##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AclI AflII AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AvrII BamHI BciVI BclI BfrI
BglII BlnI BsiWI BspDI BsrDI
BstAPI BstBI ClaI CpoI Eam1105I
Eco47III EcoT22I FbaI FseI HincII
HindII HpaI KspI MfeI MluI
MunI NdeI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SwaI TfiI XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 147 GGTACC G^GTACC
AccIII 631 TCCGGA T^CCGGA
AcsI 411 GAATTC [GA]^AATT[CT]
AflIII 156 ACATGT A^C[GA][CT]GT
ApoI 411 GAATTC [GA]^AATT[CT]
Asp718I 147 GGTACC G^GTACC
AspI 1164 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1861 TGCGCA TGC^GCA
BbeI 35 GGCGCC GGCGC^C
BsaBI 1826 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 631 TCCGGA T^CCGGA
BspEI 631 TCCGGA T^CCGGA
BspHI 1420 TCATGA T^CATGA
BspLU11I 156 ACATGT A^CATGT
BssHII 64 GCGCGC G^CGCGC
Bst1107I 1839 GTATAC GTA^TAC
BstEII 2087 GGTAACC G^GT[ACGT]ACC
BstPI 2087 GGTAACC G^GT[ACGT]ACC
BstZ17I 1839 GTATAC GTA^TAC
DraI 4216 TTTAAA TTT^AAA
DraIII 3459 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2070 GACAGCCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 4097 CCTTGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2087 GGTAACC G^GT[ACGT]ACC
EcoRI 411 GAATTC G^AATTC
EcoRV 275 GATATC GAT^ATC
FspI 1861 TGCGCA TGC^GCA
HindIII 3857 AAGCTT A^AGCTT
KasI 35 GGCGCC G^GCGCC
KpnI 147 GGTACC GGTAC^C
MamI 1826 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 631 TCCGGA T^CCGGA
NarI 35 GGCGCC GG^CGCC
NheI 1589 GCTAGC G^CTAGC
PflFI 1164 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1420 TCATGA T^CATGA
SapI 2150 GCTCTTC GCTCTTC(1/4)
SfoI 35 GGCGCC GGC^GCC
SmlI 2727 CTTGAG C^T[CT][GA]AG
SspI 4203 AATATT AAT^ATT
Tth111I 1164 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AatII 2018 GACGTC GACGT^C
2358 GACGTC
Alw44I 1065 GTGCAC G^TGCAC
3472 GTGCAC
ApaLI 1065 GTGCAC G^TGCAC
3472 GTGCAC
BbrPI 639 CACGTG CAC^GTG
1062 CACGTG
BbsI 960 GTCTTC GAAGAC(2/6)
1721 GTCTTC
BpuAI 960 GTCTTC GAAGAC(2/6)
1721 GTCTTC
BsaAI 639 CACGTG [CT]AC^GT[GA]
1062 CACGTG
BsaWI 45 ACCGGA [AT]^CCGG[AT]
631 TCCGGA
BsmBI 2356 GAGACG CGTCTC(1/5)
2535 GAGACG
BsmI 609 GCATTC GAATGC(1/-1)
2677 GCATTC
BssSI 837 CACGAG CACGAG(-5/-1)
3569 CTCGTG
BstYI 628 AGATCC [GA]^GATC[CT]
3991 AGATCC
MflI 628 AGATCC [GA]^GATC[CT]
3991 AGATCC
NaeI 2251 GCCGGC GCC^GGC
3205 GCCGGC
NgoMIV 2251 GCCGGC G^CCGGC
3205 GCCGGC
PmaCI 639 CACGTG CAC^GTG
1062 CACGTG
PmlI 639 CACGTG CAC^GTG
1062 CACGTG
SacI 1095 GAGCTC GAGCT^C
4038 GAGCTC
SphI 1942 GCATGC GCATG^C
2602 GCATGC
TaqI 1162 TCGA T^CGA
2581 TCGA
TthHB8I 1162 TCGA T^CGA
2581 TCGA
XhoII 628 AGATCC [GA]^GATC[CT]
3991 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 129 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbvCI BbvI BcgI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaHI BsaI BsaJI BseRI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I Bsp1407I
BspMI BsrBI BsrFI BsrGI BsrI
BssKI BstF5I BstNI BstUI BstXI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI SspBI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XcmI XmaCI XmaI