACGTG CAC^GTG
4543 CACGTG
PmlI 2313 CACGTG CAC^GTG
4543 CACGTG
PshBI 3703 ATTAAT AT^TAAT
5432 ATTAAT
RcaI 3335 TCATGA T^CATGA
3638 TCATGA
SapI 3355 GAAGAGC GCTCTTC(1/4)
3755 GCTCTTC
SmaI 1715 CCCGGG CCC^GGG
2648 CCCGGG
SspBI 3607 TGTACA T^GTACA
5023 TGTACA
XmaCI 1715 CCCGGG C^CCGGG
2648 CCCGGG
XmaI 1715 CCCGGG C^CCGGG
2648 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AspI AvaI AvaII BalI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DrdI DsaI EaeI
EarI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HincII HindII
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NsiI NspI PflFI PflMI
PleI PpuMI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII