##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII AclI AflII AflIII Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I BbeI BbrPI BciVI BfrI
BglII BsaAI BsaBI BsiWI BspDI
BspLU11I BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoRV FseI KasI
MamI MfeI MluI MunI NaeI
NarI NgoMIV NotI NspV PacI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI ScaI SexAI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI XbaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
Acc65I 4511 GGTACC G^GTACC
AccI 2325 GTCTAC GT^[AC][GT]AC
AccIII 776 TCCGGA T^CCGGA
AgeI 27 ACCGGT A^CCGGT
Asp718I 4511 GGTACC G^GTACC
AspI 44 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2509 TGCGCA TGC^GCA
AvrII 1448 CCTAGG C^CTAGG
BcgI 2339 GCACAAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1684 TGATCA T^GATCA
BlnI 1448 CCTAGG C^CTAGG
BseAI 776 TCCGGA T^CCGGA
BsiEI 43 CGACCG CG[GA][CT]^CG
BsmBI 1511 CGTCTC CGTCTC(1/5)
BsmI 2444 GAATGC GAATGC(1/-1)
BspEI 776 TCCGGA T^CCGGA
BsrBI 402 GAGCGG CCGCTC(-3/-3)
BstEII 3975 GGTGACC G^GT[ACGT]ACC
BstPI 3975 GGTGACC G^GT[ACGT]ACC
BstXI 3739 CCACGTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 41 GACGACCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 3975 GGTGACC G^GT[ACGT]ACC
EcoT22I 4835 ATGCAT ATGCA^T
FbaI 1684 TGATCA T^GATCA
FspI 2509 TGCGCA TGC^GCA
KpnI 4511 GGTACC GGTAC^C
KspI 234 CCGCGG CCGC^GG
MroI 776 TCCGGA T^CCGGA
NheI 3140 GCTAGC G^CTAGC
NsiI 4835 ATGCAT ATGCA^T
NspI 1371 ACATGC [GA]CATG^[CT]
PaeR7I 65 CTCGAG C^TCGAG
PflFI 44 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 27 ACCGGT A^CCGGT
PshAI 1751 GACGCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacII 234 CCGCGG CCGC^GG
SbfI 842 CCTGCAGG CCTGCA^GG
Sse8387I 842 CCTGCAGG CCTGCA^GG
Tth111I 44 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 65 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 1509 GGCGTC G[GA]^CG[CT]C
1751 GACGCC
AhdI 2087 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4469 GACCCAGGGTC
ApaI 92 GGGCCC GGGCC^C
2727 GGGCCC
AspEI 2087 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4469 GACCCAGGGTC
BamHI 737 GGATCC G^GATCC
1462 GGATCC
BlpI 2999 GCTGAGC GC^T[ACGT]AGC
4747 GCTTAGC
Bpu1102I 2999 GCTGAGC GC^T[ACGT]AGC
4747 GCTTAGC
BsaHI 1509 GGCGTC G[GA]^CG[CT]C
1751 GACGCC
BsrDI 4088 CATTGC GCAATG(2/0)
3025 GCAATG
BsrFI 10 GCCGGT [GA]^CCGG[CT]
27 ACCGGT
BssHII 267 GCGCGC G^CGCGC
269 GCGCGC
CelII 2999 GCTGAGC GC^T[ACGT]AGC
4747 GCTTAGC
Cfr10I 10 GCCGGT [GA]^CCGG[CT]
27 ACCGGT
DraIII 2669 CACGCAGTG CAC[ACGT][ACGT][ACGT]^GTG
4671 CACTAGGTG
Eam1105I 2087 GACTTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4469 GACCCAGGGTC
EcoRI 1607 GAATTC G^AATTC
3174 GAATTC
HaeII 385 AGCGCC [GA]GCGC^[CT]
2485 AGCGCC
Hin1I 1509 GGCGTC G[GA]^CG[CT]C
1751 GACGCC
HindIII 3426 AAGCTT A^AGCTT
4936 AAGCTT
HpaI 2004 GTTAAC GTT^AAC
2370 GTTAAC
MslI 1420 CACCTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3740 CACGTCAGTG
NcoI 430 CCATGG C^CATGG
1364 CCATGG
NdeI 442 CATATG CA^TATG
1399 CATATG
NruI 167 TCGCGA TCG^CGA
201 TCGCGA
SfiI 2042 GGCCAGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4772 GGCCTGGGTGGCC
StuI 2050 AGGCCT AGG^CCT
3956 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 127 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I Bsp1407I BspHI BspMI BsrGI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PpuMI PstI
PvuII RcaI RsaI SacI SapI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI SspBI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmaCI XmaI