##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
AocI Aor51HI ApaI AscI AseI
AsnI AspI AviII AvrII BbeI
BbrPI BcnI BlnI BsaAI BsaHI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BsrBI BsrFI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraIII
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I FauI FseI FspI
HapII Hin1I HpaI HpaII KasI
KspI MluI MroI MspI NaeI
NarI NciI NgoMIV NotI NruI
NspV PacI PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I StuI
Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 3405 GGTACC G^GTACC
AccII 4375 CGCG CG^CG
AflII 2730 CTTAAG C^TTAAG
AhdI 2169 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2213 GTGCAC G^TGCAC
ApaLI 2213 GTGCAC G^TGCAC
Asp718I 3405 GGTACC G^GTACC
AspEI 2169 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 2381 TGGCCA TGG^CCA
BamHI 3969 GGATCC G^GATCC
BanI 3405 GGTACC G^G[CT][GA]CC
BclI 3842 TGATCA T^GATCA
BfrI 2730 CTTAAG C^TTAAG
BsaBI 2637 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 4252 CGTCTC CGTCTC(1/5)
BspHI 3370 TCATGA T^CATGA
BspMI 2173 GCAGGT ACCTGC(4/8)
BsrDI 3296 CATTGC GCAATG(2/0)
BstUI 4375 CGCG CG^CG
CfoI 2076 GCGC GCG^C
EaeI 2381 TGGCCA [CT]^GGCC[GA]
Eam1105I 2169 GACTGCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4319 GAATTC G^AATTC
EcoRV 2826 GATATC GAT^ATC
FbaI 3842 TGATCA T^GATCA
HaeII 2075 AGCGCC [GA]GCGC^[CT]
HgaI 4376 GCGTC GACGC(5/10)
HhaI 2076 GCGC GCG^C
HinP1I 2076 GCGC G^CGC
HincII 3260 GTTGAC GT[CT]^[GA]AC
HindII 3260 GTTGAC GT[CT]^[GA]AC
HindIII 3981 AAGCTT A^AGCTT
KpnI 3405 GGTACC GGTAC^C
MamI 2637 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2381 TGGCCA TGG^CCA
MscI 2381 TGGCCA TGG^CCA
MvnI 4375 CGCG CG^CG
NcoI 4139 CCATGG C^CATGG
NheI 2870 GCTAGC G^CTAGC
PaeR7I 3661 CTCGAG C^TCGAG
PflMI 3420 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 3370 TCATGA T^CATGA
SacI 2103 GAGCTC GAGCT^C
SapI 2608 GCTCTTC GCTCTTC(1/4)
SpeI 4792 ACTAGT A^CTAGT
SwaI 4727 ATTTAAAT ATTT^AAAT
Van91I 3420 CCACTCATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 3661 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 1769 GTCTAC GT^[AC][GT]AC
4578 GTCTAC
AciI 3791 CCGC CCGC(-3/-1)
4374 CCGC
BcgI 2219 CGACACATCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3723 CGAAGGGAGTGC
BglI 666 GCCCACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1616 GCCTAAAAGGC
BglII 896 AGATCT A^GATCT
3052 AGATCT
BpmI 4110 CTCCAG CTGGAG(16/14)
3340 CTGGAG
BseRI 3898 CTCCTC GAGGAG(10/8)
4348 CTCCTC
Bsp1407I 270 TGTACA T^GTACA
1929 TGTACA
BspLU11I 267 ACATGT A^CATGT
3716 ACATGT
BsrGI 270 TGTACA T^GTACA
1929 TGTACA
DraII 104 GGGACCC [GA]G^G[ACGT]CC[CT]
4689 AGGTCCT
DrdI 732 GACCTCAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3857 GACTAGACAGTC
DsaI 1994 CCACGG C^C[GA][CT]GG
4139 CCATGG
EcoO109I 104 GGGACCC [GA]G^G[ACGT]CC[CT]
4689 AGGTCCT
EcoT22I 2983 ATGCAT ATGCA^T
4434 ATGCAT
NdeI 1568 CATATG CA^TATG
2980 CATATG
NsiI 2983 ATGCAT ATGCA^T
4434 ATGCAT
PpuMI 104 GGGACCC [GA]G^G[AT]CC[CT]
4689 AGGTCCT
PstI 2171 CTGCAG CTGCA^G
3012 CTGCAG
SspBI 270 TGTACA T^GTACA
1929 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AcsI AfaI AflIII AluI AlwI
AlwNI ApoI Asp700I AspHI AvaI
AvaII BanII BbsI BbvCI BbvI
BciVI BfaI BlpI BmyI Bpu1102I
BpuAI BsaI BsaJI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BsrI BssKI BstAPI
BstF5I BstNI BstXI BstYI Cac8I
CelII Cfr13I DdeI DpnII DraI
EarI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MfeI MflI MnlI MseI MslI
MspA1I MunI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScaI ScrFI
SfaNI SfcI SmlI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmnI