##################################
# NO CUT ENZYMES ( 62 enzymes) #
##################################
AatII AccII AccIII AclI AflII
Alw44I ApaLI AscI AspI BbrPI
BcgI BfrI BseAI BsiWI BsmBI
Bsp1407I BspDI BspEI BsrBI BsrGI
BssHII Bst1107I BstUI BstZ17I ClaI
CpoI DrdI FseI HpaI KspI
MfeI MluI MroI MunI MvnI
NaeI NgoMIV NotI NruI PacI
PaeR7I PflFI PmaCI PmeI PmlI
PshAI Psp1406I PvuI RsrII SacII
SbfI SgrAI SmaI SphI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 623 GGTACC G^GTACC
AcyI 2698 GGCGCC G[GA]^CG[CT]C
AflIII 2507 ACATGT A^C[GA][CT]GT
AhdI 3376 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 4869 CCTGAGG CC^T[ACGT]AGG
AseI 2242 ATTAAT AT^TAAT
AsnI 2242 ATTAAT AT^TAAT
Asp718I 623 GGTACC G^GTACC
AspEI 3376 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2692 CTCGGG C^[CT]CG[GA]G
AviII 2357 TGCGCA TGC^GCA
BamHI 3730 GGATCC G^GATCC
BbeI 2698 GGCGCC GGCGC^C
BclI 3632 TGATCA T^GATCA
BsaAI 5285 TACGTA [CT]AC^GT[GA]
BsaBI 5439 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2698 GGCGCC G[GA]^CG[CT]C
BsgI 2101 GTGCAG GTGCAG(16/14)
BsoBI 2692 CTCGGG C^[CT]CG[GA]G
BspLU11I 2507 ACATGT A^CATGT
BspMI 164 ACCTGC ACCTGC(4/8)
BssSI 2921 CACGAG CACGAG(-5/-1)
BstBI 3175 TTCGAA TT^CGAA
BstEII 4900 GGTAACC G^GT[ACGT]ACC
BstPI 4900 GGTAACC G^GT[ACGT]ACC
Bsu36I 4869 CCTGAGG CC^T[ACGT]AGG
DraI 5115 TTTAAA TTT^AAA
EagI 1548 CGGCCG C^GGCCG
Eam1105I 3376 GACAACTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 1548 CGGCCG C^GGCCG
Eco52I 1548 CGGCCG C^GGCCG
Eco81I 4869 CCTGAGG CC^T[ACGT]AGG
EcoO65I 4900 GGTAACC G^GT[ACGT]ACC
EcoRI 5154 GAATTC G^AATTC
EcoT22I 5148 ATGCAT ATGCA^T
FbaI 3632 TGATCA T^GATCA
FspI 2357 TGCGCA TGC^GCA
HgaI 2094 GCGTC GACGC(5/10)
Hin1I 2698 GGCGCC G[GA]^CG[CT]C
KasI 2698 GGCGCC G^GCGCC
KpnI 623 GGTACC GGTAC^C
MamI 5439 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 2698 GGCGCC GG^CGCC
NheI 2220 GCTAGC G^CTAGC
NsiI 5148 ATGCAT ATGCA^T
NspV 3175 TTCGAA TT^CGAA
PshBI 2242 ATTAAT AT^TAAT
ScaI 2138 AGTACT AGT^ACT
SfiI 3991 GGCCCCTTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2698 GGCGCC GGC^GCC
SfuI 3175 TTCGAA TT^CGAA
SnaBI 5285 TACGTA TAC^GTA
SpeI 4558 ACTAGT A^CTAGT
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AgeI 620 ACCGGT A^CCGGT
1127 ACCGGT
Aor51HI 2036 AGCGCT AGC^GCT
3194 AGCGCT
ApaI 3294 GGGCCC GGGCC^C
3990 GGGCCC
Asp700I 3357 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5154 GAATTCATTC
AvrII 4116 CCTAGG C^CTAGG
5580 CCTAGG
BalI 1394 TGGCCA TGG^CCA
2633 TGGCCA
BbvCI 265 GCTGAGG CCTCAGC(-5/-2)
2983 GCTGAGG
BciVI 4045 GTATCC GTATCC(6/5)
4951 GTATCC
BglII 3248 AGATCT A^GATCT
5384 AGATCT
BlnI 4116 CCTAGG C^CTAGG
5580 CCTAGG
BsaWI 620 ACCGGT [AT]^CCGG[AT]
1127 ACCGGT
BsiEI 1467 CGACCG CG[GA][CT]^CG
1548 CGGCCG
BspHI 2664 TCATGA T^CATGA
4365 TCATGA
BsrDI 1881 CATTGC GCAATG(2/0)
1841 GCAATG
BsrFI 620 ACCGGT [GA]^CCGG[CT]
1127 ACCGGT
BstAPI 1266 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3039 GCAGAGTGTGC
Cfr10I 620 ACCGGT [GA]^CCGG[CT]
1127 ACCGGT
DraIII 4011 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4702 CACTAGGTG
Eco47III 2036 AGCGCT AGC^GCT
3194 AGCGCT
EcoRV 2248 GATATC GAT^ATC
5746 GATATC
HincII 50 GTCGAC GT[CT]^[GA]AC
3531 GTCGAC
HindII 50 GTCGAC GT[CT]^[GA]AC
3531 GTCGAC
HindIII 679 AAGCTT A^AGCTT
2752 AAGCTT
MluNI 1394 TGGCCA TGG^CCA
2633 TGGCCA
MscI 1394 TGGCCA TGG^CCA
2633 TGGCCA
MspA1I 218 CAGCTG C[AC]G^C[GT]G
664 CAGCTG
PinAI 620 ACCGGT A^CCGGT
1127 ACCGGT
PpuMI 185 GGGACCT [GA]G^G[AT]CC[CT]
4182 AGGACCT
PvuII 218 CAGCTG CAG^CTG
664 CAGCTG
RcaI 2664 TCATGA T^CATGA
4365 TCATGA
SalI 50 GTCGAC G^TCGAC
3531 GTCGAC
SapI 1348 GAAGAGC GCTCTTC(1/4)
3310 GAAGAGC
StuI 2792 AGGCCT AGG^CCT
4679 AGGCCT
XmnI 3357 GAAACGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
5154 GAATTCATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI BstYI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspI
MvaI MwoI NciI NcoI NdeI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmlI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI XhoII