##################################
# NO CUT ENZYMES ( 54 enzymes) #
##################################
AatII AccIII AgeI AhdI Alw44I
ApaLI AscI AspEI AspI BamHI
BseAI BsiWI BspDI BspEI BssHII
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraIII DrdI Eam1105I EcoT22I
FseI MfeI MluI MroI MunI
NaeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PvuI RsrII SbfI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AclI 2132 AACGTT AA^CGTT
AcyI 50 GGCGCC G[GA]^CG[CT]C
AflII 2254 CTTAAG C^TTAAG
AflIII 3885 ACATGT A^C[GA][CT]GT
AocI 1046 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1868 AGCGCT AGC^GCT
Asp700I 310 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 762 TGCGCA TGC^GCA
BbeI 50 GGCGCC GGCGC^C
BcgI 2992 CGATCCTTCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3798 GGATAC GTATCC(6/5)
BfrI 2254 CTTAAG C^TTAAG
BlpI 73 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC
BsaBI 3053 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 50 GGCGCC G[GA]^CG[CT]C
BsaWI 607 TCCGGT [AT]^CCGG[AT]
BsmBI 1369 GAGACG CGTCTC(1/5)
BspLU11I 3885 ACATGT A^CATGT
BsrDI 4414 CATTGC GCAATG(2/0)
BsrFI 140 GCCGGT [GA]^CCGG[CT]
BssSI 2124 CACGAG CACGAG(-5/-1)
Bsu36I 1046 CCTGAGG CC^T[ACGT]AGG
CelII 73 GCTGAGC GC^T[ACGT]AGC
Cfr10I 140 GCCGGT [GA]^CCGG[CT]
DraI 5035 TTTAAA TTT^AAA
Eco47III 1868 AGCGCT AGC^GCT
Eco81I 1046 CCTGAGG CC^T[ACGT]AGG
EcoRV 2063 GATATC GAT^ATC
FspI 762 TGCGCA TGC^GCA
HgaI 1371 GACGC GACGC(5/10)
Hin1I 50 GGCGCC G[GA]^CG[CT]C
HindIII 4175 AAGCTT A^AGCTT
HpaI 4328 GTTAAC GTT^AAC
KasI 50 GGCGCC G^GCGCC
KspI 18 CCGCGG CCGC^GG
MamI 3053 GATTCATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 50 GGCGCC GG^CGCC
NdeI 4453 CATATG CA^TATG
NheI 3136 GCTAGC G^CTAGC
PshAI 1250 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2132 AACGTT AA^CGTT
SacII 18 CCGCGG CCGC^GG
SalI 1061 GTCGAC G^TCGAC
ScaI 312 AGTACT AGT^ACT
SfoI 50 GGCGCC GGC^GCC
SphI 3029 GCATGC GCATG^C
StuI 1279 AGGCCT AGG^CCT
XbaI 3849 TCTAGA T^CTAGA
XcmI 1981 CCACTGCAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 310 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 1640 GGTACC G^GTACC
3622 GGTACC
AccI 962 GTCTAC GT^[AC][GT]AC
1061 GTCGAC
AccII 19 CGCG CG^CG
58 CGCG
AlwNI 1253 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
3323 CAGGCACTG
ApaI 2170 GGGCCC GGGCC^C
2882 GGGCCC
Asp718I 1640 GGTACC G^GTACC
3622 GGTACC
BbrPI 1433 CACGTG CAC^GTG
4029 CACGTG
BclI 2952 TGATCA T^GATCA
3597 TGATCA
BsaAI 1433 CACGTG [CT]AC^GT[GA]
4029 CACGTG
BsiEI 68 CGGCCG CG[GA][CT]^CG
138 CGGCCG
Bsp1407I 1068 TGTACA T^GTACA
2620 TGTACA
BsrBI 104 CCGCTC CCGCTC(-3/-3)
1139 GAGCGG
BsrGI 1068 TGTACA T^GTACA
2620 TGTACA
BstEII 143 GGTTACC G^GT[ACGT]ACC
3668 GGTTACC
BstPI 143 GGTTACC G^GT[ACGT]ACC
3668 GGTTACC
BstUI 19 CGCG CG^CG
58 CGCG
BstXI 1090 CCACTTTATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4835 CCAGCTGCTTGG
EagI 68 CGGCCG C^GGCCG
138 CGGCCG
EclXI 68 CGGCCG C^GGCCG
138 CGGCCG
Eco52I 68 CGGCCG C^GGCCG
138 CGGCCG
EcoO65I 143 GGTTACC G^GT[ACGT]ACC
3668 GGTTACC
FbaI 2952 TGATCA T^GATCA
3597 TGATCA
HincII 1061 GTCGAC GT[CT]^[GA]AC
4328 GTTAAC
HindII 1061 GTCGAC GT[CT]^[GA]AC
4328 GTTAAC
KpnI 1640 GGTACC GGTAC^C
3622 GGTACC
MvnI 19 CGCG CG^CG
58 CGCG
PmaCI 1433 CACGTG CAC^GTG
4029 CACGTG
PmlI 1433 CACGTG CAC^GTG
4029 CACGTG
SmaI 36 CCCGGG CCC^GGG
109 CCCGGG
SspBI 1068 TGTACA T^GTACA
2620 TGTACA
SspI 4490 AATATT AAT^ATT
5005 AATATT
TaqI 1062 TCGA T^CGA
4617 TCGA
TthHB8I 1062 TCGA T^CGA
4617 TCGA
XmaCI 36 CCCGGG C^CCGGG
109 CCCGGG
XmaI 36 CCCGGG C^CCGGG
109 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaI
AvaII AvrII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BglII BlnI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsmI BsoBI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PshBI PstI PvuII RcaI
RsaI SacI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XhoII