##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AgeI AhdI Aor51HI
ApaI AscI Asp718I AspEI AspI
AviII BamHI BanI BbeI BfrI
BglII BsaBI BsaHI BsiWI BsmBI
BspDI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I ClaI
CpoI Eam1105I Eco47III EcoO65I EcoRI
EcoRV FseI FspI HaeII Hin1I
KasI KpnI KspI MamI MluI
MvnI NarI NdeI NruI NspV
PflFI PflMI PinAI PmeI Psp1406I
PvuI RsrII SacII SalI SbfI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I Tth111I Van91I
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AocI 4713 CCTTAGG CC^T[ACGT]AGG
AseI 3480 ATTAAT AT^TAAT
AsnI 3480 ATTAAT AT^TAAT
AvrII 4091 CCTAGG C^CTAGG
BalI 5081 TGGCCA TGG^CCA
BcgI 4943 GCAGATTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 1710 GCCGAGTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 4091 CCTAGG C^CTAGG
BsiEI 57 CGGCCG CG[GA][CT]^CG
BsrFI 1142 GCCGGC [GA]^CCGG[CT]
Bsu36I 4713 CCTTAGG CC^T[ACGT]AGG
Cfr10I 1142 GCCGGC [GA]^CCGG[CT]
DrdI 4621 GACCAGTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 57 CGGCCG C^GGCCG
EarI 4664 GAAGAG CTCTTC(1/4)
EclXI 57 CGGCCG C^GGCCG
Eco52I 57 CGGCCG C^GGCCG
Eco81I 4713 CCTTAGG CC^T[ACGT]AGG
EcoNI 4713 CCTTAGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 5170 ATGCAT ATGCA^T
HgaI 1524 GCGTC GACGC(5/10)
HindIII 4819 AAGCTT A^AGCTT
HpaI 1778 GTTAAC GTT^AAC
Ksp632I 4664 GAAGAG CTCTTC(1/4)
MluNI 5081 TGGCCA TGG^CCA
MscI 5081 TGGCCA TGG^CCA
NaeI 1142 GCCGGC GCC^GGC
NgoMIV 1142 GCCGGC G^CCGGC
NheI 4216 GCTAGC G^CTAGC
NotI 56 GCGGCCGC GC^GGCCGC
NsiI 5170 ATGCAT ATGCA^T
PacI 2378 TTAATTAA TTAAT^TAA
PaeR7I 616 CTCGAG C^TCGAG
PshAI 3600 GACCTTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3480 ATTAAT AT^TAAT
SacI 4703 GAGCTC GAGCT^C
SapI 4664 GAAGAGC GCTCTTC(1/4)
SexAI 4406 ACCTGGT A^CC[AT]GGT
SmlI 616 CTCGAG C^T[CT][GA]AG
SspI 4034 AATATT AAT^ATT
StuI 1248 AGGCCT AGG^CCT
SwaI 4995 ATTTAAAT ATTT^AAAT
XbaI 810 TCTAGA T^CTAGA
XhoI 616 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccIII 28 TCCGGA T^CCGGA
717 TCCGGA
AlwNI 2648 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2903 CAGAAGCTG
AvaI 9 CCCGAG C^[CT]CG[GA]G
616 CTCGAG
BbvCI 66 CCTCAGC CCTCAGC(-5/-2)
1215 GCTGAGG
BciVI 2534 GGATAC GTATCC(6/5)
1570 GTATCC
BclI 246 TGATCA T^GATCA
3457 TGATCA
BlpI 2471 GCTTAGC GC^T[ACGT]AGC
4132 GCTGAGC
Bpu1102I 2471 GCTTAGC GC^T[ACGT]AGC
4132 GCTGAGC
BsaI 619 GAGACC GGTCTC(1/5)
1544 GGTCTC
BseAI 28 TCCGGA T^CCGGA
717 TCCGGA
BsmI 983 GCATTC GAATGC(1/-1)
4652 GCATTC
BsoBI 9 CCCGAG C^[CT]CG[GA]G
616 CTCGAG
BspEI 28 TCCGGA T^CCGGA
717 TCCGGA
BspHI 2914 TCATGA T^CATGA
4635 TCATGA
BsrBI 79 GAGCGG CCGCTC(-3/-3)
86 GAGCGG
BsrDI 1536 GCAATG GCAATG(2/0)
1641 GCAATG
BssSI 1165 CACGAG CACGAG(-5/-1)
4951 CTCGTG
BstXI 695 CCACTAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2097 CCATGCTGGTGG
BstYI 444 GGATCT [GA]^GATC[CT]
2613 GGATCT
CelII 2471 GCTTAGC GC^T[ACGT]AGC
4132 GCTGAGC
CfoI 1996 GCGC GCG^C
3842 GCGC
DraI 3935 TTTAAA TTT^AAA
4996 TTTAAA
DraIII 3087 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
3375 CACTGAGTG
EaeI 57 CGGCCG [CT]^GGCC[GA]
5081 TGGCCA
FbaI 246 TGATCA T^GATCA
3457 TGATCA
HhaI 1996 GCGC GCG^C
3842 GCGC
HinP1I 1996 GCGC G^CGC
3842 GCGC
MfeI 1908 CAATTG C^AATTG
3668 CAATTG
MflI 444 GGATCT [GA]^GATC[CT]
2613 GGATCT
MroI 28 TCCGGA T^CCGGA
717 TCCGGA
MunI 1908 CAATTG C^AATTG
3668 CAATTG
NcoI 1259 CCATGG C^CATGG
3527 CCATGG
RcaI 2914 TCATGA T^CATGA
4635 TCATGA
ScaI 632 AGTACT AGT^ACT
4588 AGTACT
XcmI 1226 CCATGCTGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3319 CCATGTTCCAAGTGG
XhoII 444 GGATCT [GA]^GATC[CT]
2613 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspHI AvaII BanII BbrPI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BspLU11I BspMI
BsrGI BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PmaCI PmlI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SphI
SspBI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XmnI