##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI AscI AseI AsnI
AspEI AspI AviII AvrII BbrPI
BcgI BclI BfrI BlnI BsaAI
BsaBI BsaWI BseAI BsiWI BsmBI
BspEI BssHII BstBI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FbaI FseI FspI HindIII
KspI MamI MfeI MroI MunI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI Sse8387I SwaI Tth111I
XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AcyI 628 GGCGCC G[GA]^CG[CT]C
ApaI 73 GGGCCC GGGCC^C
Asp700I 2360 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 5087 GGATCC G^GATCC
BbeI 628 GGCGCC GGCGC^C
BglI 4376 GCCTTAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1898 AGATCT A^GATCT
BsaHI 628 GGCGCC G[GA]^CG[CT]C
BsaI 395 GAGACC GGTCTC(1/5)
BsmI 1680 GCATTC GAATGC(1/-1)
Bsp1407I 4990 TGTACA T^GTACA
BspDI 1858 ATCGAT AT^CGAT
BspHI 2748 TCATGA T^CATGA
BspLU11I 964 ACATGT A^CATGT
BsrBI 148 GAGCGG CCGCTC(-3/-3)
BsrGI 4990 TGTACA T^GTACA
BssSI 3140 CTCGTG CACGAG(-5/-1)
Bst1107I 1819 GTATAC GTA^TAC
BstEII 660 GGTGACC G^GT[ACGT]ACC
BstPI 660 GGTGACC G^GT[ACGT]ACC
BstXI 875 CCAGCGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1819 GTATAC GTA^TAC
ClaI 1858 ATCGAT AT^CGAT
EcoO65I 660 GGTGACC G^GT[ACGT]ACC
EcoT22I 642 ATGCAT ATGCA^T
Hin1I 628 GGCGCC G[GA]^CG[CT]C
KasI 628 GGCGCC G^GCGCC
MluI 903 ACGCGT A^CGCGT
NaeI 1349 GCCGGC GCC^GGC
NarI 628 GGCGCC GG^CGCC
NdeI 4504 CATATG CA^TATG
NgoMIV 1349 GCCGGC G^CCGGC
NheI 4310 GCTAGC G^CTAGC
NsiI 642 ATGCAT ATGCA^T
PmeI 4106 GTTTAAAC GTTT^AAAC
RcaI 2748 TCATGA T^CATGA
SexAI 2412 ACCTGGT A^CC[AT]GGT
SfoI 628 GGCGCC GGC^GCC
SmaI 3994 CCCGGG CCC^GGG
SpeI 272 ACTAGT A^CTAGT
SspBI 4990 TGTACA T^GTACA
SspI 5001 AATATT AAT^ATT
XbaI 2060 TCTAGA T^CTAGA
XmaCI 3994 CCCGGG C^CCGGG
XmaI 3994 CCCGGG C^CCGGG
XmnI 2360 GAATCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 2263 GGTACC G^GTACC
2409 GGTACC
AccI 1819 GTATAC GT^[AC][GT]AC
3770 GTCTAC
AflIII 903 ACGCGT A^C[GA][CT]GT
964 ACATGT
Alw44I 834 GTGCAC G^TGCAC
1143 GTGCAC
ApaLI 834 GTGCAC G^TGCAC
1143 GTGCAC
Asp718I 2263 GGTACC G^GTACC
2409 GGTACC
BsiEI 5 CGACCG CG[GA][CT]^CG
788 CGACCG
BsrDI 2158 CATTGC GCAATG(2/0)
4578 GCAATG
DraIII 825 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
2352 CACACCGTG
EcoNI 1406 CCTCTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5047 CCTTAGGCAGG
EcoRI 4050 GAATTC G^AATTC
4847 GAATTC
HincII 2419 GTTAAC GT[CT]^[GA]AC
3330 GTTAAC
HindII 2419 GTTAAC GT[CT]^[GA]AC
3330 GTTAAC
HpaI 2419 GTTAAC GTT^AAC
3330 GTTAAC
KpnI 2263 GGTACC GGTAC^C
2409 GGTACC
PpuMI 3261 GGGACCT [GA]G^G[AT]CC[CT]
3275 GGGACCC
SapI 917 GAAGAGC GCTCTTC(1/4)
1008 GAAGAGC
ScaI 865 AGTACT AGT^ACT
3544 AGTACT
TaqI 1859 TCGA T^CGA
4048 TCGA
TthHB8I 1859 TCGA T^CGA
4048 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 121 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII