##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AclI AgeI Aor51HI ApaI
AscI AseI AsnI AspI BbeI
BbrPI BcgI BsiWI BspDI BssHII
Bst1107I BstBI BstZ17I ClaI CpoI
EagI EclXI Eco47III Eco52I EcoRV
EcoT22I FseI HincII HindII HpaI
KasI MfeI MluI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AccI 4395 GTAGAC GT^[AC][GT]AC
AccIII 3346 TCCGGA T^CCGGA
AflII 957 CTTAAG C^TTAAG
AhdI 5508 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 5508 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 4381 TGCGCA TGC^GCA
AvrII 5813 CCTAGG C^CTAGG
BbvCI 5384 CCTCAGC CCTCAGC(-5/-2)
BclI 5723 TGATCA T^GATCA
BfrI 957 CTTAAG C^TTAAG
BglI 1123 GCCTGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3970 AGATCT A^GATCT
BlnI 5813 CCTAGG C^CTAGG
BsaAI 4350 TACGTG [CT]AC^GT[GA]
BseAI 3346 TCCGGA T^CCGGA
BsiEI 2283 CGGTCG CG[GA][CT]^CG
BspEI 3346 TCCGGA T^CCGGA
BspLU11I 4495 ACATGT A^CATGT
BsrBI 66 GAGCGG CCGCTC(-3/-3)
BsrFI 3856 ACCGGC [GA]^CCGG[CT]
BssSI 3450 CACGAG CACGAG(-5/-1)
Cfr10I 3856 ACCGGC [GA]^CCGG[CT]
Eam1105I 5508 GACTCGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 4991 GAATTC G^AATTC
FbaI 5723 TGATCA T^GATCA
FspI 4381 TGCGCA TGC^GCA
HaeII 5330 GGCGCT [GA]GCGC^[CT]
HindIII 3264 AAGCTT A^AGCTT
KspI 4127 CCGCGG CCGC^GG
MroI 3346 TCCGGA T^CCGGA
PflMI 2669 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacII 4127 CCGCGG CCGC^GG
SapI 755 GCTCTTC GCTCTTC(1/4)
ScaI 2686 AGTACT AGT^ACT
SphI 5249 GCATGC GCATG^C
SspI 5911 AATATT AAT^ATT
Van91I 2669 CCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Acc65I 3703 GGTACC G^GTACC
5769 GGTACC
AcyI 5060 GACGCC G[GA]^CG[CT]C
5270 GGCGTC
Alw44I 980 GTGCAC G^TGCAC
4491 GTGCAC
ApaLI 980 GTGCAC G^TGCAC
4491 GTGCAC
Asp718I 3703 GGTACC G^GTACC
5769 GGTACC
BamHI 1549 GGATCC G^GATCC
4468 GGATCC
BanI 3703 GGTACC G^G[CT][GA]CC
5769 GGTACC
BsaHI 5060 GACGCC G[GA]^CG[CT]C
5270 GGCGTC
Bsp1407I 3694 TGTACA T^GTACA
3709 TGTACA
BsrGI 3694 TGTACA T^GTACA
3709 TGTACA
BstAPI 3479 GCAGTACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3637 GCATGAGCTGC
BstEII 1178 GGTGACC G^GT[ACGT]ACC
2880 GGTGACC
BstPI 1178 GGTGACC G^GT[ACGT]ACC
2880 GGTGACC
DraI 2580 TTTAAA TTT^AAA
5907 TTTAAA
EcoNI 2405 CCTTTTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
5105 CCTGCCTGAGG
EcoO65I 1178 GGTGACC G^GT[ACGT]ACC
2880 GGTGACC
FauI 5380 CCCGC CCCGC(4/6)
4129 GCGGG
Hin1I 5060 GACGCC G[GA]^CG[CT]C
5270 GGCGTC
KpnI 3703 GGTACC GGTAC^C
5769 GGTACC
NcoI 37 CCATGG C^CATGG
2812 CCATGG
PshAI 1573 GACATCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4960 GACAGATGTC
SspBI 3694 TGTACA T^GTACA
3709 TGTACA
#########################################
# 3 OR MORE CUT ENZYMES ( 132 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApoI
Asp700I AspHI AvaI AvaII BalI
BanII BbsI BbvI BciVI BcnI
BfaI BlpI BmyI BpmI Bpu1102I
BpuAI BsaBI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspHI BspMI BsrDI BsrI
BssKI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DraIII
DrdI DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RcaI RsaI SacI Sau3AI
Sau96I ScrFI SexAI SfaNI SfcI
SmaI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI XmnI