##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AscI AseI AsnI BamHI BciVI
BclI BfrI BseAI BsiWI BsmI
Bsp1407I BspDI BspEI BsrGI BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
EarI EcoRV FbaI FseI HindIII
Ksp632I MluI MroI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I SalI
SapI SbfI ScaI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 2378 GGTACC G^GTACC
AccI 3543 GTAGAC GT^[AC][GT]AC
AhdI 1923 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 5093 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 2008 AGCGCT AGC^GCT
Asp700I 3047 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2378 GGTACC G^GTACC
AspEI 1923 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 4437 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2525 TGCGCA TGC^GCA
AvrII 3498 CCTAGG C^CTAGG
BbsI 784 GAAGAC GAAGAC(2/6)
BlnI 3498 CCTAGG C^CTAGG
BlpI 3620 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3620 GCTCAGC GC^T[ACGT]AGC
BpuAI 784 GAAGAC GAAGAC(2/6)
BsaBI 1445 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1519 ACCGGA [AT]^CCGG[AT]
BsgI 4311 GTGCAG GTGCAG(16/14)
BspHI 982 TCATGA T^CATGA
BspLU11I 3563 ACATGT A^CATGT
BstEII 1623 GGTGACC G^GT[ACGT]ACC
BstPI 1623 GGTGACC G^GT[ACGT]ACC
CelII 3620 GCTCAGC GC^T[ACGT]AGC
CpoI 1516 CGGACCG CG^G[AT]CCG
DrdI 1077 GACGATGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 91 CGGCCG C^GGCCG
Eam1105I 1923 GACTCCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 91 CGGCCG C^GGCCG
Eco47III 2008 AGCGCT AGC^GCT
Eco52I 91 CGGCCG C^GGCCG
EcoO65I 1623 GGTGACC G^GT[ACGT]ACC
FspI 2525 TGCGCA TGC^GCA
HpaI 3234 GTTAAC GTT^AAC
KpnI 2378 GGTACC GGTAC^C
MamI 1445 GATTACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4581 CAATTG C^AATTG
MunI 4581 CAATTG C^AATTG
PflFI 4437 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1152 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1889 CGATCG CGAT^CG
RcaI 982 TCATGA T^CATGA
RsrII 1516 CGGACCG CG^G[AT]CCG
SacI 4360 GAGCTC GAGCT^C
SexAI 3780 ACCAGGT A^CC[AT]GGT
SfiI 2900 GGCCCAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 599 GCATGC GCATG^C
StuI 3041 AGGCCT AGG^CCT
SwaI 5058 ATTTAAAT ATTT^AAAT
Tth111I 4437 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4364 TCTAGA T^CTAGA
XmnI 3047 GAAACATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Alw44I 1107 GTGCAC G^TGCAC
2450 GTGCAC
AlwI 3318 GGATC GGATC(4/5)
4045 GGATC
AocI 3193 CCTCAGG CC^T[ACGT]AGG
3451 CCTCAGG
ApaLI 1107 GTGCAC G^TGCAC
2450 GTGCAC
BbvCI 381 GCTGAGG CCTCAGC(-5/-2)
4168 GCTGAGG
BcgI 3625 GCAAAGGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4174 GCACAAGAATCG
BglII 316 AGATCT A^GATCT
4648 AGATCT
BsiEI 91 CGGCCG CG[GA][CT]^CG
1889 CGATCG
BsmBI 180 GAGACG CGTCTC(1/5)
540 GAGACG
BsrDI 1177 CATTGC GCAATG(2/0)
2624 GCAATG
BssHII 61 GCGCGC G^CGCGC
109 GCGCGC
BstYI 316 AGATCT [GA]^GATC[CT]
4648 AGATCT
Bsu36I 3193 CCTCAGG CC^T[ACGT]AGG
3451 CCTCAGG
DraI 3811 TTTAAA TTT^AAA
5059 TTTAAA
DraIII 1497 CACAATGTG CAC[ACGT][ACGT][ACGT]^GTG
3324 CACCACGTG
Eco81I 3193 CCTCAGG CC^T[ACGT]AGG
3451 CCTCAGG
EcoNI 1404 CCTGAATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3493 CCTCTCCTAGG
EcoRI 4736 GAATTC G^AATTC
4941 GAATTC
KspI 1986 CCGCGG CCGC^GG
2158 CCGCGG
MflI 316 AGATCT [GA]^GATC[CT]
4648 AGATCT
NaeI 2018 GCCGGC GCC^GGC
2331 GCCGGC
NgoMIV 2018 GCCGGC G^CCGGC
2331 GCCGGC
PstI 1234 CTGCAG CTGCA^G
2469 CTGCAG
PvuII 379 CAGCTG CAG^CTG
2929 CAGCTG
SacII 1986 CCGCGG CCGC^GG
2158 CCGCGG
SmaI 1718 CCCGGG CCC^GGG
1790 CCCGGG
TaqI 1018 TCGA T^CGA
4066 TCGA
TthHB8I 1018 TCGA T^CGA
4066 TCGA
XcmI 491 CCACCCTCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1658 CCACGATCACGATGG
XhoII 316 AGATCT [GA]^GATC[CT]
4648 AGATCT
XmaCI 1718 CCCGGG C^CCGGG
1790 CCCGGG
XmaI 1718 CCCGGG C^CCGGG
1790 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbeI BbrPI BbvI BcnI BfaI
BglI BmyI BpmI BsaAI BsaHI
BsaI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrFI BsrI BssKI
BstF5I BstNI BstUI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I EcoT22I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI KasI MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NcoI NdeII NlaIII NlaIV
NsiI NspI PflMI PleI PmaCI
PmlI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I