##################################
# NO CUT ENZYMES ( 47 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Aor51HI AscI Asp718I AviII BcgI
BfrI BsiWI CpoI DraIII EagI
EclXI Eco47III Eco52I EcoRV FseI
FspI HpaI KpnI KspI MfeI
MluI MunI NheI NotI NruI
PacI PaeR7I PflMI PinAI PmeI
PshAI Psp1406I RsrII SacII SapI
ScaI SfiI SgrAI SnaBI Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccIII 1289 TCCGGA T^CCGGA
AhdI 4413 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 2732 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 3767 ATTAAT AT^TAAT
AsnI 3767 ATTAAT AT^TAAT
AspEI 4413 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbeI 9 GGCGCC GGCGC^C
BclI 310 TGATCA T^GATCA
BseAI 1289 TCCGGA T^CCGGA
BsiEI 4271 CGATCG CG[GA][CT]^CG
BsmBI 458 GAGACG CGTCTC(1/5)
Bsp1407I 4928 TGTACA T^GTACA
BspEI 1289 TCCGGA T^CCGGA
BspHI 1650 TCATGA T^CATGA
BsrBI 13 CCGCTC CCGCTC(-3/-3)
BsrGI 4928 TGTACA T^GTACA
BssHII 1840 GCGCGC G^CGCGC
Bst1107I 5072 GTATAC GTA^TAC
BstBI 4032 TTCGAA TT^CGAA
BstEII 735 GGTCACC G^GT[ACGT]ACC
BstPI 735 GGTCACC G^GT[ACGT]ACC
BstZ17I 5072 GTATAC GTA^TAC
DrdI 122 GACATCATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4413 GACTCCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 735 GGTCACC G^GT[ACGT]ACC
EcoRI 5138 GAATTC G^AATTC
EcoT22I 1470 ATGCAT ATGCA^T
FbaI 310 TGATCA T^GATCA
HindIII 1936 AAGCTT A^AGCTT
KasI 9 GGCGCC G^GCGCC
MroI 1289 TCCGGA T^CCGGA
NarI 9 GGCGCC GG^CGCC
NcoI 600 CCATGG C^CATGG
NdeI 257 CATATG CA^TATG
NsiI 1470 ATGCAT ATGCA^T
NspV 4032 TTCGAA TT^CGAA
PshBI 3767 ATTAAT AT^TAAT
PvuI 4271 CGATCG CGAT^CG
RcaI 1650 TCATGA T^CATGA
SalI 2564 GTCGAC G^TCGAC
SbfI 4689 CCTGCAGG CCTGCA^GG
SexAI 5099 ACCTGGT A^CC[AT]GGT
SfoI 9 GGCGCC GGC^GCC
SfuI 4032 TTCGAA TT^CGAA
SmaI 2858 CCCGGG CCC^GGG
SphI 4777 GCATGC GCATG^C
Sse8387I 4689 CCTGCAGG CCTGCA^GG
SspBI 4928 TGTACA T^GTACA
SwaI 4088 ATTTAAAT ATTT^AAAT
XmaCI 2858 CCCGGG C^CCGGG
XmaI 2858 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 567 ACATGT A^C[GA][CT]GT
5051 ACATGT
Alw44I 2774 GTGCAC G^TGCAC
3103 GTGCAC
ApaLI 2774 GTGCAC G^TGCAC
3103 GTGCAC
Asp700I 2592 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2835 GAAGCATTTC
AvrII 3035 CCTAGG C^CTAGG
3071 CCTAGG
BamHI 1243 GGATCC G^GATCC
4722 GGATCC
BanI 9 GGCGCC G^G[CT][GA]CC
2015 GGCACC
BbrPI 1999 CACGTG CAC^GTG
3011 CACGTG
BciVI 2620 GGATAC GTATCC(6/5)
4641 GTATCC
BglI 2269 GCCCCAGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2975 GCCTTTCCGGC
BglII 4260 AGATCT A^GATCT
4804 AGATCT
BlnI 3035 CCTAGG C^CTAGG
3071 CCTAGG
BlpI 1789 GCTCAGC GC^T[ACGT]AGC
2144 GCTAAGC
Bpu1102I 1789 GCTCAGC GC^T[ACGT]AGC
2144 GCTAAGC
BspDI 1526 ATCGAT AT^CGAT
4269 ATCGAT
BspLU11I 567 ACATGT A^CATGT
5051 ACATGT
BsrFI 50 GCCGGC [GA]^CCGG[CT]
3538 GCCGGC
BssSI 1541 CTCGTG CACGAG(-5/-1)
3970 CTCGTG
CelII 1789 GCTCAGC GC^T[ACGT]AGC
2144 GCTAAGC
Cfr10I 50 GCCGGC [GA]^CCGG[CT]
3538 GCCGGC
ClaI 1526 ATCGAT AT^CGAT
4269 ATCGAT
DsaI 600 CCATGG C^C[GA][CT]GG
807 CCACGG
HaeII 9 GGCGCC [GA]GCGC^[CT]
1090 AGCGCC
NaeI 50 GCCGGC GCC^GGC
3538 GCCGGC
NgoMIV 50 GCCGGC G^CCGGC
3538 GCCGGC
PmaCI 1999 CACGTG CAC^GTG
3011 CACGTG
PmlI 1999 CACGTG CAC^GTG
3011 CACGTG
PpuMI 195 GGGTCCT [GA]G^G[AT]CC[CT]
1404 GGGACCT
PstI 4675 CTGCAG CTGCA^G
4690 CTGCAG
SmlI 3393 CTCAAG C^T[CT][GA]AG
3581 CTTGAG
SpeI 3301 ACTAGT A^CTAGT
4480 ACTAGT
XmnI 2592 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2835 GAAGCATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 128 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI AocI ApaI
ApoI AspHI AspI AvaI AvaII
BalI BanII BbsI BbvCI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaAI BsaBI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BspMI BsrDI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MamI MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflFI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Tth111I
TthHB8I XcmI XhoII