##################################
# NO CUT ENZYMES ( 79 enzymes) #
##################################
AatII AclI AgeI AhdI AocI
Aor51HI AscI AseI AsnI AspEI
AspI AviII AvrII BalI BamHI
BcgI BciVI BlnI BsiEI BsiWI
BsmBI BspDI BsrBI BsrDI BsrFI
BssHII Bst1107I BstZ17I Bsu36I Cfr10I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI FseI FspI HgaI KspI
MluI MluNI MscI NaeI NgoMIV
NotI NruI PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SapI SbfI SfiI SgrAI SmaI
SnaBI SpeI Sse8387I StuI SwaI
Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 1581 GGTACC G^GTACC
AccI 2758 GTCTAC GT^[AC][GT]AC
AccIII 1795 TCCGGA T^CCGGA
AcyI 116 GGCGCC G[GA]^CG[CT]C
AflIII 2897 ACATGT A^C[GA][CT]GT
Alw44I 374 GTGCAC G^TGCAC
ApaI 1113 GGGCCC GGGCC^C
ApaLI 374 GTGCAC G^TGCAC
Asp700I 4225 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1581 GGTACC G^GTACC
AvaI 1692 CTCGGG C^[CT]CG[GA]G
BbeI 116 GGCGCC GGCGC^C
BbrPI 3096 CACGTG CAC^GTG
BbvCI 4275 GCTGAGG CCTCAGC(-5/-2)
BglI 3856 GCCTAATTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 1225 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 116 GGCGCC G[GA]^CG[CT]C
BsaI 4157 GGTCTC GGTCTC(1/5)
BsaWI 1795 TCCGGA [AT]^CCGG[AT]
BseAI 1795 TCCGGA T^CCGGA
BsoBI 1692 CTCGGG C^[CT]CG[GA]G
BspEI 1795 TCCGGA T^CCGGA
BspHI 3376 TCATGA T^CATGA
BspLU11I 2897 ACATGT A^CATGT
BspMI 1178 GCAGGT ACCTGC(4/8)
BssSI 4187 CACGAG CACGAG(-5/-1)
BstEII 722 GGTGACC G^GT[ACGT]ACC
BstPI 722 GGTGACC G^GT[ACGT]ACC
DraI 4214 TTTAAA TTT^AAA
DrdI 3291 GACTTATTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 722 GGTGACC G^GT[ACGT]ACC
HaeII 116 GGCGCC [GA]GCGC^[CT]
Hin1I 116 GGCGCC G[GA]^CG[CT]C
KasI 116 GGCGCC G^GCGCC
KpnI 1581 GGTACC GGTAC^C
MamI 1225 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1795 TCCGGA T^CCGGA
NarI 116 GGCGCC GG^CGCC
NcoI 945 CCATGG C^CATGG
NheI 3694 GCTAGC G^CTAGC
PflMI 3326 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3096 CACGTG CAC^GTG
PmlI 3096 CACGTG CAC^GTG
PpuMI 4106 AGGACCC [GA]G^G[AT]CC[CT]
RcaI 3376 TCATGA T^CATGA
SexAI 637 ACCTGGT A^CC[AT]GGT
SfoI 116 GGCGCC GGC^GCC
SphI 973 GCATGC GCATG^C
Van91I 3326 CCAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2527 TCTAGA T^CTAGA
XcmI 3302 CCAGAATATGCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 4225 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 50 CGCG CG^CG
139 CGCG
AflII 614 CTTAAG C^TTAAG
3993 CTTAAG
AspHI 374 GTGCAC G[AT]GC[AT]^C
1015 GAGCAC
BanII 9 GAGCCC G[GA]GC[CT]^C
1113 GGGCCC
BcnI 402 CCCGG CC^[GC]GG
3238 CCGGG
BfrI 614 CTTAAG C^TTAAG
3993 CTTAAG
BglII 2089 AGATCT A^GATCT
3436 AGATCT
BsaAI 155 TACGTG [CT]AC^GT[GA]
3096 CACGTG
BsiHKAI 374 GTGCAC G[AT]GC[AT]^C
1015 GAGCAC
BstUI 50 CGCG CG^CG
139 CGCG
EcoRV 1106 GATATC GAT^ATC
1685 GATATC
EcoT22I 971 ATGCAT ATGCA^T
3640 ATGCAT
HindIII 1302 AAGCTT A^AGCTT
3599 AAGCTT
HpaI 358 GTTAAC GTT^AAC
4630 GTTAAC
MaeII 156 ACGT A^CGT
3097 ACGT
MvnI 50 CGCG CG^CG
139 CGCG
NciI 402 CCCGG CC^[GC]GG
3238 CCGGG
NdeI 1702 CATATG CA^TATG
2943 CATATG
NsiI 971 ATGCAT ATGCA^T
3640 ATGCAT
PvuII 2108 CAGCTG CAG^CTG
3393 CAGCTG
ScaI 2066 AGTACT AGT^ACT
2293 AGTACT
SspI 448 AATATT AAT^ATT
1985 AATATT
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BbsI
BbvI BclI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
BsmI Bsp1286I Bsp1407I BsrGI BsrI
BssKI BstAPI BstBI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EarI EcoO109I EcoRI EcoRII EcoT14I
FauI FbaI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MfeI
MflI MnlI MseI MslI MspA1I
MspI MunI MvaI MwoI NdeII
NlaIII NlaIV NspI NspV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfuI SmlI SspBI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII