##################################
# NO CUT ENZYMES ( 64 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI AscI Asp718I
AspEI AspI AvrII BbeI BclI
BfrI BglII BlnI BsaWI BseAI
BsiWI BspEI BspHI BssHII Bst1107I
BstZ17I CpoI Eam1105I EcoRV EcoT22I
FbaI FseI HindIII KasI KpnI
MfeI MluI MroI MunI NarI
NdeI NheI NotI NruI NsiI
PacI PflFI PinAI PmeI PshAI
Psp1406I PvuI RcaI RsrII SalI
SbfI SexAI SfiI SfoI SgrAI
SphI Sse8387I Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
Aor51HI 5 AGCGCT AGC^GCT
AseI 4646 ATTAAT AT^TAAT
AsnI 4646 ATTAAT AT^TAAT
Asp700I 211 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 3186 TGCGCA TGC^GCA
BalI 2228 TGGCCA TGG^CCA
BamHI 4018 GGATCC G^GATCC
BanI 25 GGCACC G^G[CT][GA]CC
BbrPI 1334 CACGTG CAC^GTG
BciVI 772 GTATCC GTATCC(6/5)
BsaBI 571 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 700 GAGACG CGTCTC(1/5)
BsmI 2329 GAATGC GAATGC(1/-1)
BspDI 589 ATCGAT AT^CGAT
BssSI 2403 CACGAG CACGAG(-5/-1)
BstBI 661 TTCGAA TT^CGAA
BstXI 2267 CCAGGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 589 ATCGAT AT^CGAT
DraIII 2179 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4614 GACTTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5 AGCGCT AGC^GCT
EcoRI 2095 GAATTC G^AATTC
FspI 3186 TGCGCA TGC^GCA
HpaI 265 GTTAAC GTT^AAC
KspI 29 CCGCGG CCGC^GG
MamI 571 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2228 TGGCCA TGG^CCA
MscI 2228 TGGCCA TGG^CCA
NspV 661 TTCGAA TT^CGAA
PaeR7I 2144 CTCGAG C^TCGAG
PmaCI 1334 CACGTG CAC^GTG
PmlI 1334 CACGTG CAC^GTG
PshBI 4646 ATTAAT AT^TAAT
PvuII 2856 CAGCTG CAG^CTG
SacII 29 CCGCGG CCGC^GG
ScaI 4945 AGTACT AGT^ACT
SfuI 661 TTCGAA TT^CGAA
SmaI 2985 CCCGGG CCC^GGG
SnaBI 3967 TACGTA TAC^GTA
SpeI 4923 ACTAGT A^CTAGT
SwaI 4640 ATTTAAAT ATTT^AAAT
XcmI 2213 CCAGCTCACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2144 CTCGAG C^TCGAG
XmaCI 2985 CCCGGG C^CCGGG
XmaI 2985 CCCGGG C^CCGGG
XmnI 211 GAAGAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Alw44I 2400 GTGCAC G^TGCAC
3242 GTGCAC
ApaLI 2400 GTGCAC G^TGCAC
3242 GTGCAC
BcgI 1344 CGAGCCAGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3509 GCACACGGTTCG
BsaAI 1334 CACGTG [CT]AC^GT[GA]
3967 TACGTA
BsaI 2565 GAGACC GGTCTC(1/5)
3758 GGTCTC
BsiEI 41 CGGCCG CG[GA][CT]^CG
86 CGGCCG
Bsp1407I 2323 TGTACA T^GTACA
4716 TGTACA
BspLU11I 1499 ACATGT A^CATGT
2847 ACATGT
BsrBI 143 GAGCGG CCGCTC(-3/-3)
3425 GAGCGG
BsrGI 2323 TGTACA T^GTACA
4716 TGTACA
EagI 41 CGGCCG C^GGCCG
86 CGGCCG
EclXI 41 CGGCCG C^GGCCG
86 CGGCCG
Eco52I 41 CGGCCG C^GGCCG
86 CGGCCG
EcoNI 456 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1660 CCTCCCACAGG
HaeII 5 AGCGCT [GA]GCGC^[CT]
977 AGCGCC
NaeI 84 GCCGGC GCC^GGC
1629 GCCGGC
NcoI 3152 CCATGG C^CATGG
4698 CCATGG
NgoMIV 84 GCCGGC G^CCGGC
1629 GCCGGC
SspBI 2323 TGTACA T^GTACA
4716 TGTACA
SspI 4121 AATATT AAT^ATT
4667 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApaI ApoI AspHI AvaI AvaII
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrDI
BsrFI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII