##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII Acc65I AccI AclI AhdI
AocI Aor51HI ApaI AscI Asp718I
AspEI AviII AvrII BalI BbrPI
BbvCI BcgI BciVI BglI BglII
BlnI BsiWI BsmBI BspDI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraIII DrdI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FseI FspI
HpaI KpnI Ksp632I MfeI MluI
MluNI MscI MunI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PmaCI PmeI PmlI Psp1406I
PvuI SalI SapI SbfI ScaI
SexAI SfiI SfuI SnaBI SpeI
Sse8387I StuI SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccIII 1239 TCCGGA T^CCGGA
AcyI 45 GGCGCC G[GA]^CG[CT]C
AflII 3069 CTTAAG C^TTAAG
AgeI 442 ACCGGT A^CCGGT
Alw44I 446 GTGCAC G^TGCAC
ApaLI 446 GTGCAC G^TGCAC
Asp700I 1587 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 3345 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2675 GGATCC G^GATCC
BanI 45 GGCGCC G^G[CT][GA]CC
BbeI 45 GGCGCC GGCGC^C
BclI 2669 TGATCA T^GATCA
BfrI 3069 CTTAAG C^TTAAG
BlpI 110 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 110 GCTGAGC GC^T[ACGT]AGC
BsaAI 2686 TACGTG [CT]AC^GT[GA]
BsaHI 45 GGCGCC G[GA]^CG[CT]C
BseAI 1239 TCCGGA T^CCGGA
BsiEI 440 CGACCG CG[GA][CT]^CG
Bsp1407I 658 TGTACA T^GTACA
BspEI 1239 TCCGGA T^CCGGA
BspMI 100 ACCTGC ACCTGC(4/8)
BsrGI 658 TGTACA T^GTACA
BstXI 346 CCATGTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 110 GCTGAGC GC^T[ACGT]AGC
CpoI 331 CGGACCG CG^G[AT]CCG
EcoNI 1032 CCTTTCTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2669 TGATCA T^GATCA
HgaI 304 GACGC GACGC(5/10)
Hin1I 45 GGCGCC G[GA]^CG[CT]C
KasI 45 GGCGCC G^GCGCC
MroI 1239 TCCGGA T^CCGGA
NarI 45 GGCGCC GG^CGCC
NcoI 1805 CCATGG C^CATGG
PflFI 3345 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 442 ACCGGT A^CCGGT
PpuMI 11 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 3198 GACTTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2514 CTGCAG CTGCA^G
RsrII 331 CGGACCG CG^G[AT]CCG
SacI 1178 GAGCTC GAGCT^C
SfoI 45 GGCGCC GGC^GCC
SphI 3463 GCATGC GCATG^C
SspBI 658 TGTACA T^GTACA
Tth111I 3345 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1773 TCTAGA T^CTAGA
XmnI 1587 GAAGGTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 2182 ACATGT A^C[GA][CT]GT
2730 ACATGT
AseI 2429 ATTAAT AT^TAAT
2952 ATTAAT
AsnI 2429 ATTAAT AT^TAAT
2952 ATTAAT
BpmI 2089 CTCCAG CTGGAG(16/14)
2446 CTCCAG
BsaBI 2670 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3250 GATTAAAATC
BsaWI 442 ACCGGT [AT]^CCGG[AT]
1239 TCCGGA
BseRI 2349 CTCCTC GAGGAG(10/8)
1857 GAGGAG
BspHI 1595 TCATGA T^CATGA
2641 TCATGA
BspLU11I 2182 ACATGT A^CATGT
2730 ACATGT
BstYI 905 GGATCT [GA]^GATC[CT]
2675 GGATCC
DraII 11 GGGACCT [GA]G^G[ACGT]CC[CT]
1658 GGGGCCT
EcoO109I 11 GGGACCT [GA]G^G[ACGT]CC[CT]
1658 GGGGCCT
EcoRI 1384 GAATTC G^AATTC
3141 GAATTC
MamI 2670 GATCAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3250 GATTAAAATC
MflI 905 GGATCT [GA]^GATC[CT]
2675 GGATCC
NaeI 42 GCCGGC GCC^GGC
341 GCCGGC
NdeI 2302 CATATG CA^TATG
2407 CATATG
NgoMIV 42 GCCGGC G^CCGGC
341 GCCGGC
PshBI 2429 ATTAAT AT^TAAT
2952 ATTAAT
RcaI 1595 TCATGA T^CATGA
2641 TCATGA
SfcI 2514 CTGCAG C^T[GA][CT]AG
3076 CTGTAG
SgrAI 41 CGCCGGCG C[GA]^CCGG[CT]G
47 CGCCGGTG
SmaI 160 CCCGGG CCC^GGG
400 CCCGGG
SspI 2805 AATATT AAT^ATT
2960 AATATT
XcmI 346 CCATGTCGGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2107 CCAAAATAAAGGTGG
XhoII 905 GGATCT [GA]^GATC[CT]
2675 GGATCC
XmaCI 160 CCCGGG C^CCGGG
400 CCCGGG
XmaI 160 CCCGGG C^CCGGG
400 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanII BbsI BbvI BcnI
BfaI BmyI BpuAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DsaI EaeI EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HincII
HindII HindIII HinfI HpaII HphI
ItaI KspI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I