##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII Acc65I AccIII AclI AflIII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp718I AspEI AviII
AvrII BamHI BbvCI BciVI BlnI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspLU11I BsrDI BstEII BstPI
ClaI Eam1105I EcoO65I FseI FspI
KpnI KspI MluI MroI NheI
NotI NruI PacI PinAI PshAI
PshBI Psp1406I PvuI SacII SalI
SexAI SgrAI SnaBI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccII 275 CGCG CG^CG
AflII 3072 CTTAAG C^TTAAG
Aor51HI 3282 AGCGCT AGC^GCT
BbeI 1137 GGCGCC GGCGC^C
BbrPI 1320 CACGTG CAC^GTG
BcgI 1488 GCAGAAGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3072 CTTAAG C^TTAAG
BglII 725 AGATCT A^GATCT
BlpI 2516 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2516 GCTCAGC GC^T[ACGT]AGC
BsaAI 1320 CACGTG [CT]AC^GT[GA]
BsiEI 1771 CGGCCG CG[GA][CT]^CG
Bsp1407I 3419 TGTACA T^GTACA
BspHI 2299 TCATGA T^CATGA
BsrGI 3419 TGTACA T^GTACA
BssHII 274 GCGCGC G^CGCGC
Bst1107I 4581 GTATAC GTA^TAC
BstAPI 2328 GCAAATGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 4373 TTCGAA TT^CGAA
BstUI 275 CGCG CG^CG
BstZ17I 4581 GTATAC GTA^TAC
CelII 2516 GCTCAGC GC^T[ACGT]AGC
CpoI 1022 CGGTCCG CG^G[AT]CCG
DraIII 4030 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2768 GACTAGCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1771 CGGCCG C^GGCCG
EclXI 1771 CGGCCG C^GGCCG
Eco47III 3282 AGCGCT AGC^GCT
Eco52I 1771 CGGCCG C^GGCCG
EcoRI 2278 GAATTC G^AATTC
EcoRV 3480 GATATC GAT^ATC
HincII 4267 GTTAAC GT[CT]^[GA]AC
HindII 4267 GTTAAC GT[CT]^[GA]AC
HindIII 3769 AAGCTT A^AGCTT
HpaI 4267 GTTAAC GTT^AAC
KasI 1137 GGCGCC G^GCGCC
MfeI 952 CAATTG C^AATTG
MunI 952 CAATTG C^AATTG
MvnI 275 CGCG CG^CG
NaeI 3158 GCCGGC GCC^GGC
NarI 1137 GGCGCC GG^CGCC
NcoI 518 CCATGG C^CATGG
NdeI 3723 CATATG CA^TATG
NgoMIV 3158 GCCGGC G^CCGGC
NspV 4373 TTCGAA TT^CGAA
PmaCI 1320 CACGTG CAC^GTG
PmeI 3608 GTTTAAAC GTTT^AAAC
PmlI 1320 CACGTG CAC^GTG
RcaI 2299 TCATGA T^CATGA
RsrII 1022 CGGTCCG CG^G[AT]CCG
SbfI 2894 CCTGCAGG CCTGCA^GG
ScaI 4115 AGTACT AGT^ACT
SfoI 1137 GGCGCC GGC^GCC
SfuI 4373 TTCGAA TT^CGAA
SpeI 972 ACTAGT A^CTAGT
SphI 2337 GCATGC GCATG^C
Sse8387I 2894 CCTGCAGG CCTGCA^GG
SspBI 3419 TGTACA T^GTACA
SspI 3916 AATATT AAT^ATT
StuI 229 AGGCCT AGG^CCT
SwaI 3259 ATTTAAAT ATTT^AAAT
XbaI 915 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 51 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2205 CAGGCGCTG
AocI 1452 CCTCAGG CC^T[ACGT]AGG
1638 CCTGAGG
ApaI 790 GGGCCC GGGCC^C
3196 GGGCCC
AspI 171 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACCCGGTC
BsaBI 474 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
720 GATGAAGATC
BsaI 1465 GAGACC GGTCTC(1/5)
1474 GAGACC
Bsu36I 1452 CCTCAGG CC^T[ACGT]AGG
1638 CCTGAGG
Eco81I 1452 CCTCAGG CC^T[ACGT]AGG
1638 CCTGAGG
MamI 474 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
720 GATGAAGATC
PaeR7I 279 CTCGAG C^TCGAG
2508 CTCGAG
PflFI 171 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACCCGGTC
PflMI 518 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
794 CCACCAGCTGG
PpuMI 1759 AGGACCT [GA]G^G[AT]CC[CT]
3120 GGGTCCC
PvuII 798 CAGCTG CAG^CTG
3313 CAGCTG
SacI 252 GAGCTC GAGCT^C
2247 GAGCTC
SfiI 2048 GGCCTTCCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2868 GGCCGTGGGGGCC
SmaI 61 CCCGGG CCC^GGG
1142 CCCGGG
Tth111I 171 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1018 GACCCGGTC
Van91I 518 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
794 CCACCAGCTGG
XcmI 1591 CCAGGAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2107 CCACTCACCTCATGG
XhoI 279 CTCGAG C^TCGAG
2508 CTCGAG
XmaCI 61 CCCGGG C^CCGGG
1142 CCCGGG
XmaI 61 CCCGGG C^CCGGG
1142 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BclI BcnI BfaI
BglI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BssSI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I EcoT22I FauI FbaI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PstI RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI