##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AccI AccIII AgeI
AscI Asp718I AvrII BamHI BbrPI
BbsI BglII BlnI BpuAI BsaWI
BseAI BsiEI BsiWI BspDI BspEI
BsrBI BsrFI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Cfr10I ClaI
CpoI DraIII DrdI EagI EclXI
Eco52I EcoO65I FseI HpaI KpnI
MluI MroI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PvuI RsrII SacI
SalI SapI SfiI SfuI SgrAI
SmaI SwaI Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AclI 2171 AACGTT AA^CGTT
AcyI 1027 GGCGCC G[GA]^CG[CT]C
Alw44I 3779 GTGCAC G^TGCAC
Aor51HI 509 AGCGCT AGC^GCT
ApaLI 3779 GTGCAC G^TGCAC
AvaI 2042 CCCGAG C^[CT]CG[GA]G
AviII 5437 TGCGCA TGC^GCA
BbeI 1027 GGCGCC GGCGC^C
BcgI 987 CGATTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2074 TGATCA T^GATCA
BglI 6278 GCCAATAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 4945 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1027 GGCGCC G[GA]^CG[CT]C
BsoBI 2042 CCCGAG C^[CT]CG[GA]G
BspLU11I 1397 ACATGT A^CATGT
BssSI 2965 CACGAG CACGAG(-5/-1)
BstAPI 1924 GCAGCATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DsaI 5996 CCGCGG C^C[GA][CT]GG
Eco47III 509 AGCGCT AGC^GCT
EcoRV 1566 GATATC GAT^ATC
FbaI 2074 TGATCA T^GATCA
FspI 5437 TGCGCA TGC^GCA
Hin1I 1027 GGCGCC G[GA]^CG[CT]C
KasI 1027 GGCGCC G^GCGCC
KspI 5996 CCGCGG CCGC^GG
MamI 4945 GATAAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 1027 GGCGCC GG^CGCC
PshAI 1965 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2171 AACGTT AA^CGTT
SacII 5996 CCGCGG CCGC^GG
SbfI 2696 CCTGCAGG CCTGCA^GG
ScaI 498 AGTACT AGT^ACT
SexAI 11 ACCTGGT A^CC[AT]GGT
SfoI 1027 GGCGCC GGC^GCC
SnaBI 4318 TACGTA TAC^GTA
SpeI 4602 ACTAGT A^CTAGT
Sse8387I 2696 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 1190 CGCG CG^CG
5997 CGCG
AflII 2880 CTTAAG C^TTAAG
5077 CTTAAG
AflIII 301 ACACGT A^C[GA][CT]GT
1397 ACATGT
AseI 1893 ATTAAT AT^TAAT
3048 ATTAAT
AsnI 1893 ATTAAT AT^TAAT
3048 ATTAAT
Asp700I 1786 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2114 GAACAAATTC
AspI 1645 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5272 GACCGGGTC
BciVI 5136 GGATAC GTATCC(6/5)
5250 GTATCC
BfrI 2880 CTTAAG C^TTAAG
5077 CTTAAG
BsaAI 302 CACGTA [CT]AC^GT[GA]
4318 TACGTA
BsmI 4751 GAATGC GAATGC(1/-1)
206 GCATTC
Bsp1407I 127 TGTACA T^GTACA
2864 TGTACA
BspHI 2980 TCATGA T^CATGA
6226 TCATGA
BsrDI 3671 CATTGC GCAATG(2/0)
3078 GCAATG
BsrGI 127 TGTACA T^GTACA
2864 TGTACA
BstUI 1190 CGCG CG^CG
5997 CGCG
DraI 1210 TTTAAA TTT^AAA
6537 TTTAAA
EcoNI 5496 CCTCCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
6061 CCTTGGGAAGG
FauI 709 CCCGC CCCGC(4/6)
5998 GCGGG
HaeII 509 AGCGCT [GA]GCGC^[CT]
1027 GGCGCC
HgaI 1645 GACGC GACGC(5/10)
76 GCGTC
HincII 2565 GTCAAC GT[CT]^[GA]AC
5011 GTCAAC
HindII 2565 GTCAAC GT[CT]^[GA]AC
5011 GTCAAC
MvnI 1190 CGCG CG^CG
5997 CGCG
PflFI 1645 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5272 GACCGGGTC
PshBI 1893 ATTAAT AT^TAAT
3048 ATTAAT
RcaI 2980 TCATGA T^CATGA
6226 TCATGA
SspBI 127 TGTACA T^GTACA
2864 TGTACA
StuI 3757 AGGCCT AGG^CCT
5580 AGGCCT
Tth111I 1645 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
5272 GACCGGGTC
XmnI 1786 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2114 GAACAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AhdI AluI
AlwI AlwNI AocI ApaI ApoI
AspEI AspHI AvaII BalI BanI
BanII BbvCI BbvI BcnI BfaI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EaeI Eam1105I EarI Eco81I EcoO109I
EcoRI EcoRII EcoT14I EcoT22I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HindIII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MfeI MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MunI MvaI MwoI NciI NdeII
NlaIII NlaIV NsiI NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII