##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AclI AgeI AhdI
AscI AseI AsnI Asp718I AspEI
BciVI BsiEI BsiWI BsmBI BspDI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstZ17I ClaI CpoI DraI
DraIII EagI Eam1105I EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT22I FseI
HindIII HpaI KpnI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PshBI Psp1406I PvuI
RsrII ScaI SexAI SfuI SgrAI
SmaI SnaBI SpeI SphI SspI
SwaI XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 3759 GTCGAC GT^[AC][GT]AC
AccIII 3172 TCCGGA T^CCGGA
AflIII 1278 ACGTGT A^C[GA][CT]GT
Alw44I 992 GTGCAC G^TGCAC
Aor51HI 2835 AGCGCT AGC^GCT
ApaLI 992 GTGCAC G^TGCAC
Asp700I 2659 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 2219 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2124 TGCGCA TGC^GCA
AvrII 2594 CCTAGG C^CTAGG
BbsI 398 GAAGAC GAAGAC(2/6)
BclI 2643 TGATCA T^GATCA
BglII 2334 AGATCT A^GATCT
BlnI 2594 CCTAGG C^CTAGG
BpuAI 398 GAAGAC GAAGAC(2/6)
BsaBI 2999 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 3172 TCCGGA T^CCGGA
BsmI 2100 GCATTC GAATGC(1/-1)
Bsp1407I 4533 TGTACA T^GTACA
BspEI 3172 TCCGGA T^CCGGA
BsrGI 4533 TGTACA T^GTACA
DrdI 92 GACGGAGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2835 AGCGCT AGC^GCT
FbaI 2643 TGATCA T^GATCA
FspI 2124 TGCGCA TGC^GCA
KspI 25 CCGCGG CCGC^GG
MamI 2999 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 3172 TCCGGA T^CCGGA
NspI 194 GCATGT [GA]CATG^[CT]
PflFI 2219 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 4234 GACAAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2720 GAGCTC GAGCT^C
SacII 25 CCGCGG CCGC^GG
SalI 3759 GTCGAC G^TCGAC
SfiI 925 GGCCTGGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4533 TGTACA T^GTACA
Tth111I 2219 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1091 CCAGGTAGGCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2659 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1116 GGCGCC G[GA]^CG[CT]C
2503 GGCGCC
AflII 2440 CTTAAG C^TTAAG
4358 CTTAAG
AspHI 992 GTGCAC G[AT]GC[AT]^C
2720 GAGCTC
BalI 4182 TGGCCA TGG^CCA
4384 TGGCCA
BbeI 1116 GGCGCC GGCGC^C
2503 GGCGCC
BbrPI 1277 CACGTG CAC^GTG
2066 CACGTG
BcgI 550 CGAGCTTAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3751 GCAAAGAGGTCG
BfrI 2440 CTTAAG C^TTAAG
4358 CTTAAG
BsaHI 1116 GGCGCC G[GA]^CG[CT]C
2503 GGCGCC
BsaWI 2899 ACCGGA [AT]^CCGG[AT]
3172 TCCGGA
BsiHKAI 992 GTGCAC G[AT]GC[AT]^C
2720 GAGCTC
BspHI 2001 TCATGA T^CATGA
4695 TCATGA
BsrDI 2449 CATTGC GCAATG(2/0)
3766 GCAATG
BssSI 3959 CACGAG CACGAG(-5/-1)
3135 CTCGTG
EaeI 4182 TGGCCA [CT]^GGCC[GA]
4384 TGGCCA
Hin1I 1116 GGCGCC G[GA]^CG[CT]C
2503 GGCGCC
HincII 6 GTTGAC GT[CT]^[GA]AC
3759 GTCGAC
HindII 6 GTTGAC GT[CT]^[GA]AC
3759 GTCGAC
KasI 1116 GGCGCC G^GCGCC
2503 GGCGCC
MluNI 4182 TGGCCA TGG^CCA
4384 TGGCCA
MscI 4182 TGGCCA TGG^CCA
4384 TGGCCA
NarI 1116 GGCGCC GG^CGCC
2503 GGCGCC
PaeR7I 637 CTCGAG C^TCGAG
4101 CTCGAG
PmaCI 1277 CACGTG CAC^GTG
2066 CACGTG
PmlI 1277 CACGTG CAC^GTG
2066 CACGTG
RcaI 2001 TCATGA T^CATGA
4695 TCATGA
SapI 413 GAAGAGC GCTCTTC(1/4)
36 GCTCTTC
SfoI 1116 GGCGCC GGC^GCC
2503 GGCGCC
XhoI 637 CTCGAG C^TCGAG
4101 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApaI ApoI
AvaI AvaII BamHI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I SbfI ScrFI
SfaNI SfcI SmlI Sse8387I StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII