##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AccI AclI AcyI
AflII AgeI AocI Aor51HI AscI
Asp718I AviII BbeI BbrPI BcgI
BciVI BclI BfrI BsaAI BsaHI
BsiEI BsiWI BsmBI BspDI BsrBI
BssHII BssSI Bst1107I BstBI BstZ17I
Bsu36I ClaI CpoI DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FbaI FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI MfeI MluI MunI
NarI NcoI NotI NruI NspV
PaeR7I PflMI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SacI
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
StuI SwaI Van91I XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccII 16 CGCG CG^CG
AccIII 98 TCCGGA T^CCGGA
AflIII 1367 ACATGT A^C[GA][CT]GT
AhdI 753 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 768 GTGCAC G^TGCAC
AlwNI 1435 CAGGGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 768 GTGCAC G^TGCAC
Asp700I 1156 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 753 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1094 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 2676 GGATCC G^GATCC
BanI 4044 GGCACC G^G[CT][GA]CC
BbvCI 4010 GCTGAGG CCTCAGC(-5/-2)
BlpI 1107 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1107 GCTTAGC GC^T[ACGT]AGC
BsaWI 98 TCCGGA [AT]^CCGG[AT]
BseAI 98 TCCGGA T^CCGGA
BspEI 98 TCCGGA T^CCGGA
BspLU11I 1367 ACATGT A^CATGT
BsrDI 3932 GCAATG GCAATG(2/0)
BstAPI 508 GCAGAAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1536 GGTGACC G^GT[ACGT]ACC
BstPI 1536 GGTGACC G^GT[ACGT]ACC
BstUI 16 CGCG CG^CG
BstXI 1999 CCAGATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1107 GCTTAGC GC^T[ACGT]AGC
Eam1105I 753 GACATGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1536 GGTGACC G^GT[ACGT]ACC
EcoT22I 404 ATGCAT ATGCA^T
HaeII 1751 GGCGCT [GA]GCGC^[CT]
HindIII 2490 AAGCTT A^AGCTT
KspI 15 CCGCGG CCGC^GG
MroI 98 TCCGGA T^CCGGA
MvnI 16 CGCG CG^CG
NsiI 404 ATGCAT ATGCA^T
PacI 4225 TTAATTAA TTAAT^TAA
PflFI 1094 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SacII 15 CCGCGG CCGC^GG
SapI 2993 GCTCTTC GCTCTTC(1/4)
SmaI 39 CCCGGG CCC^GGG
SpeI 3741 ACTAGT A^CTAGT
SphI 2840 GCATGC GCATG^C
SspI 3206 AATATT AAT^ATT
TaqI 2789 TCGA T^CGA
Tth111I 1094 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 2789 TCGA T^CGA
XbaI 2729 TCTAGA T^CTAGA
XcmI 1450 CCAAGCTAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 39 CCCGGG C^CCGGG
XmaI 39 CCCGGG C^CCGGG
XmnI 1156 GAACTTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
ApaI 36 GGGCCC GGGCC^C
142 GGGCCC
AvaI 39 CCCGGG C^[CT]CG[GA]G
698 CCCGAG
AvrII 1176 CCTAGG C^CTAGG
2427 CCTAGG
BalI 2771 TGGCCA TGG^CCA
3891 TGGCCA
BglI 14 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3536 GCCTTCATGGC
BglII 925 AGATCT A^GATCT
4220 AGATCT
BlnI 1176 CCTAGG C^CTAGG
2427 CCTAGG
BsaBI 866 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2375 GATGTAAATC
BsmI 2814 GAATGC GAATGC(1/-1)
1587 GCATTC
BsoBI 39 CCCGGG C^[CT]CG[GA]G
698 CCCGAG
Bsp1407I 264 TGTACA T^GTACA
2507 TGTACA
BsrFI 30 GCCGGC [GA]^CCGG[CT]
3911 GCCGGC
BsrGI 264 TGTACA T^GTACA
2507 TGTACA
CfoI 1752 GCGC GCG^C
3179 GCGC
Cfr10I 30 GCCGGC [GA]^CCGG[CT]
3911 GCCGGC
DsaI 15 CCGCGG C^C[GA][CT]GG
692 CCACGG
EaeI 2771 TGGCCA [CT]^GGCC[GA]
3891 TGGCCA
HhaI 1752 GCGC GCG^C
3179 GCGC
HinP1I 1752 GCGC G^CGC
3179 GCGC
MaeII 687 ACGT A^CGT
1295 ACGT
MamI 866 GATGATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2375 GATGTAAATC
MluNI 2771 TGGCCA TGG^CCA
3891 TGGCCA
MscI 2771 TGGCCA TGG^CCA
3891 TGGCCA
NaeI 30 GCCGGC GCC^GGC
3911 GCCGGC
NdeI 1258 CATATG CA^TATG
4188 CATATG
NgoMIV 30 GCCGGC G^CCGGC
3911 GCCGGC
NheI 1324 GCTAGC G^CTAGC
1454 GCTAGC
PpuMI 1244 AGGACCT [GA]G^G[AT]CC[CT]
1486 AGGACCT
PshAI 1439 GACTGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
3476 GACAGGAGTC
PvuII 3004 CAGCTG CAG^CTG
4096 CAGCTG
SspBI 264 TGTACA T^GTACA
2507 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AseI AsnI AspHI AvaII
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraI
DraII DraIII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PshBI
PstI RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII