##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AccIII AclI AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AvrII
BamHI BciVI BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsiEI BsiWI
Bsp1407I BspEI BspHI BsrGI BssHII
BssSI Bst1107I BstBI BstZ17I CelII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV FseI HindIII HpaI
KspI MamI MluI MroI NdeI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI SacII ScaI
SfiI SfuI SgrAI SpeI SspBI
SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2841 GGTACC G^GTACC
AccI 1612 GTCGAC GT^[AC][GT]AC
AflII 407 CTTAAG C^TTAAG
Alw44I 3996 GTGCAC G^TGCAC
ApaLI 3996 GTGCAC G^TGCAC
Asp718I 2841 GGTACC G^GTACC
AviII 2987 TGCGCA TGC^GCA
BbeI 1638 GGCGCC GGCGC^C
BbrPI 2673 CACGTG CAC^GTG
BcgI 345 GCAACTCTATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 657 TGATCA T^GATCA
BfrI 407 CTTAAG C^TTAAG
BsaI 4341 GAGACC GGTCTC(1/5)
BsmBI 1061 CGTCTC CGTCTC(1/5)
BspDI 1200 ATCGAT AT^CGAT
BsrBI 1508 GAGCGG CCGCTC(-3/-3)
ClaI 1200 ATCGAT AT^CGAT
CpoI 1407 CGGTCCG CG^G[AT]CCG
DraI 4052 TTTAAA TTT^AAA
DraIII 1046 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
FbaI 657 TGATCA T^GATCA
FspI 2987 TGCGCA TGC^GCA
KasI 1638 GGCGCC G^GCGCC
KpnI 2841 GGTACC GGTAC^C
MfeI 712 CAATTG C^AATTG
MunI 712 CAATTG C^AATTG
NarI 1638 GGCGCC GG^CGCC
PmaCI 2673 CACGTG CAC^GTG
PmlI 2673 CACGTG CAC^GTG
RsrII 1407 CGGTCCG CG^G[AT]CCG
SalI 1612 GTCGAC G^TCGAC
SbfI 3018 CCTGCAGG CCTGCA^GG
SexAI 2034 ACCAGGT A^CC[AT]GGT
SfoI 1638 GGCGCC GGC^GCC
SnaBI 4310 TACGTA TAC^GTA
Sse8387I 3018 CCTGCAGG CCTGCA^GG
SspI 4504 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AatII 551 GACGTC GACGT^C
2432 GACGTC
AccII 77 CGCG CG^CG
153 CGCG
AocI 2603 CCTGAGG CC^T[ACGT]AGG
2906 CCTCAGG
AspI 1740 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2033 GACCAGGTC
BglII 297 AGATCT A^GATCT
3391 AGATCT
BstAPI 1792 GCAGACCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4421 GCAAGACGTGC
BstEII 560 GGTTACC G^GT[ACGT]ACC
2131 GGTTACC
BstPI 560 GGTTACC G^GT[ACGT]ACC
2131 GGTTACC
BstUI 77 CGCG CG^CG
153 CGCG
BstXI 939 CCACAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1167 CCAGCTTCCTGG
Bsu36I 2603 CCTGAGG CC^T[ACGT]AGG
2906 CCTCAGG
DrdI 1862 GACATGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3754 GACCCCCTTGTC
Eco81I 2603 CCTGAGG CC^T[ACGT]AGG
2906 CCTCAGG
EcoO65I 560 GGTTACC G^GT[ACGT]ACC
2131 GGTTACC
EcoT22I 396 ATGCAT ATGCA^T
1104 ATGCAT
MvnI 77 CGCG CG^CG
153 CGCG
NaeI 14 GCCGGC GCC^GGC
845 GCCGGC
NcoI 2127 CCATGG C^CATGG
2166 CCATGG
NgoMIV 14 GCCGGC G^CCGGC
845 GCCGGC
NsiI 396 ATGCAT ATGCA^T
1104 ATGCAT
PflFI 1740 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2033 GACCAGGTC
PflMI 666 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2311 CCAGTTTGTGG
PpuMI 2159 AGGACCT [GA]G^G[AT]CC[CT]
2402 GGGACCT
SacI 1547 GAGCTC GAGCT^C
2552 GAGCTC
TfiI 245 GAATC G^A[AT]TC
1261 GATTC
Tth111I 1740 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2033 GACCAGGTC
Van91I 666 CCATGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2311 CCAGTTTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 126 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApaI ApoI
Asp700I AspHI AvaI AvaII BalI
BanI BanII BbsI BbvCI BbvI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BspMI BsrDI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
SmlI SphI StuI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII XmaCI XmaI
XmnI