##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AccI AclI AgeI AscI AseI
AsnI AvrII BbrPI BclI BglII
BlnI BsaAI BsaBI BsiEI BsiWI
Bsp1407I BspDI BsrGI BssHII BssSI
Bst1107I BstZ17I ClaI CpoI EagI
EclXI Eco52I EcoT22I FbaI HpaI
MamI MfeI MluI MunI NheI
NotI NruI NsiI PacI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SgrAI SmaI
SnaBI SspBI SspI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AatII 5477 GACGTC GACGT^C
AccIII 1516 TCCGGA T^CCGGA
AflII 4976 CTTAAG C^TTAAG
Aor51HI 5547 AGCGCT AGC^GCT
ApaI 4971 GGGCCC GGGCC^C
AviII 2514 TGCGCA TGC^GCA
BbeI 5234 GGCGCC GGCGC^C
BfrI 4976 CTTAAG C^TTAAG
BseAI 1516 TCCGGA T^CCGGA
BspEI 1516 TCCGGA T^CCGGA
BspLU11I 5046 ACATGT A^CATGT
BstBI 3375 TTCGAA TT^CGAA
BstEII 2386 GGTGACC G^GT[ACGT]ACC
BstPI 2386 GGTGACC G^GT[ACGT]ACC
DraI 5753 TTTAAA TTT^AAA
DrdI 3163 GACTGTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 5547 AGCGCT AGC^GCT
EcoO65I 2386 GGTGACC G^GT[ACGT]ACC
EcoRI 3570 GAATTC G^AATTC
EcoRV 1377 GATATC GAT^ATC
FseI 3481 GGCCGGCC GGCCGG^CC
FspI 2514 TGCGCA TGC^GCA
KasI 5234 GGCGCC G^GCGCC
KspI 4851 CCGCGG CCGC^GG
MroI 1516 TCCGGA T^CCGGA
NarI 5234 GGCGCC GG^CGCC
NdeI 1434 CATATG CA^TATG
NspV 3375 TTCGAA TT^CGAA
PaeR7I 5709 CTCGAG C^TCGAG
SacII 4851 CCGCGG CCGC^GG
SapI 2248 GAAGAGC GCTCTTC(1/4)
ScaI 1763 AGTACT AGT^ACT
SfoI 5234 GGCGCC GGC^GCC
SfuI 3375 TTCGAA TT^CGAA
SpeI 5652 ACTAGT A^CTAGT
SphI 600 GCATGC GCATG^C
StuI 1266 AGGCCT AGG^CCT
SwaI 5752 ATTTAAAT ATTT^AAAT
XbaI 3244 TCTAGA T^CTAGA
XhoI 5709 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 614 GGTACC G^GTACC
1477 GGTACC
AhdI 882 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2958 GACAGGGTGTC
Alw44I 1061 GTGCAC G^TGCAC
2756 GTGCAC
ApaLI 1061 GTGCAC G^TGCAC
2756 GTGCAC
Asp718I 614 GGTACC G^GTACC
1477 GGTACC
AspEI 882 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2958 GACAGGGTGTC
BcgI 1112 CGACTCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4581 CGAATGTGCTGC
BciVI 620 GTATCC GTATCC(6/5)
3225 GTATCC
BsaWI 1516 TCCGGA [AT]^CCGG[AT]
5184 ACCGGA
BspHI 1381 TCATGA T^CATGA
2319 TCATGA
BsrBI 5372 GAGCGG CCGCTC(-3/-3)
5495 GAGCGG
Eam1105I 882 GACTGGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2958 GACAGGGTGTC
KpnI 614 GGTACC GGTAC^C
1477 GGTACC
PshAI 2441 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
5071 GACCAAGGTC
PvuII 4634 CAGCTG CAG^CTG
5357 CAGCTG
RcaI 1381 TCATGA T^CATGA
2319 TCATGA
SbfI 1904 CCTGCAGG CCTGCA^GG
4927 CCTGCAGG
SexAI 2652 ACCTGGT A^CC[AT]GGT
4206 ACCTGGT
SfiI 3643 GGCCCTGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
5692 GGCCAGCCCGGCC
Sse8387I 1904 CCTGCAGG CCTGCA^GG
4927 CCTGCAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI AlwNI AocI
ApoI Asp700I AspHI AspI AvaI
AvaII BalI BamHI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlpI BmyI BpmI Bpu1102I
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PflFI PflMI PleI PpuMI
PstI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I Van91I
XcmI XhoII XmnI