##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI Alw44I AlwNI AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspI AvaII AviII BamHI
BbrPI BcgI BfrI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrFI BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
FseI FspI HincII HindII HpaI
KpnI MaeII MamI MluI MroI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI RsrII SalI
SapI SbfI ScaI SexAI SfuI
SgrAI SnaBI SpeI Sse8387I SwaI
Tth111I XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 3047 GTCTAC GT^[AC][GT]AC
AcyI 19 GGCGCC G[GA]^CG[CT]C
AhdI 654 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 654 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 2630 CCCGGG C^[CT]CG[GA]G
AvrII 2984 CCTAGG C^CTAGG
BbeI 19 GGCGCC GGCGC^C
BbvCI 2722 GCTGAGG CCTCAGC(-5/-2)
BciVI 3153 GTATCC GTATCC(6/5)
BglII 449 AGATCT A^GATCT
BlnI 2984 CCTAGG C^CTAGG
BsaHI 19 GGCGCC G[GA]^CG[CT]C
BsaI 942 GGTCTC GGTCTC(1/5)
BsaWI 543 TCCGGT [AT]^CCGG[AT]
BsmBI 2651 CGTCTC CGTCTC(1/5)
BsmFI 1428 GGGAC GGGAC(10/14)
BsoBI 2630 CCCGGG C^[CT]CG[GA]G
BspHI 680 TCATGA T^CATGA
BssHII 138 GCGCGC G^CGCGC
DrdI 1488 GACTGAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 654 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1504 GAAGAG CTCTTC(1/4)
EcoRV 2102 GATATC GAT^ATC
EcoT22I 393 ATGCAT ATGCA^T
FauI 73 GCGGG CCCGC(4/6)
HaeII 19 GGCGCC [GA]GCGC^[CT]
Hin1I 19 GGCGCC G[GA]^CG[CT]C
KasI 19 GGCGCC G^GCGCC
Ksp632I 1504 GAAGAG CTCTTC(1/4)
MfeI 3136 CAATTG C^AATTG
MunI 3136 CAATTG C^AATTG
NarI 19 GGCGCC GG^CGCC
NsiI 393 ATGCAT ATGCA^T
PflMI 2426 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 2761 CTGCAG CTGCA^G
RcaI 680 TCATGA T^CATGA
SacI 2608 GAGCTC GAGCT^C
SfiI 2012 GGCCACCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 19 GGCGCC GGC^GCC
SmaI 2630 CCCGGG CCC^GGG
SphI 2693 GCATGC GCATG^C
SspI 2946 AATATT AAT^ATT
StuI 2585 AGGCCT AGG^CCT
Van91I 2426 CCATGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1658 TCTAGA T^CTAGA
XmaCI 2630 CCCGGG C^CCGGG
XmaI 2630 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AseI 2311 ATTAAT AT^TAAT
2991 ATTAAT
AsnI 2311 ATTAAT AT^TAAT
2991 ATTAAT
BalI 1937 TGGCCA TGG^CCA
2372 TGGCCA
BanI 19 GGCGCC G^G[CT][GA]CC
2884 GGCACC
BanII 27 GAGCCC G[GA]GC[CT]^C
2608 GAGCTC
BclI 648 TGATCA T^GATCA
1401 TGATCA
BglI 142 GCCCGAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2013 GCCACCGCGGC
BsmI 2247 GAATGC GAATGC(1/-1)
1066 GCATTC
BstYI 449 AGATCT [GA]^GATC[CT]
1167 AGATCC
FbaI 648 TGATCA T^GATCA
1401 TGATCA
HindIII 204 AAGCTT A^AGCTT
1678 AAGCTT
KspI 71 CCGCGG CCGC^GG
2017 CCGCGG
MflI 449 AGATCT [GA]^GATC[CT]
1167 AGATCC
MluNI 1937 TGGCCA TGG^CCA
2372 TGGCCA
MscI 1937 TGGCCA TGG^CCA
2372 TGGCCA
NcoI 111 CCATGG C^CATGG
1368 CCATGG
NlaIV 19 GGCGCC GG[ACGT]^[ACGT]CC
2884 GGCACC
NspI 1248 ACATGC [GA]CATG^[CT]
2693 GCATGC
PshBI 2311 ATTAAT AT^TAAT
2991 ATTAAT
SacII 71 CCGCGG CCGC^GG
2017 CCGCGG
XhoII 449 AGATCT [GA]^GATC[CT]
1167 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI Bsp1286I Bsp1407I BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraI DsaI EaeI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII PleI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I