##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AccIII AclI AflII AflIII AgeI
Aor51HI AscI AvrII BbeI BbrPI
BcgI BciVI BfrI BglI BlnI
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI BspEI BspLU11I BsrBI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
CpoI DraIII EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT22I FseI
HaeII KasI KspI MamI MfeI
MluI MroI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI Psp1406I
PvuI RsrII SacI SacII SbfI
ScaI SfiI SfoI SfuI SmlI
SphI Sse8387I SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AatII 2303 GACGTC GACGT^C
Acc65I 869 GGTACC G^GTACC
AccII 20 CGCG CG^CG
AcyI 2303 GACGTC G[GA]^CG[CT]C
AhdI 830 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2595 GTGCAC G^TGCAC
AocI 299 CCTCAGG CC^T[ACGT]AGG
ApaLI 2595 GTGCAC G^TGCAC
AseI 1322 ATTAAT AT^TAAT
AsnI 1322 ATTAAT AT^TAAT
Asp718I 869 GGTACC G^GTACC
AspEI 830 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 695 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2334 TGCGCA TGC^GCA
BalI 580 TGGCCA TGG^CCA
BamHI 1213 GGATCC G^GATCC
BbvCI 1616 GCTGAGG CCTCAGC(-5/-2)
BglII 3145 AGATCT A^GATCT
BlpI 854 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 854 GCTCAGC GC^T[ACGT]AGC
BsaAI 3004 TACGTA [CT]AC^GT[GA]
BsaHI 2303 GACGTC G[GA]^CG[CT]C
BsaWI 1201 TCCGGT [AT]^CCGG[AT]
BsmI 3098 GCATTC GAATGC(1/-1)
BspDI 2339 ATCGAT AT^CGAT
BssSI 2090 CTCGTG CACGAG(-5/-1)
BstUI 20 CGCG CG^CG
BstXI 942 CCAACGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 299 CCTCAGG CC^T[ACGT]AGG
CelII 854 GCTCAGC GC^T[ACGT]AGC
ClaI 2339 ATCGAT AT^CGAT
DraI 2222 TTTAAA TTT^AAA
DrdI 2345 GACCAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 830 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 299 CCTCAGG CC^T[ACGT]AGG
EcoRI 95 GAATTC G^AATTC
FspI 2334 TGCGCA TGC^GCA
HgaI 3201 GCGTC GACGC(5/10)
Hin1I 2303 GACGTC G[GA]^CG[CT]C
HpaI 1574 GTTAAC GTT^AAC
KpnI 869 GGTACC GGTAC^C
MluNI 580 TGGCCA TGG^CCA
MscI 580 TGGCCA TGG^CCA
MslI 1462 CACTAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 846 CAGCTG C[AC]G^C[GT]G
MvnI 20 CGCG CG^CG
NcoI 45 CCATGG C^CATGG
NspI 642 ACATGC [GA]CATG^[CT]
PflFI 695 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1810 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1322 ATTAAT AT^TAAT
PvuII 846 CAGCTG CAG^CTG
SalI 1602 GTCGAC G^TCGAC
SexAI 2373 ACCAGGT A^CC[AT]GGT
SgrAI 1110 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 782 CCCGGG CCC^GGG
SnaBI 3004 TACGTA TAC^GTA
SpeI 2910 ACTAGT A^CTAGT
StuI 1406 AGGCCT AGG^CCT
Tth111I 695 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 782 CCCGGG C^CCGGG
XmaI 782 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 778 GGGCCC GGGCC^C
1795 GGGCCC
AspHI 735 GAGCAC G[AT]GC[AT]^C
2595 GTGCAC
AvaII 982 GGACC G^G[AT]CC
2431 GGACC
BbsI 2122 GAAGAC GAAGAC(2/6)
2306 GTCTTC
BclI 1296 TGATCA T^GATCA
2361 TGATCA
BpuAI 2122 GAAGAC GAAGAC(2/6)
2306 GTCTTC
BseRI 117 CTCCTC GAGGAG(10/8)
1105 CTCCTC
BsiHKAI 735 GAGCAC G[AT]GC[AT]^C
2595 GTGCAC
BspHI 1084 TCATGA T^CATGA
1651 TCATGA
BspMI 2433 ACCTGC ACCTGC(4/8)
1174 GCAGGT
BsrDI 2747 CATTGC GCAATG(2/0)
3012 CATTGC
BsrFI 1111 GCCGGT [GA]^CCGG[CT]
1853 ACCGGC
BstAPI 617 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1340 GCATTGGTTGC
BstYI 1213 GGATCC [GA]^GATC[CT]
3145 AGATCT
Cfr10I 1111 GCCGGT [GA]^CCGG[CT]
1853 ACCGGC
EcoRV 1946 GATATC GAT^ATC
3227 GATATC
FbaI 1296 TGATCA T^GATCA
2361 TGATCA
HindIII 1676 AAGCTT A^AGCTT
2141 AAGCTT
MflI 1213 GGATCC [GA]^GATC[CT]
3145 AGATCT
PflMI 143 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1062 CCAAGTCCTGG
RcaI 1084 TCATGA T^CATGA
1651 TCATGA
SapI 1745 GAAGAGC GCTCTTC(1/4)
3185 GCTCTTC
SspI 2153 AATATT AAT^ATT
3024 AATATT
Van91I 143 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1062 CCAAGTCCTGG
XcmI 561 CCACAGAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1149 CCATATCTCCCCTGG
XhoII 1213 GGATCC [GA]^GATC[CT]
3145 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AvaI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmnI