##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI Aor51HI AscI Asp718I
AspI AviII AvrII BalI BamHI
BbrPI BbvCI BcgI BciVI BglII
BlnI BsaAI BsaWI BseAI BsgI
BsiWI BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HpaI KpnI
KspI MaeII MfeI MluI MluNI
MroI MscI MunI NaeI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI Psp1406I PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccII 63 CGCG CG^CG
AcyI 101 GGCGCC G[GA]^CG[CT]C
AhdI 1215 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 537 GTGCAC G^TGCAC
AocI 127 CCTCAGG CC^T[ACGT]AGG
ApaI 1827 GGGCCC GGGCC^C
ApaLI 537 GTGCAC G^TGCAC
Asp700I 34 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1215 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 1827 GGGCCC G[GA]GC[CT]^C
BbeI 101 GGCGCC GGCGC^C
BclI 1100 TGATCA T^GATCA
BglI 1134 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 107 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 107 GCTCAGC GC^T[ACGT]AGC
BsaHI 101 GGCGCC G[GA]^CG[CT]C
BsiEI 29 CGATCG CG[GA][CT]^CG
BsmI 877 GCATTC GAATGC(1/-1)
BstUI 63 CGCG CG^CG
BstYI 1694 GGATCT [GA]^GATC[CT]
Bsu36I 127 CCTCAGG CC^T[ACGT]AGG
CelII 107 GCTCAGC GC^T[ACGT]AGC
CfoI 102 GCGC GCG^C
EaeI 15 CGGCCA [CT]^GGCC[GA]
Eam1105I 1215 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 127 CCTCAGG CC^T[ACGT]AGG
EcoT14I 640 CCAAGG C^C[AT][AT]GG
FbaI 1100 TGATCA T^GATCA
HaeII 101 GGCGCC [GA]GCGC^[CT]
HgaI 64 GCGTC GACGC(5/10)
HhaI 102 GCGC GCG^C
Hin1I 101 GGCGCC G[GA]^CG[CT]C
HinP1I 102 GCGC G^CGC
HindIII 1842 AAGCTT A^AGCTT
KasI 101 GGCGCC G^GCGCC
MflI 1694 GGATCT [GA]^GATC[CT]
MspA1I 2055 CAGCGG C[AC]G^C[GT]G
MvnI 63 CGCG CG^CG
NarI 101 GGCGCC GG^CGCC
PflMI 1351 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 562 GGGACCT [GA]G^G[AT]CC[CT]
PstI 900 CTGCAG CTGCA^G
PvuI 29 CGATCG CGAT^CG
SfaNI 2070 GATGC GCATC(5/9)
SfoI 101 GGCGCC GGC^GCC
SmaI 496 CCCGGG CCC^GGG
StuI 346 AGGCCT AGG^CCT
StyI 640 CCAAGG C^C[AT][AT]GG
Van91I 1351 CCAAGTACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 257 CCATATCCTTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1694 GGATCT [GA]^GATC[CT]
XmaCI 496 CCCGGG C^CCGGG
XmaI 496 CCCGGG C^CCGGG
XmnI 34 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AcsI 578 AAATTT [GA]^AATT[CT]
1622 AAATTC
AflII 2061 CTTAAG C^TTAAG
2247 CTTAAG
ApoI 578 AAATTT [GA]^AATT[CT]
1622 AAATTC
AseI 1280 ATTAAT AT^TAAT
2360 ATTAAT
AsnI 1280 ATTAAT AT^TAAT
2360 ATTAAT
AspHI 537 GTGCAC G[AT]GC[AT]^C
1788 GTGCTC
AvaI 496 CCCGGG C^[CT]CG[GA]G
1144 CTCGGG
AvaII 563 GGACC G^G[AT]CC
908 GGACC
BanI 101 GGCGCC G^G[CT][GA]CC
1675 GGTGCC
BfrI 2061 CTTAAG C^TTAAG
2247 CTTAAG
BsaBI 591 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
654 GATTACCATC
BsaI 492 GAGACC GGTCTC(1/5)
624 GGTCTC
BsiHKAI 537 GTGCAC G[AT]GC[AT]^C
1788 GTGCTC
BsmBI 65 CGTCTC CGTCTC(1/5)
1822 GAGACG
BsmFI 222 GGGAC GGGAC(10/14)
562 GGGAC
BsoBI 496 CCCGGG C^[CT]CG[GA]G
1144 CTCGGG
Bsp1407I 1043 TGTACA T^GTACA
1937 TGTACA
BspMI 1415 ACCTGC ACCTGC(4/8)
902 GCAGGT
BsrBI 105 CCGCTC CCGCTC(-3/-3)
1679 CCGCTC
BsrGI 1043 TGTACA T^GTACA
1937 TGTACA
DraI 2204 TTTAAA TTT^AAA
2270 TTTAAA
DraII 81 AGGCCCC [GA]G^G[ACGT]CC[CT]
562 GGGACCT
EarI 486 GAAGAG CTCTTC(1/4)
1476 GAAGAG
EcoO109I 81 AGGCCCC [GA]G^G[ACGT]CC[CT]
562 GGGACCT
FauI 4 GCGGG CCCGC(4/6)
1275 GCGGG
Ksp632I 486 GAAGAG CTCTTC(1/4)
1476 GAAGAG
MamI 591 GATGAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
654 GATTACCATC
NdeI 1710 CATATG CA^TATG
2393 CATATG
NspI 669 ACATGC [GA]CATG^[CT]
1925 GCATGT
PshAI 618 GACTTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1649 GACAGGTGTC
PshBI 1280 ATTAAT AT^TAAT
2360 ATTAAT
SfcI 663 CTACAG C^T[GA][CT]AG
900 CTGCAG
SspBI 1043 TGTACA T^GTACA
1937 TGTACA
SspI 196 AATATT AAT^ATT
413 AATATT
TaqI 32 TCGA T^CGA
1015 TCGA
TthHB8I 32 TCGA T^CGA
1015 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 64 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScaI ScrFI SmlI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI