##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccII AflIII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI BbeI BbrPI BcgI BciVI
BlpI Bpu1102I BsaBI BsiEI BsiWI
Bsp1407I BspDI BspLU11I BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI FseI HaeII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NaeI NarI NdeI
NgoMIV NotI NruI NspV PacI
PinAI PmaCI PmeI PmlI PshBI
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I SspBI SspI SwaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 4459 GACGTC GACGT^C
AccIII 3407 TCCGGA T^CCGGA
AclI 1951 AACGTT AA^CGTT
AflII 4107 CTTAAG C^TTAAG
Alw44I 989 GTGCAC G^TGCAC
ApaLI 989 GTGCAC G^TGCAC
AviII 2324 TGCGCA TGC^GCA
AvrII 4410 CCTAGG C^CTAGG
BfrI 4107 CTTAAG C^TTAAG
BglII 3399 AGATCT A^GATCT
BlnI 4410 CCTAGG C^CTAGG
BsaAI 2243 CACGTA [CT]AC^GT[GA]
BseAI 3407 TCCGGA T^CCGGA
BsmBI 4461 CGTCTC CGTCTC(1/5)
BspEI 3407 TCCGGA T^CCGGA
BspHI 1905 TCATGA T^CATGA
BsrBI 564 CCGCTC CCGCTC(-3/-3)
BsrDI 492 CATTGC GCAATG(2/0)
BsrFI 2222 ACCGGC [GA]^CCGG[CT]
BssSI 4468 CTCGTG CACGAG(-5/-1)
CfoI 2325 GCGC GCG^C
Cfr10I 2222 ACCGGC [GA]^CCGG[CT]
DrdI 1435 GACAATACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 3191 ATGCAT ATGCA^T
FspI 2324 TGCGCA TGC^GCA
HhaI 2325 GCGC GCG^C
HinP1I 2325 GCGC G^CGC
HindIII 3385 AAGCTT A^AGCTT
MroI 3407 TCCGGA T^CCGGA
NheI 1075 GCTAGC G^CTAGC
NsiI 3191 ATGCAT ATGCA^T
PaeR7I 2384 CTCGAG C^TCGAG
PshAI 3100 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1951 AACGTT AA^CGTT
PstI 3060 CTGCAG CTGCA^G
RcaI 1905 TCATGA T^CATGA
SpeI 1749 ACTAGT A^CTAGT
StuI 414 AGGCCT AGG^CCT
XhoI 2384 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 1068 GTCTAC GT^[AC][GT]AC
4311 GTAGAC
Asp700I 2268 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4781 GAACTGATTC
AspI 390 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4604 GACACAGTC
AvaI 1979 CTCGGG C^[CT]CG[GA]G
2384 CTCGAG
BamHI 20 GGATCC G^GATCC
1191 GGATCC
BclI 3177 TGATCA T^GATCA
3719 TGATCA
BcnI 7 CCGGG CC^[GC]GG
531 CCCGG
BsgI 3131 CTGCAC GTGCAG(16/14)
4681 GTGCAG
BsmI 2655 GAATGC GAATGC(1/-1)
1466 GCATTC
BsoBI 1979 CTCGGG C^[CT]CG[GA]G
2384 CTCGAG
BstEII 2131 GGTCACC G^GT[ACGT]ACC
4007 GGTCACC
BstPI 2131 GGTCACC G^GT[ACGT]ACC
4007 GGTCACC
EcoNI 163 CCTCAGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2301 CCTGCCCCAGG
EcoO65I 2131 GGTCACC G^GT[ACGT]ACC
4007 GGTCACC
EcoRV 2965 GATATC GAT^ATC
3037 GATATC
FauI 1607 CCCGC CCCGC(4/6)
1398 GCGGG
FbaI 3177 TGATCA T^GATCA
3719 TGATCA
HincII 1003 GTTGAC GT[CT]^[GA]AC
2926 GTCAAC
HindII 1003 GTTGAC GT[CT]^[GA]AC
2926 GTCAAC
MslI 888 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1486 CATGCCAGTG
NciI 7 CCGGG CC^[GC]GG
531 CCCGG
PflFI 390 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4604 GACACAGTC
SapI 789 GAAGAGC GCTCTTC(1/4)
2601 GAAGAGC
Tth111I 390 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
4604 GACACAGTC
XmnI 2268 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
4781 GAACTGATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AspHI AvaII BalI BanI BanII
BbsI BbvCI BbvI BfaI BglI
BmyI BpmI BpuAI BsaHI BsaI
BsaJI BsaWI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstXI
BstYI Bsu36I Cac8I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI Hin1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MwoI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XbaI XcmI XhoII