##################################
# NO CUT ENZYMES ( 61 enzymes) #
##################################
AatII Acc65I AccI AccIII AflII
AgeI AhdI Aor51HI AscI Asp718I
AspEI AviII BfrI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DrdI Eam1105I
Eco47III EcoRV FseI FspI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NcoI NotI NruI
NspV PacI PinAI PmeI PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SfuI SgrAI SnaBI
Sse8387I
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AclI 5703 AACGTT AA^CGTT
AcyI 51 GGCGCC G[GA]^CG[CT]C
Alw44I 548 GTGCAC G^TGCAC
ApaLI 548 GTGCAC G^TGCAC
AvrII 2413 CCTAGG C^CTAGG
BamHI 1036 GGATCC G^GATCC
BbeI 51 GGCGCC GGCGC^C
BbrPI 6094 CACGTG CAC^GTG
BcgI 2640 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1044 GGATAC GTATCC(6/5)
BclI 2329 TGATCA T^GATCA
BglI 2744 GCCAGGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2413 CCTAGG C^CTAGG
BlpI 4225 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 4225 GCTCAGC GC^T[ACGT]AGC
BsaHI 51 GGCGCC G[GA]^CG[CT]C
BsiEI 24 CGGCCG CG[GA][CT]^CG
BsrBI 90 GAGCGG CCGCTC(-3/-3)
BstXI 3180 CCAGTTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 4225 GCTCAGC GC^T[ACGT]AGC
DraIII 1792 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 252 CCGTGG C^C[GA][CT]GG
EagI 24 CGGCCG C^GGCCG
EclXI 24 CGGCCG C^GGCCG
Eco52I 24 CGGCCG C^GGCCG
EcoNI 1331 CCTGTTTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2329 TGATCA T^GATCA
Hin1I 51 GGCGCC G[GA]^CG[CT]C
HindIII 3479 AAGCTT A^AGCTT
KasI 51 GGCGCC G^GCGCC
NarI 51 GGCGCC GG^CGCC
NdeI 3253 CATATG CA^TATG
NheI 1961 GCTAGC G^CTAGC
PaeR7I 2145 CTCGAG C^TCGAG
PflMI 864 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 6094 CACGTG CAC^GTG
PmlI 6094 CACGTG CAC^GTG
PpuMI 2416 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2428 GACAGTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 5703 AACGTT AA^CGTT
PvuII 702 CAGCTG CAG^CTG
SapI 4230 GCTCTTC GCTCTTC(1/4)
SfiI 2743 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 51 GGCGCC GGC^GCC
SmaI 2628 CCCGGG CCC^GGG
SpeI 5108 ACTAGT A^CTAGT
SphI 3601 GCATGC GCATG^C
StuI 968 AGGCCT AGG^CCT
SwaI 2322 ATTTAAAT ATTT^AAAT
Van91I 864 CCAAGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2145 CTCGAG C^TCGAG
XmaCI 2628 CCCGGG C^CCGGG
XmaI 2628 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AocI 788 CCTCAGG CC^T[ACGT]AGG
2768 CCTCAGG
BalI 2742 TGGCCA TGG^CCA
4337 TGGCCA
BglII 684 AGATCT A^GATCT
4559 AGATCT
BsaAI 5811 TACGTG [CT]AC^GT[GA]
6094 CACGTG
BseRI 156 CTCCTC GAGGAG(10/8)
934 GAGGAG
BsgI 2423 GTGCAG GTGCAG(16/14)
2589 GTGCAG
BsrFI 123 GCCGGC [GA]^CCGG[CT]
135 GCCGGC
BssSI 1795 CTCGTG CACGAG(-5/-1)
1993 CTCGTG
Bsu36I 788 CCTCAGG CC^T[ACGT]AGG
2768 CCTCAGG
Cfr10I 123 GCCGGC [GA]^CCGG[CT]
135 GCCGGC
Eco81I 788 CCTCAGG CC^T[ACGT]AGG
2768 CCTCAGG
EcoRI 4517 GAATTC G^AATTC
4703 GAATTC
HaeII 51 GGCGCC [GA]GCGC^[CT]
2195 AGCGCC
HgaI 4738 GACGC GACGC(5/10)
5021 GCGTC
MluNI 2742 TGGCCA TGG^CCA
4337 TGGCCA
MscI 2742 TGGCCA TGG^CCA
4337 TGGCCA
MspA1I 702 CAGCTG C[AC]G^C[GT]G
1140 CAGCGG
NaeI 123 GCCGGC GCC^GGC
135 GCCGGC
NgoMIV 123 GCCGGC G^CCGGC
135 GCCGGC
PstI 2613 CTGCAG CTGCA^G
5348 CTGCAG
SexAI 313 ACCTGGT A^CC[AT]GGT
5117 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
AseI AsnI Asp700I AspHI AspI
AvaI AvaII BanI BanII BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I Bsp1407I BspLU11I BspMI BsrDI
BsrGI BsrI BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII EaeI EarI EcoO109I
EcoO65I EcoRII EcoT14I EcoT22I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NsiI NspI PflFI PleI PshBI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Tth111I TthHB8I XbaI
XcmI XhoII XmnI